Analysis of polymorphisms and association study of FUT2 and FUT3, regulating synthesis of Lewis blood group antigens.

• Project/Area Number: 21590750
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Kurume University
• Principal Investigator: SOEJIMA Mikiko 久留米大学, 医学部, 講師 (80279140)
• Co-Investigator(Kenkyū-buntansha): KODA Yoshiro 久留米大学, 医学部, 教授 (90231307)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 糖転移酵素 / 糖鎖 / 血液型 / 遺伝子多型 / ルイス式血液型 / 分泌型 / DNA多型 / 遺伝子組換え / DNAメチル化

Research Abstract

The FUT2 and FUT3 regulate the synthesis of the ABO and Lewis blood group antigens. Recent studies have shown these genes were rich in polymorphisms with population-specific manner. We developed new methods to determine not only SNPs but copy number variations of the FUT2 to promote such studies. In addition, we performed analysis of the transcriptional region of the FUT2 and association study of polymorphisms and lifestyle-related diseases.

Research Products

• [Journal Article] Genetic variation of FUT2 in a Vietnamese population: Identification of two novel Se enzyme - Inactivating mutations (2012)
  • Author(s): Soejima, M.
  • Journal Title: Transfusion Volume: 52(6) Issue: 6 Pages: 1268-1275
  • DOI: 10.1111/j.1537-2995.2011.03485.x
  • Peer Reviewed
• [Journal Article] Selective quantification of human DNA by real-time PCR of FOXP2 (2012)
  • Author(s): Soejima M, Hiroshige K, Yoshimoto J, Koda Y
  • Journal Title: Forensic Sci Int Genet Volume: (In press)
  • Peer Reviewed
• [Journal Article] Nonsynonymous Single-Nucleotide Polymorphisms of the Human Apoptosis-Related Endonuclease-DNA Fragmentation Factor Beta Polypeptide, Endonuclease G, and Flap Endonuclease-1-Genes Show a Low Degree of Genetic Heteroseneity (2012)
  • Author(s): Takeshita H, Fujihara J, Ueki M, Iida R, Koda Y, Soejima M, Yuasa I, Kato H, Nakajima T, Kominato Y, Yasuda T
  • Journal Title: DNA Cell Biol Volume: 1 Pages: 36-42
  • Peer Reviewed
• [Journal Article] TaqMan-based real-time polymerase chain reaction for detection of FUT2 copy number variations : identification of novel Alu-mediated deletion (2011)
  • Author(s): Soejima M, Koda Y
  • Journal Title: Transfusion Volume: 51(4) Pages: 762-769
  • Peer Reviewed
• [Journal Article] Polymorphic trial in oxidative damage of arsenic exposed Vietnamese (2011)
  • Author(s): Fujihara J, Soejima M, Yasuda T, Koda Y, Kunito T, Iwata H, Tanabe S, Takeshita H
  • Journal Title: Toxicol Appl Pharmacol Volume: 256 Pages: 174-178
  • Peer Reviewed
• [Journal Article] Confirmation that SNPs in the high mobility group-A2 gene (HMGA2) are associated with adult height in the Japanese population ; wide-ranging population survey of height-related SNPs in HMGA2 (2011)
  • Author(s): Takeshita H, Fujihara J, Soejima M, Koda Y, Kimura-Kataoka K, Ono R, Yuasa I, Iida R, Ueki M, Nagao M, Yasuda T
  • Journal Title: Electrophoresis Volume: 32 Issue: 14 Pages: 1844-1851
  • DOI: 10.1002/elps.201100128
  • Peer Reviewed
• [Journal Article] Rapid detection of haptoglobin gene deletion in alkaline-denatured blood by loop-mediated isothermal amplification reaction (2011)
  • Author(s): Soejima M, Egashira K, Kawano H, Kawaguchi A, Sagawa K, Koda Y
  • Journal Title: J Mol Diagn Volume: 13 Pages: 334-339
  • Peer Reviewed
• [Journal Article] First survey of the three gene polymorphisms (PON1 Q192R, eNOS E298D and eNOS C-786T) potentially associated with coronary artery spasm in African populations and comparison with worldwide data (2011)
  • Author(s): Fujihara J, Yasuda T, Kawai Y, Morikawa N, Arakawa K, Koda Y, Soejima M, Kimura-Kataoka K, Takeshita H
  • Journal Title: Cell Biochem Funct Volume: 29 Issue: 2 Pages: 156-163
  • DOI: 10.1002/cbf.1721
  • Peer Reviewed
• [Journal Article] Functional and gcnctic surycy of all known single-nucleotide polymuphisms within the human deoxyribonuclease I gene in wide-ranging ethnic groups (2011)
  • Author(s): Fujihara J, Ueki M, Yasuda T, Iida R, Soejima M, Koda Y, Kimura-Kataoka K, Kato H, Panduro A, Tongu M, Takeshita H
  • Journal Title: DNA Cell Biol Volume: 30 Pages: 205-217
  • Peer Reviewed
• [Journal Article] Distribution of 42-bp variable tandem repeal polymorphism of the cold-induced autoinflammatory syndrome 1 (CIAS1) gene in eight human populations (2011)
  • Author(s): Omi T, Koda Y, Soejima M, Munkhtulga L, Iwamoto S
  • Journal Title: Leg Med (Tokyo) Volume: 13 Pages: 44-46
  • Peer Reviewed
• [Journal Article] Rapid Detection of Haptoglobin Gene Deletion in Alkaline-Denatured Blood by Loop-Mediated Isothermal Amplification Reaction. (2011)
  • Author(s): Soejima M, Egashira K, Kawano H, Kawaguchi A, Sagawa K, Koda Y.
  • Journal Title: J Mol Diagnos. Volume: In press
  • Peer Reviewed
• [Journal Article] TaqMan-based real-time polymerase chain reaction for detection of FUT2 copy number variations : identification of novel Alu-mediated deletion. (2011)
  • Author(s): Soejima M, Koda Y
  • Journal Title: Transfusion Volume: In press
  • Peer Reviewed
• [Journal Article] Functional and genetic survey of all known single-nucleotide polymorphisms within the human deoxyribonuclease I gene in wide-ranging ethnic groups. (2011)
  • Author(s): Fujihara J, Ueki M, Yasuda T, Iida R, Soejima M, Koda Y, Kimura-Kataoka K, Kato H, Panduro A, Tongu M, Takeshita H.
  • Journal Title: DNA Cell Biol. Volume: 30(In press)
  • Peer Reviewed
• [Journal Article] First survey of the three gene polymorphisms (PONI Q192R, eNOS E298D and eNOS C-786T) potentially associated with coronary artery spasm in African populations and comparison with worldwide data. (2011)
  • Author(s): Fujihara J, Yasuda T, Kawai Y, Morikawa N, Arakawa K, Koda Y, Soejima M, Kimura-Kataoka K, Takeshita H
  • Journal Title: Cell Biochem Funct Volume: 29 Pages: 56-63
  • Peer Reviewed
• [Journal Article] 輸血副作用原因遺伝子ハプトグロビン欠失アリルの輸血前診断法の検討 (2011)
  • Author(s): 神田芳郎、副島美貴子、川野洋之、江頭弘一、佐川公矯
  • Journal Title: 日本輸血細胞治療学会誌 Volume: 57 Pages: 34-38
  • NAID: 10029418194
  • Peer Reviewed
• [Journal Article] FDistribution of 42-bp variable tandem repeat polymorphism of the cold-induced autoinflammatory syndrome l (CIASI) gene in eight human populations. (2011)
  • Author(s): Omi T, Koda Y, Soejima M, Munkhtulga L, Iwamoto S.
  • Journal Title: Leg Med (Tokyo) Volume: 13 Pages: 44-46
  • Peer Reviewed
• [Journal Article] FGlobal analysis of single nucleotide polymorphisms in the exons of human deoxyribonuclease I-like I and 2 genes. (2011)
  • Author(s): Fujihara J, Yasuda T, Iida R, Kimura-Kataoka K, Soejima M, Koda Y, Kato H, Panduro A, Yuasa I, Takeshita H.
  • Journal Title: Electrophoresis Volume: 31 Pages: 3552-3557
  • Peer Reviewed
• [Journal Article] A biochemical and genetic study on all non-synonymous single nucleotide polymorphisms of the gene encoding human deoxyribonuclease I potentially relevant to autoimmunity. (2010)
  • Author(s): Yasuda T, Ueki M, Takeshita H, Fujihara J, Kimura-Kataoka K, Iida R, Tsubota E, Soejima M, Koda Y, Kato H, Panduro A.
  • Journal Title: Int J Biochem CellBiol. Volume: 42 Pages: 1216-1225
  • Peer Reviewed
• [Journal Article] Development and validation of a SYBR Green I-based real-time polymerase chain reaction method for detection of haptoglobin gene deletion in clinical materials. (2010)
  • Author(s): Soejima M, Tsuchiya Y, Egashira K, Kawano H, Sagawa K, Koda Y
  • Journal Title: Transfusion Volume: 50 Pages: 1322-1327
  • Peer Reviewed
• [Journal Article] Development and validation of a SYBR Green I-based real-time polymerase chain reaction method for detection of haptoglobin gene deletion in clinical materials (2010)
  • Author(s): Soejima M, Tsuchiya Y, Egashira K, Kawano H, Sagawa K, Koda Y.
  • Journal Title: Transfusion. (In press)
  • Peer Reviewed
• [Journal Article] Global analysis of genetic variation in human arsenic(+3 oxidation state)methyltransferase(AS3MT) (2009)
  • Author(s): Fujihara J, Soejima M, Yasuda T, Koda Y, Agusa T, Kunito T, Tongu M, Yamada T, Takeshita H.
  • Journal Title: Toxicol Appl Pharmacol. 243 Pages: 292-299
  • Peer Reviewed
• [Journal Article] Haptoglobin polymorphism in Mongolian population : comparison of the two genotyping methods (2009)
  • Author(s): Nakamura H, Soejima M, Munkhtulga L, Iwamoto S, Koda Y.
  • Journal Title: Clin Chim Acta. 191 Pages: 236-239
  • Peer Reviewed
• [Journal Article] Simple and sensitive method for identification of human DNA by allele-specific polymerase chain reaction of FOXP2 (2009)
  • Author(s): Hiroshige K, Soejima M, Nishioka T, Kamimura S, Koda Y.
  • Journal Title: J Forensic Sci. 54 Pages: 857-861
  • Peer Reviewed
• [Journal Article] Genetic variation of FUT3 in Ghanaians, Caucasians, and Mongolians (2009)
  • Author(s): Soejima M, Munkhtulga L, Iwamoto S, Koda Y.
  • Journal Title: Transfusion 49 Pages: 959-966
  • Peer Reviewed
• [Presentation] FOXP2を用いた人獣鑑別法 (2011)
  • Author(s): 副島美貴子, 廣重憲一, 吉本譲爾, 神田芳郎
  • Organizer: 日本DNA多型学会第20回学術集会
  • Place of Presentation: はまぎんホールヴィアマーレ(神奈川県)
  • Year and Date: 2011-12-01
• [Presentation] 心筋に広範な収縮帯壊死を認めた小児虐待の一剖検例 (2011)
  • Author(s): 神田芳郎, 副島美貴子
  • Organizer: 第61回日本法医学会学術九州地方集会
  • Place of Presentation: 長崎大学医学部良順会館ボードウィンホール(長崎県)
  • Year and Date: 2011-10-07
• [Presentation] FOXP2を用いたヒトDNAの選択的定量法 (2011)
  • Author(s): 副島美貴子, 神田芳郎
  • Organizer: 第61回日本法医学会学術九州地方集会
  • Place of Presentation: 長崎大学医学部良順会館ボードウィンホール(長崎県)
  • Year and Date: 2011-10-07
• [Presentation] Simple method for identification of human DNA by real-time PCR of FOXP2 (2011)
  • Author(s): Koda Y, Soejima M, Hiroshige K
  • Organizer: 8th International Symposium ADVANCES IN LEGAL MEDICINE
  • Place of Presentation: Johann Wolfgang Goethe-University (Frankfurt, Germany)
  • Year and Date: 2011-09-27
• [Presentation] LAMP法を用いた輸血副作用原因遺伝子ハプトグロビン欠失アリルの検出 (2011)
  • Author(s): 副島美貴子, 神田芳郎
  • Organizer: 第95次日本法医学会総会
  • Place of Presentation: コラッセ福島(福島県)
  • Year and Date: 2011-06-17
• [Presentation] Delta-aminolevulinic acid dehydratase (ALAD) G177C (Asn59Lys)多型の頻度分布における民族差異 (2011)
  • Author(s): 藤原純子, 竹下治男, 安田年博, 加藤秀章, 神田芳郎, 副島美貴子
  • Organizer: 第95次日本法医学会総会
  • Place of Presentation: コラッセ福島(福島県)
  • Year and Date: 2011-06-17
• [Presentation] ヒ素代謝に関与するAS3MT遺伝子多型の民族差異 (2011)
  • Author(s): 藤原純子, 竹下治男, 安田年博, 加藤秀章, 神田芳郎, 副島美貴子
  • Organizer: 第95次日本法医学会総会
  • Place of Presentation: コラッセ福島(福島県)
  • Year and Date: 2011-06-16
• [Presentation] LAMP法を用いた輸血副作用原因遺伝子Hp^<del>の検出法 (2011)
  • Author(s): 神田芳郎, 川野洋之, 江頭弘一, 佐川公矯
  • Organizer: 第59回日本輸血・細胞治療学会総会
  • Place of Presentation: (震災により中止)(紙上発表)
  • Year and Date: 2011-04-14
• [Presentation] Y染色体のハプログループを利用した民族推定法の開発 (2010)
  • Author(s): 室友紀, 今村真二, 渡部有希菜, 中村博明, 藤原純子, 飯田礼子, 安田年博, 木村かおり, 湯浅勲, 副島美貴子, 神田芳郎, 竹下治男
  • Organizer: 日本DNA多型学会第19回学術集会
  • Place of Presentation: 三島市(静岡県)
  • Year and Date: 2010-11-18
• [Presentation] TaqMan法を用いたFUT2 CNV検査法を用いた新規Alu mediated deletlonの同定 (2010)
  • Author(s): 副島美貴子, 神田芳郎
  • Organizer: 日本DNA多型学会第19回学術集会
  • Place of Presentation: 三島市(静岡県)
  • Year and Date: 2010-11-18
• [Presentation] 急性出血性膵炎により死亡したと思われる水中死体の-症例 (2010)
  • Author(s): 神田芳郎, 副島美貴子, 上村繁雄
  • Organizer: 第60回日本法医学会学術九州地方集会
  • Place of Presentation: 北九州(福岡県)
  • Year and Date: 2010-10-15
• [Presentation] 輸血副作用原因遺伝子ハプトグロビン欠失アリルの輸血前診断 (2010)
  • Author(s): 副島美貴子, 神田芳郎
  • Organizer: 第94次日本法医学会総会
  • Place of Presentation: 江戸川区(東京都)
  • Year and Date: 2010-06-25
• [Presentation] DNASE IおよびDNase II遺伝子exon内SNP検索:集団調査及び酵素活性に及ぼす変異の影響 (2010)
  • Author(s): 木村かおり, 藤原純子, 竹下治男, 飯田礼子, 安田年博, 副島美貴子, 神田芳郎, 加藤秀章
  • Organizer: 第94次日本法医学会総会
  • Place of Presentation: 江戸川区(東京都)
  • Year and Date: 2010-06-24
• [Presentation] TaqMan法を用いたFUT2 CNV検査法を用いた新規Alu mediated deletionの同定 (2010)
  • Author(s): 副島美貴子, 神田芳郎
  • Organizer: 日本DNA多型学会第19回学術集会抄録集
  • Place of Presentation: 三島
• [Presentation] TaqMan法を用いたFUT2の Copy Number Variation(CNV)検査法の確立 (2009)
  • Author(s): 副島美貴子, 神田芳郎
  • Organizer: 日本DNA多型学会第18回学術集会
  • Place of Presentation: 久留米市
  • Year and Date: 2009-11-20
• [Presentation] CDGの検査で遭遇したトランスフエリンの二つの変異型 (2009)
  • Author(s): 湯浅勲, 松尾宗明, 副島美貴子, 神田芳郎
  • Organizer: 日本DNA多型学会第18回学術集会
  • Place of Presentation: 久留米市
  • Year and Date: 2009-11-20
• [Presentation] Genotyping Method for Common Polymorphisms of Haptoglobin and Distribution of HP^<DEL> in Asia (2009)
  • Author(s): Koda Y, Soejima M.
  • Organizer: XXth Regional Congress of the ISBT, Asia
  • Place of Presentation: 名古屋市
  • Year and Date: 2009-11-16
• [Presentation] ハプトグロビン多型とモンゴル人集団における生活習慣病の相関解析 (2009)
  • Author(s): 神田芳郎, 副島美貴子, 上村繁雄, 岩本禎彦
  • Organizer: 第59回日本法医学会九州地方会
  • Place of Presentation: 佐賀市
  • Year and Date: 2009-10-02
• [Presentation] Real-time PCRを用いたハプトグロビン遺伝子型判定-Hp Johnson の場合- (2009)
  • Author(s): 副島美貴子, 岩本禎彦, 上村繁雄, 神田芳郎
  • Organizer: 第93次日本法医学会総会
  • Place of Presentation: 大阪市
  • Year and Date: 2009-05-14
• [Presentation] DNASE Iexon 内SNP検索:アジア人において DNase I Gln222Argのみが多型性を有する (2009)
  • Author(s): 藤原純子, 高塚尚和, 竹下治男, 神田芳郎, 副島美貴子, 小湊慶彦, 田島裕, 中島たみ子, 飯田礼子, 安田年博
  • Organizer: 第93次日本法医学会総会
  • Place of Presentation: 大阪市
  • Year and Date: 2009-05-14
• [Presentation] TaqMan法を用いたFUT2のCopy Number Variation(CNV)検査法の確立 (2009)
  • Author(s): 副島美貴子, 神田芳郎
  • Organizer: 日本DNA多型学会第18回学術集会抄録集
  • Place of Presentation: 久留米
• [Book] TaqMan法を用いたFUT2 CNV検査法を用いた新規Alu mediated deletionの同定DNA多型(日本DNA多型学会編) (2011)
  • Author(s): 副島美貴子, 神田芳郎
• [Book] DNA多型Vol.19 (2011)
  • Author(s): 副島美貴子, 神田芳郎
  • Total Pages: 305
  • Publisher: 東洋書店
• [Book] TaqMan法を用いたFUT2のCopy Number Variation(CNV)検査法の確立DNA多型(日本DNA多型学会編) (2010)
  • Author(s): 副島美貴子, 神田芳郎
• [Book] DNA多型 Vol.18 (2010)
  • Author(s): 副島美貴子, 神田芳郎
  • Total Pages: 305
  • Publisher: 東洋書店
• [Book] DNA多型 Vol.17 (2009)
  • Author(s): 副島美貴子, 神田芳郎
  • Total Pages: 296
  • Publisher: 東洋書店
• [Remarks]
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• [Remarks]
  • URL: http://www.med.kurume-u.ac.jp/med/foren/
• [Remarks]
  • URL: http://www.med.kurume-u.ac.jp/med/foren/
• [Remarks]
  • URL: http://www.med.kurume-u.ac.jp/med/foren/