Analysis of homing system for bone marrow derived cells into gastric cancer tissues through MCP-1 stimulation

• Project/Area Number: 21590796
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Nippon Medical School
• Principal Investigator: FUTAGAMI Seiji 日本医科大学, 医学部, 助教 (50247011)
• Co-Investigator(Kenkyū-buntansha): SAKAMOTO Choitsu 日本医科大学, 医学部, 教授 (30196092)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: Bone marrow derived cells / MCP-1 / migration / CD133-/CD44-positive cell / gastric cancer / CD44 / CD133 / stem cell / ホーミング / CD133陽性細胞 / CCR2 / H.pylori / 癌幹細胞 / 胃癌

Research Abstract

We conformed that CD133-/CD44-positive cells were migrated into the gastric cancer tissues. In addition, we found that CD133-/CD44-positive cells infiltrated in the ascites of the patients with advanced gastric cancer tissues. MCP-1 significantly inhibited the migration of these cells using migration assay.

Research Products

• [Journal Article] G-proteinβ3 subunit 825CC genotype is associated with postprandial distress syndrome with impaired gastric emptying and with the feeling of hunger in Japanese (2011)
  • Author(s): S himpuku M, Futagami S, Kawagoe T, Nagoya H, Shindo T, Horie A, Kodaka Y, Itoh T, Sakamoto C
  • Journal Title: Neurogastroenterol Motil Volume: 23 Issue: 12 Pages: 1073-1080
  • DOI: 10.1111/j.1365-2982.2011.01781.x
  • Peer Reviewed
• [Journal Article] Pathophysiology of Functional Dyspepsia (2011)
  • Author(s): Futagami S, Shimpuku M, Yin Y, Shindo T, Kodaka Y, Nagoya H, Nakazawa S, Fujimoto M, Izumi N, Ohishi N, Kawagoe T, Horie A, Iwakiri K, Sakamoto C
  • Journal Title: Journal of Nippon Medical School Volume: 78 Issue: 5 Pages: 280-285
  • DOI: 10.1272/jnms.78.280
  • NAID: 130004147108
  • ISSN: 1345-4676, 1347-3409
  • Peer Reviewed
• [Journal Article] The incidence of deep vein thrombosis in Japanese patients undergoing endoscopic submucosal dissection (2011)
  • Author(s): K usunoki M, Miyake K, Shindo T, Ueki N, Kawagoe T, Gudis K, Futagami S, et al
  • Journal Title: Gastrointest Endosc Volume: 74 Issue: 4 Pages: 798-804
  • DOI: 10.1016/j.gie.2011.06.015
  • Peer Reviewed
• [Journal Article] The effects of nizatidine on transient lower esophageal sphincter relaxations (TLESRs) and acid reflux in healthy subjects (2011)
  • Author(s): Iwakiri K, Kawami N, Sano H, Tanaka Y, Umezawa M, Futagami S, et al
  • Journal Title: Journal of Smooth Muscle Research Volume: 47 Issue: 6 Pages: 157-166
  • DOI: 10.1540/jsmr.47.157
  • NAID: 10030121548
  • ISSN: 0916-8737, 1884-8796
  • Peer Reviewed
• [Journal Article] 機能性ディスペプシアの病態生理胃排出能と遺伝子多型 (2011)
  • Author(s): 新福摩弓, 二神生爾, 北村忠弘, 坂本長逸
  • Journal Title: 日本臨床生理学会雑誌 Volume: 41 Pages: 87-93
  • NAID: 10030463313
  • Peer Reviewed
• [Journal Article] The incidence of deep vein thrombosis in Japanese patients und ergoing endoscopic submucosal dissection (2011)
  • Author(s): Kusunoki M, Miyake K, Shindo T, Ueki N, Kawagoe T, Gudis K, Futagami S, et al
  • Journal Title: Gastrointest Endosc Volume: 74 Pages: 798-804
  • Peer Reviewed
• [Journal Article] The effects of nizatidine on transient lower esophageal sphincter relaxations (TLESRs) and acid reflux in healthy subjects (2011)
  • Author(s): Iwakiri K, Kawami N, Sano H, Tanaka Y, Umzawa M, Futagami S, et al, et al
  • Journal Title: J Smooth Muscle Res Volume: 47 Pages: 157-166
  • NAID: 10030121548
  • Peer Reviewed
• [Journal Article] Characteristics of symptomatic reflux episodes in patients with non-erosivereflux disease who have a positive symptom index on proton pump inhibitor therapy (2010)
  • Author(s): Iwakiri K, Sano H, Tanaka Y, Kawami N, Umezawa M, Futagami S, et al
  • Journal Title: Digestion Volume: 82(3) Issue: 3 Pages: 156-61
  • DOI: 10.1159/000309483
  • Peer Reviewed
• [Journal Article] Migration of eosinophils and CCR2-/CD68-double positive cells into the duodenal mucosa of patients with postinfectious functional dyspepsia (2010)
  • Author(s): Futagami S, Shindo T, Kawagoe T, Horie A, Shimpuku M, Gudakamoto C
  • Journal Title: Am J Gastroenterol Volume: 105 Issue: 8 Pages: 1835-1842
  • DOI: 10.1038/ajg.2010.151
  • Peer Reviewed
• [Journal Article] Celecoxib inhibits CD133-positive cell migration via reduction of CCR2 in Helicobacter pylori-infected Mongolian gerbils (2010)
  • Author(s): Futagami S, Hamamoto T, Shimpuku M, Nagoya H, Kawagoe T, Horie A, Shindo T, Gudis K, Sakamoto C
  • Journal Title: Digestion Volume: 81 Issue: 3 Pages: 193-203
  • DOI: 10.1159/000252790
  • Peer Reviewed
• [Journal Article] The prokinetic effect of mosapride citrate combined with omeprazole therapy improves clinical symptoms and gastric emptying in PPI-resistant NERD patients with delayed gastric emptying (2010)
  • Author(s): Futagami S, Iwakiri K, Shindo T, Kawagoe T, Horie A, Shimpuku M, Tanaka Y, Kawami N, Gudis K, Sakamoto C
  • Journal Title: J Gastroenterol Volume: 45 Issue: 4 Pages: 413-421
  • DOI: 10.1007/s00535-009-0173-0
  • NAID: 10027215633
  • Peer Reviewed
• [Journal Article] Migration of eosinophils and CCR2-/CD68-double positive cells into the duodenal mucosa of patients with postinfectious functional dyspepsia. (2010)
  • Author(s): Futagami S, et al.
  • Journal Title: Am J Gastroenterol Volume: 105 Pages: 1835-1842
  • Peer Reviewed
• [Journal Article] Celecoxib inhibits CD133-positive cell migration via reduction of CCR2 in Helicobacter pylori-infected Mongolian gerbils. (2010)
  • Author(s): Futagami S, et al.
  • Journal Title: Digestion Volume: 81 Pages: 193-204
  • Peer Reviewed
• [Journal Article] The prokinetic effect of mosapride citrate combined with omeprazole the rapy improves clinical symptoms and gastric emptying in PPI-resistant NERD patients with delayed gastric emptying. (2010)
  • Author(s): Futagami S, et al.
  • Journal Title: J of Gastroenterology Volume: 45 Pages: 413-421
  • Peer Reviewed
• [Journal Article] Celecoxib inhibits CD133-positive cell migration via reduction of CCR2 in Helicobacter pylori-infected Mongolian gerbils. (2010)
  • Author(s): Futagami S, Hamamoto T, Shimpuku M, Nagoya H, Kawagoe T, Horie A, Shindo T, Gudis K, Sakamoto C.
  • Journal Title: Digestion 81 Pages: 193-203
  • Peer Reviewed
• [Journal Article] The prokinetic effect of mosapride citrate combined with omeprazole therapy improves clinical symptoms and gastric PPI-r esistant NERD patients with delayed gastric emptying. (2010)
  • Author(s): Futagami S, Iwakiri K, Shindo T, Kawagoe T, Horie A, Shimpuku M, Tanaka Y, Kawami N, Gudis K, Sakamoto C
  • Journal Title: J Gastroenterol 45 Pages: 413-421
  • Peer Reviewed
• [Journal Article] An analysis of persistent symptoms in acid-suppressed patients with non-erosive reflux disease undergoing esophageal pH-impedance monitoring. (2010)
  • Author(s): Iwakiri K, Sano H, Tanaka Y, Kawami N, Umezawa M, Iizumi T, Futagami S, Hoshihara Y, Nomura T, Miyashita M, Sakamoto C.
  • Journal Title: Nippon Shokakibyo Gakkai Zasshi 107 Pages: 538-548
  • NAID: 10026416955
  • Peer Reviewed
• [Journal Article] Duodenogastric reflux induced by endoscopic submucosal dissection. (2009)
  • Author(s): Miyake K, Kusunoki M, Shindo T, Ueki N, Kawagoe T, Gudis K, Tatsuguchi A, Futagami S, Tsukui T, Sakamoto C.
  • Journal Title: Endoscopy 41 Pages: 934-940
  • Peer Reviewed
• [Journal Article] Impact of quadruple regimen of clarithromycin added to metronidazole-containing triple therapy against Helicobacter infection following clarithromycin-containing triple-therapy failure. (2009)
  • Author(s): Ueki N, Miyake K, Kusunoki M, Shindo T, Kawagoe T, Futagami S, Tsukui T, Inagaki H, Sakamoto C.
  • Journal Title: Helicobacter 14 Pages: 91-99
  • Peer Reviewed
• [Journal Article] Nizatidine improves clinical symptoms and gastric emptying in the patients with functional dyspepsia accompanying with impaired gastric emptying
  • Author(s): Futagami S, et al
  • Journal Title: Digestion Volume: (in press)
  • Peer Reviewed
• [Journal Article] Nizatidine improves clinical symptoms and gastric emptying in the patients with functional dyspepsia accompanying with impaired gastric emptying
  • Author(s): Futagami S, et al
  • Journal Title: Digestion Volume: (In press)
  • Peer Reviewed
• [Presentation] FD患者における随伴症状としての胃食道逆流症状、睡眠障害と胃排出能との相関関係の解析 (2012)
  • Author(s): 泉日輝、二神生爾, 他
  • Organizer: 日本消化器病学会
  • Place of Presentation: 博多
  • Year and Date: 2012-10-20
• [Presentation] 食道癌組織におけるApurinic/apyrimidinic endonuclease-1発現意義の解析 (2012)
  • Author(s): 名児耶浩幸、二神生爾、河越哲郎、小高康裕、新福摩弓, 他
  • Organizer: 第8回日本消化管学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2012-02-10
• [Presentation] 機能性ディスペプシア患者における空腹感スコアとghrelin, preproghrelinの遺伝子多型との相関関係の解析 (2012)
  • Author(s): 二神生爾、新福摩弓、坂本長逸
  • Organizer: 第8回日本消化管学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2012-02-10
• [Presentation] 機能性ディスペプシア患者における空腹感スコアとghrelin, preproghrelinの遺伝子多型との相関関係の検討 (2012)
  • Author(s): 二神生爾, 他
  • Organizer: 第8回日本消化管学会
  • Place of Presentation: 仙台
  • Year and Date: 2012-02-10
• [Presentation] 食道癌組織におけるapurinic/apyrimidinic endonuclease-1(APE-1)発現の解析 (2011)
  • Author(s): 名児耶浩幸、二神生爾、河越哲郎、小高康裕、新福摩弓, 他
  • Organizer: 第97回日本消化器病学会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-14
• [Presentation] H. pylori陽性胃炎患者に対するCOX-2遺伝子多型を用いたハイリスク群の絞り込みの検討 (2011)
  • Author(s): 小高康裕、二神生爾、河越哲郎、名児耶浩幸、新福摩弓, 他
  • Organizer: 第97回日本消化器病学会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-14
• [Presentation] 感染後FD患者における十二指腸粘膜局所の炎症細胞浸潤と胃排出能の解析 (2011)
  • Author(s): 二神生爾、新福摩弓、坂本長逸
  • Organizer: 第97回日本消化器病学会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-14
• [Presentation] Nizatidine administration improves clinical symptoms and gastric emptying of the patients with functional dyspepsia accompanying with impaired gastric emptying (2011)
  • Author(s): Futagami S, Shimpuku M, Kodaka Y, et al.
  • Organizer: Digestive Disease Week
  • Place of Presentation: Chicago, USA
  • Year and Date: 2011-05-07
• [Presentation] Migration of eosinophils and CCR2-/CD68-double positive cells into the duodenal mucosa of patients with post-infectious functional dyspepsia (2011)
  • Author(s): Futagami S, Shimpuku M, Kawagoe T, Kusunoki M, et al
  • Organizer: Digestive Disease Week
  • Place of Presentation: Chicago, USA
  • Year and Date: 2011-05-07
• [Presentation] Celecoxib inhibits CD133-positive cell migration via reduction of CCR2 in Helicobacter pylori-infected Mongolian gerbils (2011)
  • Author(s): Kodaka Y, Futagami S, et al
  • Organizer: Digestive Disease Week
  • Place of Presentation: Chicago, USA
• [Presentation] H. pylori胃癌モデルおよびヒト胃癌組織におけるCD133陽性細胞発現の検討 (2010)
  • Author(s): 二神生爾, 他
  • Organizer: 第16回日本ヘリコバクター学会
  • Place of Presentation: 京都
  • Year and Date: 2010-06-24
• [Presentation] H.pylori感染胃癌モデルおよびヒト胃癌組織におけるCD133陽性細胞発現の検討 (2010)
  • Author(s): 二神生爾, 他
  • Organizer: ヘリコバクター学会
  • Place of Presentation: 京都
  • Year and Date: 2010-06-24
• [Presentation] クロスオーバー法を用いたニザチジンによるFD患者に対する臨床症状と胃排出能への影響の解析- (2010)
  • Author(s): 二神生爾、新福摩弓、河越哲郎、進藤智隆、楠正典、三宅一昌、岩切勝彦、坂本長逸
  • Organizer: 第6回日本消化管学会総会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2010-02-19
• [Presentation] 胃粘膜障害修復過程の解析-MCP-1刺激とCOX-2発現- (2009)
  • Author(s): 二神生爾、辰口篤志、河越哲郎、新福摩弓、進藤智隆、三宅一昌、坂本長逸
  • Organizer: 第39回日本創傷治癒学会
  • Place of Presentation: サピアタワー (東京)
  • Year and Date: 2009-12-09
• [Presentation] 選択的COX-2阻害剤celecoxibを用いたH.pylori感染胃癌モデルにおけるAPE-1および胃癌発生抑制の検討 (2009)
  • Author(s): 二神生爾、河越哲郎、坂本長逸
  • Organizer: 第95回日本消化器病学会総会
  • Place of Presentation: 北海道厚生年金会館 (札幌)
  • Year and Date: 2009-05-07
• [Book] FD診察Q & A (2011)
  • Author(s): 新福摩弓、二神生爾、坂本長逸
  • Total Pages: 160
  • Publisher: 日本医事新報社
• [Book] 症候群ハンドブック (2011)
  • Author(s): 二神生爾
  • Total Pages: 757
  • Publisher: 中山書店
• [Book] FD診察Q&A (2011)
  • Author(s): 新福摩弓, 二神生爾, 坂本長逸
  • Publisher: 日本医事新報社