| Project/Area Number |
21590745
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Tokai University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | パラコート / グリホサート / 有機リン / 固相抽出 / 一斉分析 / カーバメイト / モノリス / LC-MS/MS / ジクワット / グルホシネート / モノリススピンカラム / GC-MS / フェニトロチオン |
|---|
| Research Abstract |
We have developed a method based on monolitic spin column extraction and gas chromatography-mass spectrometry(GC-MS) as an analytical method for screening diquat, paraquat, and fenitrothion(MEP) in serum and urine. Then, a rapid, specific, and sensitive method for simultaneous quantitation of organophosphates [MEP, malathion, and phenthoate], glufosinate, and glyphosate in human serum and urine by GC-MS has been developed. Finally, a liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous quantification of sixteen organophosphate and carbamate insecticides in serum. Non-polar and polar pesticides were simultaneously extracted from serum samples by a simple and fast monolithic spin column procedure using mix-mode C-C18 cartridges. The validated all method was successfully applied to a clinical poisoning case.
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