Identification of DNA methylation changes in esophageal cancer before/after chemoradiation therapy using an MCA-microarray and bisulfate pyrosequencing
| Project/Area Number |
21590797
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
ITOH Fumio 聖マリアンナ医科大学, 医学部, 教授 (90223180)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 食道癌 / 治療 / 化学療法 / 効果予測 / 網羅的メチル化解析 / 科学療法 |
|---|
| Research Abstract |
Tumor and adjacent normal tissue biopsy specimens were obtained before/after treatment from 34 patients(124 samples) treated with a uniform CRT protocol. We analyzed genomewide DNA methylation of 12 non-treated test samples(T and matched ADJ : 6 each) by methylated CpG island amplification and microarray(MCAM) method, and selected candidate genes that were highly methylated in T compared to ADJ. CYP26C1 was selected by unsupervised hierarchical clustering after MCAM in test samples, and was also ranked in the top 5 of both molecular & cellular function networks as a drug metabolism category. CYP26C1 DNA methylation was significantly higher in T than ADJ in both test and validation samples. CYP26C1 has a CpG island in promoter area, which is highly methylated in T of ESCC patients.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Prospective study of definitive chemoradiotherapy with S-1 and nedaplatin in patients with stageII/III(non-T4) esophageal cancer2011
Author(s)
Tsuda T, Inaba H, Miyazaki A, Izawa N, Hirakawa M, Watanabe Y, Kitajima S, Hoshikawa Y, Gomi H, Kimura M, Itoh F
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Journal Title
Esophagus
Volume: 8
Pages: 45-51
Related Report
Peer Reviewed
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[Journal Article] Frequent alteration of MLL3 frameshift mutations in microsatellite deficient colorectal cancer2011
Author(s)
Watanabe Y, Castoro RJ, Kim HS, North B, Oikawa R, Hiraishi T, Ahmed SS, Chung W, Cho M-Y, Toyota M, Itoh F, Estecio M R. H., Shen L, Jelinek J, Issa J-P J
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Gastric wash-based molecular testing for antibiotic resistance in helicobacter pylori2011
Author(s)
Baba S, Oishi Y, Watanabe Y, Oikawa R, Morita R, Yoshida Y, Hiraishi T, Maehata T, Nagase Y, Fukuda Y, Nakazawa M, Ishigouoka S, Hattori N, Suzuki H, Toyota M, Niwa H, Suzuki M, Itoh F
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Journal Title
Digestion
Volume: 84
Pages: 299-305
Related Report
Peer Reviewed
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[Journal Article] Hypermethylation of Sox17 gene is useful as a molecular diagnostic application in early gastric cancer
Author(s)
Oishi Y, Watanabe Y, Yoshida Y, Sato Y, Hiraishi T, Oikawa R, Maehata T, Suzuki H, Toyota M, Niwa H, Suzuki M, Itoh F
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Journal Title
Tumour Biol
Volume: (in press)
Related Report
Peer Reviewed
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