Kinship analysis based on massive SNP genotype data

• Project/Area Number: 21590746
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Tokai University
• Principal Investigator: OSAWA Motoki 東海大学, 医学部, 教授 (90213686)
• Co-Investigator(Kenkyū-buntansha): KIMURA Ryousuke 琉球大学, 亜熱帯島嶼科学超域研究推進機構, 特命准教授 (00453712)
• Co-Investigator(Renkei-kenkyūsha): INAOKA Yoshihiko 東海大学, 医学部, 助教 (50564689)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 親子鑑定 / DNA多型 / SNP / マイクロアレイ / 遺伝子

Research Abstract

The multiplex system of 15 STR loci has been employed in kinship analysis, but its power is insufficient even for sibship. The microarray technique allows us to obtain massive SNP genotype data. As indices for the evaluation based on SNP, the proportion of identical-by-decent(IBD) numbers was preferable, in which linkage disequilibrium and minor allele frequency were conditioned. The minimum number to separate kinship from unrelated pairs was estimated to be approximately 500 and several thousands of SNPs for siblings and the second degree, respectively.

Research Products

• [Journal Article] IBS値に基づく血縁関係解析法の実用性 (2011)
  • Author(s): 田村友紀, 木村亮介, 稲岡斉彦, 田中佐知, 佐藤文子, 佐藤至, 大澤資樹
  • Journal Title: DNA多型 Volume: 19 Pages: 179-184
  • NAID: 10029668842
• [Journal Article] DNAチップを用いたSNP解析による二者間の血縁関係判定 (2011)
  • Author(s): 稲岡斉彦, 田嶋敦, 田村友紀, 田中佐知, 佐藤文子, 筒井健太, 佐藤至, 大澤資樹
  • Journal Title: DNA多型 Volume: 19 Pages: 164-168
  • NAID: 10029668815
• [Journal Article] Kinship analysis based on SNP data from microarray assay (2011)
  • Author(s): Inaoka Y, Tajima A, Tamura T, Satoh F, Osawa M
  • Journal Title: Forensic Sci Int. Genetics Volume: 3(1)
• [Journal Article] Re-evaluation of the identical-by-state method in pairwise kinship inference (2011)
  • Author(s): Tamura T, Osawa M, Inaoka Y, Tanaka S, Satoh F, Nakamura T
  • Journal Title: Forensic Sci Int. Genetics Volume: 3(1)
• [Journal Article] Postmortem molecular analysis to SIDS victims (2011)
  • Author(s): Osawa M, Inaoka Y, Hasegawa I, Satoh F
  • Journal Title: Forensic Sci Int. Genetics Volume: 3(1)
• [Journal Article] Postmortem molecular analysis to SIDS victims (2011)
  • Author(s): Osawa M, Inaoka Y, Hasegawa I, Satoh F
  • Journal Title: Forensic Science International : Genetics Volume: 3(1) Issue: 1 Pages: e263-e264
  • DOI: 10.1016/j.fsigss.2011.08.128
  • Peer Reviewed
• [Journal Article] Kinship analysis based on SNP data from microarray assay (2011)
  • Author(s): Inaoka Y, Tajima A, Tamura T, Satoh F, Osawa M
  • Journal Title: Forensic Science International : Genetics Volume: 3(1) Issue: 1 Pages: e275-e276
  • DOI: 10.1016/j.fsigss.2011.08.134
  • Peer Reviewed
• [Journal Article] Re-evaluation of the identical-by-state method in pairwise kinship inference (2011)
  • Author(s): Tamura T, Osawa M, Inaoka Y, Tanaka S, Satoh F, Nakamura T
  • Journal Title: Forensic Science International : Genetics Volume: 3(1) Issue: 1 Pages: e244-e245
  • DOI: 10.1016/j.fsigss.2011.08.121
  • Peer Reviewed
• [Journal Article] IdentifilerシステムでのIBS法による血縁関係解析 (2010)
  • Author(s): 大澤資樹, 田村友紀, 木村亮介, 稲岡斉彦, 早田慈, 長谷川巖, 佐藤文子, 佐藤至
  • Journal Title: DNA多型 Volume: 18 Pages: 152-156
  • NAID: 10029668214
• [Journal Article] A common variation in EDAR is a genetic determinant of shovel-shaped incisors (2009)
  • Author(s): Kimura R, Yamaguchi T, Takeda M, Kondo O, Toma T, Haneji K, Hanihara T, Matsukusa H, Kawamura S, Maki K, Osawa M, Ishida H, Oota H
  • Journal Title: Am J Hum Genet Volume: 85(4) Pages: 528-535
  • Peer Reviewed
• [Journal Article] 集団頻度情報を用いないゲノムワイドなSNPタイピングによる親縁係数の推定 (2009)
  • Author(s): 木村亮介, 大澤資樹
  • Journal Title: DNA多型 Volume: 17 Pages: 14-17
  • NAID: 10025754017
• [Journal Article] Evaluation of the allele-sharing approach, known as the IBS method, in kinship analysis the allele-sharing approach, known as the IBS method
  • Author(s): Tamura T, Osawa M, Kimura R, Inaoka Y, Tanaka S, Satoh F, Sato I
  • Journal Title: Forensic Leg Med Volume: (in press)
  • Peer Reviewed
• [Journal Article] Evaluation of the allele-sharing approach, known as the IBS method, in kinship analysis
  • Author(s): Tamura T., Osawa M., Inaoka S, Kimura R., et al.
  • Journal Title: Journal of Forensic and Legal Medicine Volume: (in press)
  • Peer Reviewed
• [Presentation] 同胞関係推定におけるIBS値の応用 (2011)
  • Author(s): 田村友紀、大澤資樹、稲岡斉彦、田中佐知、佐藤文子
  • Organizer: 日本DNA多型学会第20回学術集会
  • Place of Presentation: 横浜、はまぎんホール
  • Year and Date: 2011-12-02
• [Presentation] ABO式血液型におけるDNA検査による遺伝子型判定法の比較 (2011)
  • Author(s): 稲岡斉彦, 筒井健太, 中西亜由美, 坪井秋男, 瀬戸良久, 長谷川巖, 佐藤文子, 大澤資樹
  • Organizer: 日本DNA多型学会第20回学術集会
  • Place of Presentation: はまぎんホール(横浜市)
  • Year and Date: 2011-12-01
• [Presentation] Kinship analysis based on SNP data from microarray assay (2011)
  • Author(s): Inaoka Y, Tajima A, Tamura T, Satoh F, Osawa M
  • Organizer: 24^<th> World Congress of the International Society for Forensic Genetics
  • Place of Presentation: University of Vienna, Vienna, Austria(招待講演)
  • Year and Date: 2011-09-01
• [Presentation] Postmortem molecular analysis to SIDS victims (2011)
  • Author(s): Osawa M, Inaoka Y, Hasegawa I, Satoh F
  • Organizer: 24^<th> World Congress of the International Society for Forensic Genetics
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2011-08-31
• [Presentation] Postmortem molecular analysis to SIDS victims (2011)
  • Author(s): Osawa M, Inaoka Y, Hasegawa I, Satoh F
  • Organizer: 24^<th> World Congress of the International Society for Forensic Genetics
  • Place of Presentation: University of Vienna, Vienna, Austria
  • Year and Date: 2011-08-31
• [Presentation] Kinship analysis based on SNP data from microarray assay (2011)
  • Author(s): Inaoka Y, Tajima A, Tamura T, Satoh F, Osawa M
  • Organizer: 24^<th> World Congress of the International Society for Forensic Genetics
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2011-08-30
• [Presentation] Re-evaluation of the identical-by-state method in pairwise kinship inference (2011)
  • Author(s): Tamura T, Osawa M, Inaoka Y, Tanaka S, Satoh F, Nakamura T
  • Organizer: 24^<th> World Congress of the International Society for Forensic Genetics
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2011-08-30
• [Presentation] 原因不明の突然死に対する死後遺伝子解析 (2010)
  • Author(s): 大澤資樹
  • Organizer: 日本DNA多型学会第19回学術集会・シンポジウム
  • Place of Presentation: 三島市民文化会館(静岡県)
  • Year and Date: 2010-11-19
• [Presentation] IBS値に基づく血縁関係解析法の実用性 (2010)
  • Author(s): 田村友紀, 木村亮介, 稲岡斉彦, 田中佐知, 佐藤文子, 佐藤至, 大澤資樹
  • Organizer: 日本DNA多型学会第19回学術集会
  • Place of Presentation: 三島市民文化会館(三島市)
  • Year and Date: 2010-11-18
• [Presentation] STR多型解析からの二者間血縁関係判定におけるIBS評価法 (2010)
  • Author(s): 大澤資樹、田村友紀、木村亮介、稲岡斉彦、田中佐知、早田慈、長谷川巌、佐藤文子、佐藤至
  • Organizer: 日本人類遺伝学会第55回大会日本人類遺伝学会第55回大会
  • Place of Presentation: 大宮ソニックシティ(さいたま市)
  • Year and Date: 2010-10-28
• [Presentation] STR多型解析からの二者間血縁関係判定におけるIBS評価法 (2010)
  • Author(s): 大澤資樹
  • Organizer: 日本人類遺伝学会第55回大会
  • Place of Presentation: 大宮ソニックシティ(埼玉県)
  • Year and Date: 2010-10-28
• [Presentation] Postmortem molecular analysis of sudden deaths from unknown causes (2010)
  • Author(s): Osawa M
  • Organizer: BIT's 1st Annual World Congress of Forensics
  • Place of Presentation: Dalian Bayshore Hotel, Dalian, China
  • Year and Date: 2010-10-23
• [Presentation] IdentifilerシステムでのIBS法による血縁関係解析 (2009)
  • Author(s): 大澤資樹
  • Organizer: 日本DNA多型学会
  • Place of Presentation: 久留米大学医学部(福岡県)
  • Year and Date: 2009-11-20
• [Presentation] 個人識別におけるAmpFlSTR Identifiler Kitを用いたSTR解析の有用性 (2009)
  • Author(s): 田村友紀, 大澤資樹, 田村佐和, 木村亮介, 稲岡斉彦, 早田慈, 山本佳世, 石割敦也, 鈴木隆徳, 佐藤至
  • Organizer: 日本法科学技術学会第15回学術集会
  • Place of Presentation: ホテルフロラシオン青山(東京都)
  • Year and Date: 2009-11-12
• [Presentation] IdentifilerシステムでのIBS法による血縁関係解析 (2009)
  • Author(s): 大澤資樹, 田村友紀, 木村亮介, 稲岡斉彦, 早田慈, 長谷川巖, 佐藤文子, 佐藤至
  • Organizer: 日本DNA多型学会第18回学術集会
  • Place of Presentation: 久留米大学医学部筑水会館(久留米市)
  • Year and Date: 2009-10-28
• [Presentation] Evaluation of the IBS method in relationship analysis from short tandem repeat data (2009)
  • Author(s): Osawa M, Kimura R, Tamura T, Soda M, Satoh F, Hasegawa I, Sato I
  • Organizer: The American Society of Human Genetics 59th annual meeting
  • Place of Presentation: Hawaii Convention Center, Honolulu, USA
  • Year and Date: 2009-10-22
• [Presentation] A nonsynonymous SNP in EDAR is associated with tooth shoveling (2009)
  • Author(s): Kimura R, Yamaguchi Y, Maki K, Takeda M, Kondo O, Hanihara T, Osawa M, Ihida H, Kawamura S, Oota H
  • Organizer: The American Society of Human Genetics 59th Annual Meeting
  • Place of Presentation: Hawaii Convention Center, Honolulu, USA
  • Year and Date: 2009-10-22