Development of an early differential diagnosis of acute myocardial infarction using the assay of serum Danes llevel
| Project/Area Number |
21590732
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Gunma University |
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Principal Investigator |
NAKAJIMA Tamiko 群馬大学, 大学院・医学系研究科, 助教 (40008561)
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| Co-Investigator(Kenkyū-buntansha) |
KOMINATO Yoshihiko 群馬大学, 大学院・医学系研究科, 教授 (30205512)
SANO Rie 群馬大学, 大学院・医学系研究科, 助教 (70455955)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 心筋梗塞 / DNase I / 診断マーカー / ELISA / 酵素 |
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| Research Abstract |
DNase I activity was reported to increase in the early phase after onset of acute myocardial infarction (AMI). Up to now, DNase I activity has been quantified by the single radial enzyme diffusion (SRED) method, which requires a long incubation time. We have developed a faster and sensitive ELISA capable of measuring DNase I protein concentrations. A significant correlation was observed between DNase I concentration and enzyme activity. The average of serum DNase I in AMI patients within 0.12 h after chest pain was significantly higher than that in healthy individuals. Usefulness of this method in early diagnosis of AMI has been confirmed. To elucidate the mechanism for the elevation of serum DNase I activity induced by ischemia during AMI, we examined regulatory mechanism of hypoxia-induced DNase I gene expression in QGP-1 cells.
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Report
(4 results)
Research Products
(83 results)
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[Journal Article] Nonsynonymous single-nucleotide polymorphisms of the human apoptosis-related endonuclease-DNA fragmentation factor beta polypeptide, endonuclease G, and Flap endonuclease-1-genes show a lowdegree of genetic heterogeneity2012
Author(s)
Takeshita H, Fujihara J, Ueki, M, Iida R, Koda Y, Soejima M, Yuasa I, Kato H, Nakajima T, Kominato Y and Yasuda T
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Journal Title
DNA Cell Biol
Volume: 31
Pages: 36-42
Related Report
Peer Reviewed
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[Journal Article] Identification of the Brackish Water Clam Corbicula Japonica (Japanese Name, Yamato-Shijimi) and Specification of the Growing District by Polymerase Chain Reaction (PCR)-Based Analysis of Mitochondrial DNA2011
Author(s)
Fujihara J, Yasuda T, Ueki M, Fujita Y, Nakamura M, Oshiumi C, Hosozawa T, Nabika T, Harada Y, Kobayashi T, Nakajima T and Takeshita H
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Journal Title
Environmental Forensics
Volume: 12
Pages: 156-161
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Peer Reviewed
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[Journal Article] A case of fatal drug intoxication showing a high-density duodenal content by postmortem computed tomography.2011
Author(s)
Sano R, Takahashi K, Kominato Y, Araki T, Yamamoto K, Takei H, Otake H, Awata S, Akuzawa H, Tago Y, Aoki H
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Journal Title
Leg Med (Tokyo).
Volume: 13
Pages: 39-40
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Peer Reviewed
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[Journal Article] 低酸素による核酸分解酵素DNASEI遺伝子の転写調節2010
Author(s)
佐野利恵, 中島たみ子, 小湊慶彦, 加藤恵理子, 高士祐一, 新田みなみ, 山根庸弘, 藤原純子, 竹下治男, 植木美鈴, 安田年博
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Journal Title
DNA多型
Volume: 18
Pages: 278-282
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Peer Reviewed
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[Journal Article] Development of a sensitive enzyme-linked immunosorbent assay for measurement of DNase I in human serum2009
Author(s)
Nakajima T, Takagi R, Tajima Y, Makita C, Kominato Y, Kuribara J, Ohshima S, Tada H, Tsurugaya H, Kobayashi Y, Takeshita H, Kawai Y and Yasuda T.
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Journal Title
Clinica Chimica Acta
Volume: 403
Pages: 219-222
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Peer Reviewed
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[Journal Article] Caucasian-specific allele in non-synonymous single nucleotide polymorphisms of the gene encoding deoxyribonuclease I-like 3, potentially relevant to autoimmunity, produces an inactive enzyme2009
Author(s)
Ueki M, Takeshita H, Fujihara J, Iida R, Yuasa I, Kato H, Panduro A, Nakajima T, Kominato Y and Yasuda T
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Journal Title
Clinica Chimica Acta
Volume: 407
Pages: 20-24
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Peer Reviewed
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[Journal Article] Levels of serum deoxyribonuclease I activity on admission in patients with acute myocardial infarction can be useful in predicting left ventricular enlargement due to remodeling2009
Author(s)
Kuribara J, Tada H, Kawai Y, Kawaguchi R, Hoshizaki H, Arakawa K, Kitayama M, Kajinami K, Kurabayashi M, Oshima S, Taniguchi K, Kominato Y and Yasuda T
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Journal Title
Journal of Cardiology
Volume: 53
Pages: 196-203
NAID
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Peer Reviewed
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[Journal Article] 核酸分解酵素DNaseI遺伝子の転写調節機構の解明2009
Author(s)
高木利恵, 中島たみ子, 小湊慶彦, 加藤恵理子, 高士祐一, 新田みなみ, 山根庸弘, 藤原純子, 竹下治男, 植木美鈴, 安田年博
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Journal Title
DNA多型
Volume: 17
Pages: 177-179
NAID
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Peer Reviewed
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[Journal Article] Expression of ABO blood-group genes is dependent upon an erythroid cell-specific regulatory element, which is deleted in individuals with the B_m phenotype
Author(s)
Sano R, Nakajima T, Takahashi K, Kubo R, Kominato Y, Tsukada J, Takeshita H, Yasuda T, Ito K, Maruhashi T, Yokohama A, Isa K, Ogasawara K, Uchikawa M
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Peer Reviewed
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[Journal Article] Genetic variation of FUT2z in a Vietnamese population : identification of two novel Se enzyme.inactivating mutations
Author(s)
Soejima M, Fujimoto R, Agusa T, Iwata H, Fujihara J, Takeshita H, Minh TB, Trang PT, Viet PH, Nakajima T, Yoshimoto J, Tanabe S, Koda Y
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Journal Title
Transfusion
Volume: (in press)
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Peer Reviewed
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[Presentation] 低酸素による核酸分解酵素DNASEI遺伝子の転写調節2009
Author(s)
佐野利恵, 中島たみ, 小湊慶彦, 加藤恵理子, 高士祐一, 山根庸弘, 新田みなみ, 藤原純子, 竹下治男, 植木美鈴, 安田年博
Organizer
日本DNA型学会第18回学術集会
Place of Presentation
久留米
Year and Date
2009-11-20
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