Simultaneous analysis of paraquat, glufosinate and organophaophate in biological fluid using solid-phase extraction

• Project/Area Number: 21590745
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Tokai University
• Principal Investigator: SAITO Takeshi 東海大学, 医学部, 准教授 (30266465)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: パラコート / グリホサート / 有機リン / 固相抽出 / 一斉分析 / カーバメイト / モノリス / LC-MS/MS / ジクワット / グルホシネート / モノリススピンカラム / GC-MS / フェニトロチオン

Research Abstract

We have developed a method based on monolitic spin column extraction and gas chromatography-mass spectrometry(GC-MS) as an analytical method for screening diquat, paraquat, and fenitrothion(MEP) in serum and urine. Then, a rapid, specific, and sensitive method for simultaneous quantitation of organophosphates [MEP, malathion, and phenthoate], glufosinate, and glyphosate in human serum and urine by GC-MS has been developed. Finally, a liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous quantification of sixteen organophosphate and carbamate insecticides in serum. Non-polar and polar pesticides were simultaneously extracted from serum samples by a simple and fast monolithic spin column procedure using mix-mode C-C18 cartridges. The validated all method was successfully applied to a clinical poisoning case.

Research Products

• [Journal Article] Mix-mode C-C18 monolithic spin column extraction and GC-MS for simultaneous assay of organophosphorus compounds, glyphsate, and glufosinate in human serum and urine (2012)
  • Author(s): Saito T, Miura N, Namera A, Oikawa H, Miyazaki S, Nakamoto A and Inokuchi S
  • Journal Title: Forensic Toxicol Volume: 30 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Mix-mode C-C_<18> monolithic spin-column extraction and GC-MS for simultaneous assay of organophosphorus compounds, glufosinate, and glyphosate in human serum (2012)
  • Author(s): Saito, T., Miura, N., 他5名
  • Journal Title: Forensic Toxicology Volume: 30 Issue: 1 Pages: 1-10
  • DOI: 10.1007/s11419-011-0120-7
  • Peer Reviewed
• [Journal Article] Mix-mode TiO-C18 monolith spin column extraction and GC-MS for the simultaneous assay of organophosphorus compounds and glufosinate, and glyphosate in human serum and urine (2011)
  • Author(s): Saito T, Aoki H, Namera A, Oikawa H, Miyazaki S, Nakamoto A and Inokuchi S
  • Journal Title: Anal Sci Volume: 27 Pages: 999-1005
  • NAID: 130004827065
  • Peer Reviewed
• [Journal Article] Monolithic spin column extraction and GC-MS for the simultaneous assay of diquat, paraquat, and fenitrothion in human serum and urine (2011)
  • Author(s): Saito T, Fukushima T, Yui Y, Miyazaki S, Nakamoto A, Namera A and Inokuchi S
  • Journal Title: Anal Bioanal Chem Volume: 400(1) Pages: 25-31
  • Peer Reviewed
• [Journal Article] Mix-mode TiO-C18 Monolith Spin Column Extraction and GC-MS for the Simultaneous Assay of Organophosphorus Compounds and Glufosinate, and Glyphosate in Human Serum and Urine (2011)
  • Author(s): Saito, T., Aoki, H., 他5名
  • Journal Title: Analytical Sciences Volume: 27 Issue: 10 Pages: 999
  • DOI: 10.2116/analsci.27.999
  • NAID: 130004827065
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed
• [Journal Article] Monolithic Spin column extraction and GC-MS for the simultaneous assay of diquat, paraquat, and fenitrothion in human serum and urine (2011)
  • Author(s): T.Saito, T.Fukushima, Y.Yui, S.Miyazaki, A.Nakamoto, A.Namera, S.Inokuchi
  • Journal Title: Anal Bioanal Chem Volume: 400 Pages: 25-31
  • Peer Reviewed
• [Presentation] Mix-mode TiO-C18 monolith spin column extraction and GC-MS analysis for the simultaneous assay of organophosphorus compounds, glufosinate and glyphosate in human serum and urine (2011)
  • Author(s): Saito T, Namera A, Oikawa H, Miyazaki S, Nakamoto A and Inokuchi S
  • Organizer: Joint meeting of the society of forensic toxicologists & the international association of forensic toxicologists
  • Place of Presentation: Marriot Marquis Hotel(San Francisco)
  • Year and Date: 2011-09-28
• [Presentation] Mix-mode TiO-C18 monolith spin column extraction and GC-MS analysis for the simultaneous assay of organophosphorus compounds, glufosinate and glyphosate in human serum and urine (2011)
  • Author(s): Saito, T., Namera, A., 他4名
  • Organizer: Joint Meeting of the Society of Forensic Toxicologists & the International Association of Forensic Toxicologists
  • Place of Presentation: サンフランシスコ(アメリカ)
  • Year and Date: 2011-09-28
• [Presentation] ミックスモードモノスピン抽出とGC-MSによる血清、尿中有機リン、グリホサート、グルホシネートの一斉分析 (2011)
  • Author(s): 斉藤剛, 奈女良昭, 宮崎将太, 及川博史, 中本晃弘, 猪口貞樹
  • Organizer: 第33回日本中毒学会総会・学術総会
  • Place of Presentation: ソフトピアジャパン(大垣市)
  • Year and Date: 2011-07-31
• [Presentation] ミックスモードモノスピン抽出とGC-MSによる血清、尿中有機リン、グリホサート、グルホシネートの一斉分析 (2011)
  • Author(s): 斉藤剛, 奈女良昭, 他4名
  • Organizer: 第33回日本中毒学会総会・学術集会
  • Place of Presentation: ソフトピアジャパン(岐阜県)
  • Year and Date: 2011-07-31
• [Presentation] モノリススピンカラムとGC-MSによる体液中有機リン、グリホサート、グルホシネートの同時分析 (2011)
  • Author(s): 斉藤剛, 奈女良昭
  • Organizer: 第95次日本法医学会学術全国集会
  • Place of Presentation: コラッセ福島(福島市)
  • Year and Date: 2011-06-16
• [Presentation] モノリススピンカラムとGC-MSによる体液中有機リン、グリホサート、グルホシネートの同時分析 (2011)
  • Author(s): 斉藤剛, 奈女良昭
  • Organizer: 第95次日本法医学会学術全国集会
  • Place of Presentation: コラッセふくしま(福島都)
  • Year and Date: 2011-06-16
• [Presentation] ミックスモードC-C18モノリススピンカラムとGC-MSによる血清、尿中有機リン、グルホシネート、グリホサートの一斉分析 (2011)
  • Author(s): 斉藤剛, 奈女良昭, 及川博史, 宮崎将太, 中本晃弘, 猪口貞樹
  • Organizer: 日本法中毒学会第30年会
  • Place of Presentation: 長崎大学(長崎市)
  • Year and Date: 2011-06-10
• [Presentation] Monolithic spin-column extraction and gas chromatography-mass spectrometry method for the simultaneous assay of diquat, paraquat, and fenitrothion in human serum and urine (2010)
  • Author(s): Saito, T., Namera, A., Yui, Y., Miyazaki, S., Nakamoto, A. and Inokuchi, S
  • Organizer: The International Association of Forensic Toxcologists 48th International Meeting
  • Place of Presentation: Bonn Castle(Bonn)
  • Year and Date: 2010-09-30
• [Presentation] モノリススピンカラムによる体液中ジクワット、パラコート、フェニトロチオンの抽出とGC-MSの同時分析 (2010)
  • Author(s): 斉藤剛, 奈女良昭, 由井夕湖, 宮崎将太, 中本晃弘, 猪口貞樹
  • Organizer: 第32回日本中毒学会総会・学術集会
  • Place of Presentation: 川崎学園(倉敷市)
  • Year and Date: 2010-07-23
• [Presentation] モノリススピンカラムによる体液中ジクワット、パラコート、フェニトロチオンの抽出とGC-MSの同時分析 (2010)
  • Author(s): 斉藤剛、奈女良昭、由井夕湖、宮崎将太、中本晃弘、猪口貞樹
  • Organizer: 第32回日本中毒学会総会・学術集会
  • Place of Presentation: 川崎学園川崎祐宣記念講堂(岡山県)
  • Year and Date: 2010-07-23
• [Presentation] モノリススピンカラムとGC-MSによる体液中ジクワット,パラコート,フェニトロチオンの同時分析 (2010)
  • Author(s): 斉藤剛, 奈女良昭
  • Organizer: 第94次日本法医学会学術全国集会
  • Place of Presentation: タワーホール船堀(江戸川区)
  • Year and Date: 2010-06-24
• [Presentation] モノリススピンカラムとGC-MSによる体液中ジクワット、パラコート、フェニトロチオンの同時分析 (2010)
  • Author(s): 斉藤剛、奈女良昭
  • Organizer: 第94次日本法医学会学術全国集会
  • Place of Presentation: タワーホール船堀(東京都)
  • Year and Date: 2010-06-24
• [Presentation] Monolithic spin column extraction and gas chromatography mass spectrometry method for the simultaneous assay of diquat, paraquat, and feintrothion in human serum and urine (2010)
  • Author(s): T.Saito, A.Namera, Y.Yui, S.Miyazaki, A.Nakamoto, S.Inokuchi
  • Organizer: 48th Annual Meeting of the International Association of Forensic Toxicologists(TIAFT)
  • Place of Presentation: ベートーベンホール(ボン)