| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
DNase I activity was reported to increase in the early phase after onset of acute myocardial infarction (AMI). Up to now, DNase I activity has been quantified by the single radial enzyme diffusion (SRED) method, which requires a long incubation time. We have developed a faster and sensitive ELISA capable of measuring DNase I protein concentrations. A significant correlation was observed between DNase I concentration and enzyme activity. The average of serum DNase I in AMI patients within 0.12 h after chest pain was significantly higher than that in healthy individuals. Usefulness of this method in early diagnosis of AMI has been confirmed. To elucidate the mechanism for the elevation of serum DNase I activity induced by ischemia during AMI, we examined regulatory mechanism of hypoxia-induced DNase I gene expression in QGP-1 cells.
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