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Sensitive detection of hepatitis E virus(HEV) using TaqMan RT-PCR and molecular characterization of HEV in clinical, environmental and putative animal sou rces

Research Project

Project/Area Number 21590725
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Public health/Health science
Research InstitutionHokkaido Institute of Public Health

Principal Investigator

ISHIDA Setsuko  北海道立衛生研究所, 感染症センター・感染症部, 主査(腸管系ウイルス) (70414315)

Co-Investigator(Kenkyū-buntansha) YOSHIZUMI Shima  北海道立衛生研究所, 感染症センター・感染症部, 研究職員 (90414317)
MIYOSHI Masahiro  北海道立衛生研究所, 感染症センター・感染症部, 主査(ウイルス感染症) (50414321)
KUDO Shinichi  北海道立衛生研究所, 感染症センター・微生物部, 部長 (00414318)
Co-Investigator(Renkei-kenkyūsha) MATSUBAYASHI Keiji  北海道赤十字血液センター, 検査部, 検査三課長 (90533323)
GOTO Akiko  北海道立衛生研究所, 感染症センター・感染症部, 研究職員 (60414322)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords疫学 / E型肝炎ウイルス / 診断 / 分子疫学 / ウイルス検出 / 感染症
Research Abstract

A TaqMan RT-PCR was developed to detect and classify genotypes of hepatitis E virus(HEV). Putative animal reservoirs and environmental samples were studied to investigate potential routes of transmission for indigenous HEV infection. HEV RNA was detected using RT-PCR. HEV strains derived from swine liver, environmental samples shared 93-100% sequence similarity with human HEV strains.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (17 results)

All 2012 2011 2010 2009

All Journal Article (8 results) (of which Peer Reviewed: 7 results) Presentation (9 results)

  • [Journal Article] Usefulness of the rapid determination system of viral genome sequences in human stool specimens2012

    • Author(s)
      Miyoshi M, Yoshizumi S, Ishida S, Komagome R, Nagano H, Kudo S, Okano M
    • Journal Title

      J Virol Methods

      Volume: 179 Pages: 256-260

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/22123408

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 二枚貝関連の食中毒疑い事例における各種胃腸炎ウイルスの関与-北海道2011

    • Author(s)
      吉澄志磨、後藤明子、石田勢津子、野田衛
    • Journal Title

      病原微生物得検出情報

      Volume: 32 Pages: 361-363

    • URL

      http://idsc.nih.go.jp/iasr/32/382/dj3827.html

    • Related Report
      2011 Final Research Report
  • [Journal Article] A Case of Paralytic Poliomyelitis Associated with Poliovirus Vaccine Strains in Hokkaido, Japan2010

    • Author(s)
      Miyoshi M, Yoshizumi S, Jinushi M, Ishida S, Okui T, Okano M, Shouji M, Tanaka S, Saigusa J, Mori A, Tanabe H, Yamaguchi R, Nishimura Y, Shimizu H
    • Journal Title

      Jpn J Infect Dis

      Volume: 63 Pages: 216-217

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/20495280

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Different genotypic sapovirusesdetected in two simultaneous outbreaksof gastroenteritis among schoolchildren in the same school district in Hokkaido, Japan2010

    • Author(s)
      Miyoshi M, Yoshizumi S, Kanda N, KarinoT, Nagano H, Kudo S, Okano M, Ishida S
    • Journal Title

      Jpn J Infect Dis

      Volume: 63 Pages: 75-78

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/20093770

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of noroviruses in fecal specimens by direct RT-PCR without RNA purification2010

    • Author(s)
      Nishimura N, Nakayama H, Yoshizumi S, Miyoshi M, Tonoike H, Shirasaki Y, Kojima K, Ishida S
    • Journal Title

      Journal of Virological Methods

      Volume: 163 Pages: 282-286

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/19878699

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Case of Paralytic Poliomyelitis Associated with Poliovirus Vaccine Strains in Hokkaido, Japan2010

    • Author(s)
      Miyoshi M, Yoshizumi S, Jinushi M, Ishida S, Okui T, Okano M, Shouji M, Tanaka S, Saigusa J, Mori A, Tanabe H, Yamaguchi R, Nishimura Y, Shimizu H
    • Journal Title

      Japanese Journal of Infectious Disease

      Volume: 63(3) Pages: 216-217

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Different genotypic sapoviruses detected in two simultaneous outbreaks of gastroenteritis among schoolchildren in the same school district in Hokkaido, Japan2010

    • Author(s)
      Miyoshi M, Yoshizumi S, Kanda N, Karino T, Nagano H, Kudo S, Okano M, Ishida S
    • Journal Title

      Japanese Journal of Infectious Disease 63

      Pages: 75-78

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of noroviruses in fecal specimens by direct RT-PCR without RNA purification2010

    • Author(s)
      Nishimura N, Nakayama H, Yoshizumi S, Miyoshi M, Tonoike H, Shirasaki Y, Kojima K, Ishida S
    • Journal Title

      Journal of Virological Methods 163

      Pages: 282-286

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 北海道におけるHEV感染の概況について2011

    • Author(s)
      石田勢津子
    • Organizer
      第29回ウイルス肝炎談話会
    • Place of Presentation
      自治医大(栃木県下野市)
    • Year and Date
      2011-11-19
    • Related Report
      2011 Final Research Report
  • [Presentation] 北海道におけるHEV感染の概況について2011

    • Author(s)
      石田勢津子
    • Organizer
      第29回ウイルス肝炎談話会
    • Place of Presentation
      自治医大(栃木県下野市)(招待講演)
    • Year and Date
      2011-11-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] 二枚貝の喫食のみられた食中毒疑い事例における各種胃腸炎ウイルスの関与について2011

    • Author(s)
      吉澄志磨,後藤明子,石田勢津子,田中智之,野田衛
    • Organizer
      第32回日本食品微生物学会学術総会
    • Place of Presentation
      タワーホール船堀(東京)
    • Year and Date
      2011-10-07
    • Related Report
      2011 Final Research Report
  • [Presentation] Usefulness of the rapid determination system of viral genome sequences in human stool specimens2011

    • Author(s)
      Miyoshi M, Yoshizumi S, Ishida S, Komagome R, Nagano H, Kudo S, Okano M
    • Organizer
      国際ウイルス学会議(International Union of Microbiological Societies 2011 Congress)
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2011-09-15
    • Related Report
      2011 Final Research Report
  • [Presentation] Detection and molecular characterization of hepatitis E virus in clinical and environmental samples and in putative animal reservoirs2011

    • Author(s)
      Ishida S, Yoshizumi S, Ikeda T, Miyoshi M, GotoA, Matsubayashi K, IkedaH, Kudo S
    • Organizer
      国際ウイルス学会議(International Union of Microbiological Societies 2011 Congress)
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2011-09-13
    • Related Report
      2011 Final Research Report
  • [Presentation] 北海道におけるHEV感染の概況について2011

    • Author(s)
      石田勢津子
    • Organizer
      北海道E型肝炎研究会第5回学術集会
    • Place of Presentation
      札幌医大(札幌市)
    • Year and Date
      2011-09-10
    • Related Report
      2011 Final Research Report
  • [Presentation] 北海道におけるHEV感染の概況について2011

    • Author(s)
      石田勢津子
    • Organizer
      北海道E型肝炎研究会第5回学術集会
    • Place of Presentation
      札幌医大(札幌市)(招待講演)
    • Year and Date
      2011-09-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] Detection and molecular characterization of hepatitis E virus in clinical and environmental samples and in putative animal reservoirs2011

    • Author(s)
      Ishida S, Yoshizumi S, Ikeda T, Miyoshi M, Goto A, Matsubayashi K, Ikeda H, Kudo S
    • Organizer
      国際ウイルス学会議(International Union of Microbiological Societies 2011 Congress)
    • Place of Presentation
      札幌コンベンションセンター(札幌市)(ポスター)(口演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 糞便直接RT-PCR法によるサポウイルスRNA検出法の開発2009

    • Author(s)
      中山博之、吉澄志磨、三好正浩、西村直行、石田勢津子
    • Organizer
      第57回日本ウイルス学会学術集会
    • Place of Presentation
      都市センターホテル(東京)
    • Year and Date
      2009-10-26
    • Related Report
      2011 Final Research Report 2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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