Transient receptor potential A1 mediates gastric distention-induced visceral pain in rats

• Project/Area Number: 21590798
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Hyogo Medical University
• Principal Investigator: TANAKA Junji 兵庫医科大学, 医学部, 助教 (90412022)
• Co-Investigator(Kenkyū-buntansha): MIYOSHI Kan 兵庫医科大学, 医学部, 助教 (30454755)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 上部消化管学(食道、胃、十二指腸) / 内蔵痛 / TAPA1 / 後根神経節 / 迷走神経下神経節 / 内臓痛 / 胃 / 疼痛 / TRPA1

Research Abstract

TRPA1 was predominantly expressed with sensory neuropeptides in dorsal root ganglion(DRG) and nodose ganglion(NG) neurons, and in nerve fibers in the rat stomach. Intrathecal injection of TRPA1 antisense attenuated the visceromotor response, and suppressed ERK1/2 activation in the DRG, but not NG, neurons produced by gastric distention(GD). Furthermore, intrathecal and intraperitoneal injections of the TRPA1 inhibitor HC-03003 suppressed the response to noxious GD. The activation of TRPA1 in DRG neurons by noxious GD may be involved in acute visceral pain.

Research Products

• [Journal Article] Re-evaluation of the phenotypic changes in L4 dorsal root ganglion neurons after L5 spinal nerve ligation (2012)
  • Author(s): Fukuoka T, Yamanaka H, Kobayashi K, Okubo M, Miyoshi K, Dai Y, Noguchi K
  • Journal Title: Pain Volume: 153 Issue: 1 Pages: 68-79
  • DOI: 10.1016/j.pain.2011.09.009
  • Peer Reviewed
• [Journal Article] Spinal 5-HT(3) receptor activation induces behavioral hypersensitivity via a neuronal-glial-neuronal signaling cascade (2011)
  • Author(s): Gu M, Miyoshi K, Dubner R, Guo W, Zou S, Ren K, Noguchi K, Wei F.
  • Journal Title: J. Neurosci. Volume: 31 Issue: 36 Pages: 12823-12836
  • DOI: 10.1523/jneurosci.1564-11.2011
  • Peer Reviewed
• [Journal Article] Sleep disturbances in a neuropathic pain-like condition in the mouse are associated with altered GABAergic transmission in the cingulate cortex (2011)
  • Author(s): Narita M, Niikura K, Nanjo-Niikura K, Narita M, Furuya M, Yamashita A, Saeki M, Matsushima Y, Imai S, Shimizu T, Asato M, Kuzumaki N, Okutsu D, Miyoshi K, Suzuki M, Tsukiyama Y, Konno M, Yomiya K, Matoba M, Suzuki T.
  • Journal Title: Pain Volume: 152 Issue: 6 Pages: 1358-1372
  • DOI: 10.1016/j.pain.2011.02.016
  • Peer Reviewed
• [Journal Article] Serotonin transporter gene polymorphism may be associated with functional dyspepsia in a Japanese population (2011)
  • Author(s): Toyoshima F, Oshima T, Nakajima S, Sakurai J, Tanaka J, Tomita T, Hori K, Matsumoto T, Miwa H.
  • Journal Title: BMC Med Genet Volume: 12 Issue: 1 Pages: 12-88
  • DOI: 10.1186/1471-2350-12-88
  • Peer Reviewed
• [Journal Article] The G-Protein beta 3 subunit 825 TT genotype is associated with epigastric pain syndrome-like dyspepsia (2010)
  • Author(s): Oshima T, et.al.
  • Journal Title: BMC Med Genet Volume: 11 Pages: 13-13
  • Peer Reviewed
• [Journal Article] Transient receptor potential A1 mediates gastric distention-induced visceral pain in rats (2009)
  • Author(s): Kondo T., et.al.
  • Journal Title: Gut 58 Pages: 1342-1352
  • Peer Reviewed
• [Presentation] Activation of p38 MAPK through transient receptor potential A1 mediates gastric distention-in-duced visceral pain in rats (2011)
  • Author(s): Kondo T, Oshima T, Yamasaki T, Okugawa T, Asano H, Toyoshima F, Sakurai J, Tanaka J, Tomita T, Fukui H, Hori K, Watari J, Matsumoto T, Noguchi K, Miwa H.
  • Organizer: 19th United European Gastroenterology Week(UEGW2011)
  • Place of Presentation: Stockholm
  • Year and Date: 2011-10-22
• [Presentation] S-1/Lentinan+Paclitaxel併用療法により良好なQOLを維持し長期生存が得られている癌性腹水を伴う切除不能進行胃癌の1例 (2010)
  • Author(s): 田中淳二, 他14名
  • Organizer: 日本消化器病学会近畿支部第93回例会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-18
• [Presentation] Critical role for spinal IL-18/caspase-1 interaction in neuropathic pain development through its distinct regulation of interleukins (2010)
  • Author(s): Miyoshi K, Noguchi K.
  • Organizer: World Congress on PAIN
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2010-08-30
• [Presentation] 脊髄ミクログリアにおけるcaspase-1の発現増加は炎症性サイトカインの活性化を介して神経障害性疼痛の発症に関与する (2009)
  • Author(s): 三好歓, 他1名
  • Organizer: 第2回日本運動器疼痛研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-07
• [Presentation] Interleukin-1β converting enzyme(ICE)upregulation in spinal glial cells contributes to tactile allodynia after nerve injury (2009)
  • Author(s): Miyoshi K., et.al.
  • Organizer: The 39th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Chicago
  • Year and Date: 2009-10-20
• [Presentation] 脊髄マイクログリアにおけるinterleukin-1β converting enzyme(ICE)の発現増加とアロディニア (2009)
  • Author(s): 三好歓, 他2名
  • Organizer: 第31回日本疼痛学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-07-17
• [Presentation] 脊髄マイクログリアにおけるICEの発現増加とアロディニア (2009)
  • Author(s): 三好歓, 他2名
  • Organizer: 第7回整形外科痛みを語る会
  • Place of Presentation: 福岡
  • Year and Date: 2009-07-04
• [Book] 第6章6胃癌-消化器内科学 (2010)
  • Author(s): 田中淳二, 三輪洋人
  • Publisher: シュプリンガ-ジャパン
• [Book] 第vii章消化器系の症状・徴候と疾患3-消化関係の症状・徴候G.胃・十二指腸潰瘍-疾病と治療ii (2010)
  • Author(s): 田中淳二
  • Publisher: 南江堂