The role of functional TRP ion channels on esophageal perception

• Project/Area Number: 21590791
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Nagoya City University
• Principal Investigator: KAMIYA Takeshi 名古屋市立大学, 大学院・医学研究科, 准教授 (10254301)
• Co-Investigator(Kenkyū-buntansha): SHIMADA Shoichi 大阪大学, 大学院・医学系研究科, 教授 (20216063)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 食道 / 知覚 / イオンチャンネル / 食道知覚 / TRP

Research Abstract

TRPV4 channels were mainly expressed in the esophageal epithelial cells of the basal and intermediate layers. Both 4α-PDD, a selective agonist for TRPV4, and hypoosmolar solution elevated the intracellular calcium concentration in a subset of the isolated esophageal cells. These calcium increases were inhibited by RuR, a TRPV4 antagonist, and were suppressed by extracellular protons. Direct exposure of TRPV4-expressing cells to gastric acid, as would occur in the case of GERD, could influence their cellular functions, possibly aggravating the disease state.

Research Products

• [Journal Article] Acid inhibits TRPV4-mediated Ca^2+influx in mouse esophageal epithelial cells (2011)
  • Author(s): Shikano M, Ueda T, Kamiya T, Ishida Y, Yamada T, Mizushima T, Shimura T, Mizoshita T, Tanida S, Kataoka H, Shimada S, Ugawa S, Joh T
  • Journal Title: Neurogastroenterol Motil. Volume: 23(11) Issue: 11 Pages: 1020-1028
  • DOI: 10.1111/j.1365-2982.2011.01767.x
  • Peer Reviewed
• [Journal Article] The TRPV4 channel is a novel regulator of intracellular Ca^2+in human esophageal epithelial cells (2011)
  • Author(s): Ueda T, Shikano M, Kamiya T, Joh T, Ugawa S
  • Journal Title: Am J Physiol Gastrointest Liver Physiol. Volume: 301(1) Issue: 1 Pages: 138-147
  • DOI: 10.1152/ajpgi.00511.2010
  • Peer Reviewed
• [Journal Article] マウス食道上皮における酸感受性TRPV4の発現と機能 (2011)
  • Author(s): 神谷武、鹿野美千子、城卓志、植田高史、鵜川眞也、島田昌一
  • Journal Title: Therapeutic Research Volume: 32巻 Pages: 585-589
  • URL: http://www.lifescience.co.jp/
• [Journal Article] FDに知覚異常は関与するか (2011)
  • Author(s): 神谷武、鹿野美千子、城卓志
  • Journal Title: 分子消化器病. Volume: 8巻 Pages: 340-344
  • URL: http://www.sentan.com
• [Journal Article] FDに知覚異常は関与しているか (2011)
  • Author(s): 神谷武
  • Journal Title: 分子消化器病 Volume: 8 Pages: 304-344
• [Presentation] Functional TRPV4 channels are expressed in mouse esophageal epithelial cells (2011)
  • Author(s): 神谷武
  • Organizer: United European Gastroenterology Week 2011欧州消化器病週間
  • Place of Presentation: スウェーデンストックホルム
  • Year and Date: 2011-10-25
• [Presentation] Acid-sensitive TRPV4 channel is expressed in mouse esophageal epithelial cells (2011)
  • Author(s): 神谷武
  • Organizer: Digestive disease Week 2011アメリカ消化器病学会
  • Place of Presentation: アメリカシカゴ
  • Year and Date: 2011-05-09
• [Presentation] マウス食道上皮における酸感受性TRPV4の発現と機能 (2011)
  • Author(s): 鹿野美千子
  • Organizer: 第7回日本消化管学会総会ワークショップ
  • Place of Presentation: 京都 国立京都国際会館
  • Year and Date: 2011-02-19
• [Presentation] Functional TRPV4 channels are expressed in mouse esophageal epithelial cells (2011)
  • Author(s): Kamiya T, Shikano M, Hirata Y, Mizushima T, Murakami K, Shimura T, Mizoshita T, Mori Y, Tanida S, Kataoka H, Joh T
  • Organizer: United European Gastroenterology Week 2011
  • Place of Presentation: Stockholm, Sweden
• [Presentation] Acid-sensitive YRPV4 channel is expressed in mouse esophageal epithelium cells (2011)
  • Author(s): Kamiya T, Shikano M, Hirata Y, Mizushima T, Murakami K, Shimura T, Mizoshita T, Mori Y, Tanida S, Kataoka H, Ueda T, Ugasa S, Ishida Y, Shimada S, Joh T
  • Organizer: Digestive Disease Week 2011
  • Place of Presentation: Chicago, USA
• [Presentation] マウス食道上皮における酸感受性TRPV4の発現と機能 (2011)
  • Author(s): 神谷武、鹿野美千子、海老正秀、田中守、平田慶和、水島隆史、志村貴也、村上賢治、溝下勤、森義徳、谷田諭史、片岡洋望、城卓志
  • Organizer: 第7回日本消化管学会総会ワークショップ
  • Place of Presentation: 京都
• [Presentation] 食道上皮でのTRPV4の発現と機能 (2010)
  • Author(s): 鹿野美千子
  • Organizer: 第96回日本消化器病学会総会
  • Place of Presentation: 新潟 新潟県民会館・新潟市民芸術文化会館・新潟市音楽文化会館・朱鷺メッセ
  • Year and Date: 2010-04-24
• [Presentation] 食道上皮でのTRPV4の発現と機能 (2010)
  • Author(s): 鹿野美千子、神谷武、田中守、海老正秀、水島隆史、平田慶和、志村貴也、村上賢治、溝下勤、久保田英嗣、谷田諭史、片岡洋望、城卓志
  • Organizer: 第96回日本消化器病学会総会
  • Place of Presentation: 新潟
• [Presentation] 食道上皮におけるTRPV1, TRPV4の発現 (2009)
  • Author(s): 神谷武
  • Organizer: 第37回日本潰瘍学会学術集会
  • Place of Presentation: 北里大学薬学部(東京都)
  • Year and Date: 2009-11-07
• [Presentation] 食道上皮におけるTRPV1, TRPV4の発現 (2009)
  • Author(s): 神谷武、鹿野美千子、片岡洋望、谷田諭史、溝下勤
  • Organizer: 第37回日本潰瘍学会
  • Place of Presentation: 東京
• [Remarks] 名古屋市立大学大学院医学研究科消化器代謝内科学ホームページ業績集
  • URL: http://www.ncu-shotai.ac/index.html