Basic studies for a novel validation system of thyroid hormone-like activities by chemicals

Research Project

Project/Area Number	21590671
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Hygiene
Research Institution	Sagami Women's University
Principal Investigator	OKABE Toshiko 相模女子大学, 栄養科学部, 教授 (20152564)
Project Period (FY)	2009 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords	環境保健 / 甲状腺ホルモン / 甲状腺ホルモン受容体 / 緑色蛍光タンパク質 / GFP / 蛍光強度 / 甲状腺ホルモン応答配列 / バイオアッセイ / 培養細胞 / プロテアソーム阻害剤
Research Abstract	In this study, it was demonstrated that thyroid hormone caused thyroid hormone receptor degradation specifically using the reduction of fluorescent intensities of thyroid hormone receptor-green fluorescent protein(GFP) fusion protein in cultured cells. The reduction of fluorescent intensities was dependent on the concentration of thyroid hormone in the culture medium and this reduction could be detected even in the presence of serum concentration level of thyroid hormone. From these results, it was suggested that the bioassay system using the reduction of fluorescent intensities might be more sensitive than that using transactivation by thyroid hormone receptor and thyroid hormone response element.