The determination of vital reactions in charred bodies by GC-MS analysis of pyrolysis products in blood

• Project/Area Number: 21590751
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Fukuoka University
• Principal Investigator: HARA Kenji 福岡大学, 医学部, 講師 (00090738)
• Co-Investigator(Kenkyū-buntansha): KUBO Shinichi 福岡大学, 医学部, 教授 (10205122) ; KASHIWAGI Masayuki 福岡大学, 医学部, 助教 (70301687)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 焼死体 / 揮発性物質 / 高熱分解産物 / ガスクロマトグラフィー / 質量分析法 / 固相マイクロ抽出 / 生活反応 / 血液 / 熱分解産物 / GC-MS / スチレン / インデン / 焼死体血液の分析 / 生活反応のマーカー / 揮発性炭化水素 / ニトリル誘導体 / 熱分解物 / 法医病理所見 / GC-MS分析 / 血中揮発成分 / 低温気化平衡法 / GCオーブン冷却濃縮

Research Abstract

We studied the analysis of pyrolysis products, or smoke components, from the blood of charred bodies. The analysis method included cryogenic-focusing gas chromatography-mass spectrometry following solid-phase micro-extraction of the volatile compounds from the headspace of a blood sample vial. Using this method, pyrolysis products such as phenylacetylene, styrene, indene, and naphthalene were detected from the blood of charred bodies that had accompanying heat wounds. The smoke components generated from a fire event being found in a charred body's blood could serve as evidence of the charred body having been alive at the start of the fire.

Research Products

• [Presentation] Faster gas chromatography-mass spectrometry analysis using tandem columns (2011)
  • Author(s): Hara, K., Kashiwagi, M., Waters, B., Matsusue, A., Sugimura, T., Hamasato, S., Kubo, S.
  • Organizer: 8th International Symposium Advances in Legal Medicine(ISALM)
  • Place of Presentation: Frankfurt am Main, Germany
• [Presentation] Simple and practical sample preparation for detection of triazolam in body material by gas chromatography-negative-ion chemical ionization mass spectrometry. (2010)
  • Author(s): 原健二、久保真一、柏木正之
  • Organizer: 48th TIAFT
  • Place of Presentation: Bonn, Germany
  • Year and Date: 2010-08-31
• [Presentation] Demonstration of pyrolysis products in blood from a charred cadaver by cryogenic gas chromatography-mass spectrometry following low-temperature headspace solid-phase microextraction (2009)
  • Author(s): 原健二
  • Organizer: 47^<th> Annual Meeting of the International Association of Forensic Toxicologiests Conference in Geneva
  • Place of Presentation: ジュネーブ(スイス)
  • Year and Date: 2009-08-23
• [Presentation] Demonstration of pyrolysis products in blood from charred cadaver by cryogenic gas chromatography-mass spectrometry following low-temperature headspace solid-phase microextraction (2009)
  • Author(s): Hara, K., Kubo, S., Kashiwagi, M., Matsusue, A., Kageura, M.
  • Organizer: 47th TIAFT
  • Place of Presentation: Geneva, Switzerland