microRNA alteration under chronic starvation stress

• Project/Area Number: 21590767
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General internal medicine (including Psychosomatic medicine)
• Research Institution: Kyushu University
• Principal Investigator: TAKAKURA Shu 九州大学, 大学病院, 助教 (40532859)
• Co-Investigator(Kenkyū-buntansha): KAWAI Keisuke 九州大学, 大学病院, 講師 (80325521)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: miRNA / タンパク / マイクロアレイ / 神経性食欲不振症 / 長期飢餓ストレス / ターゲットmRNA

Research Abstract

We used 9 week BALB-c mice. We found 40% restriction group was best suited for a model of chronic staving stress model and for further examinations. Microarray analysis using total RNA from mouse hippocampus revealed that expressions of several miRNAs were significantly altered under starvation stress condition compared to control group. In the real-time PCR analysis, miR-7 was significantly up-regulated. One of the putative targets for miR-7 was the mRNA which codes proteins related to neurogenesis and so on. There have been no reports that many miRNA altered under chronic starvation stress. Those findings might contribute to establish a new biomarker of starvation conditions such as anorexia nervosa.

Research Products

• [Presentation] 長期飢餓ストレスによるマウス海馬のマイクロRNA(miRNA)発現異常 (2012)
  • Author(s): 高倉修、河合啓介、瀧井正人、須藤信行
  • Organizer: 第53回日本心身医学会総会
  • Place of Presentation: 鹿児島
  • Year and Date: 2012-05-26
• [Presentation] 長期飢餓ストレスによるマウス海馬のマイクロRNA(miRNA)発現異常 (2012)
  • Author(s): 高倉修
  • Organizer: 日本心身医学会総会
  • Place of Presentation: 鹿児島(発表確定)
  • Year and Date: 2012-05-26
• [Remarks]
  • URL: http://hyoka.ofc.kyushu-u.ac.jp/search/details/K003595/research.html