Mechanisms of abdominal symptom development-involvement of neural inflammation and mucosal permeability on esophageal symptoms-

• Project/Area Number: 21590799
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Hyogo Medical University
• Principal Investigator: MIWA Hiroto 兵庫医科大学, 医学部, 教授 (80190833)
• Co-Investigator(Kenkyū-buntansha): OSHIMA Tadayuki 兵庫医科大学, 医学部, 講師 (00381814) ; SAKURAI Jun 兵庫医科大学, 医学部, 助教 (30528998)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 上部消化管学(食道,胃,十二指腸) / 逆流性食道炎 / ニューロキニン / サブスタンスP

Research Abstract

Although the factors which were involved in the development of abdonominal symptoms had not been clear, we found that neuropeptides and those receptors were involved in the development of abdominal symptoms using rat esophagitis model. Furthermore, we established esophageal like stratified epithelial cell layer model and found that superficial layers were important for the epithelial barrier function and that claudin-4 was involved in the barrier function.

Research Products

• [Journal Article] Acid modulates the squamous epithelial barrier function by modulating the localization of claudins in the superficial layers (2012)
  • Author(s): Oshima T, Miwa H, et al(5名5番目)
  • Journal Title: Lab Invest Volume: 92 Issue: 1 Pages: 22-31
  • DOI: 10.1038/labinvest.2011.139
  • Peer Reviewed
• [Journal Article] Acidic bile salts modulate the squamous epithelial barrier function bymodulating tight junction proteins (2011)
  • Author(s): Chen X, Oshima T, Miwa H, et al
  • Journal Title: Am J Physiol Gastrointest Liver Physiol Volume: 301 Issue: 2 Pages: 203-9
  • DOI: 10.1152/ajpgi.00096.2011
  • Peer Reviewed
• [Journal Article] Current understanding of pathogenesis of functional dyspepsia (2011)
  • Author(s): Miwa H, Oshima T, Sakurai J, et al
  • Journal Title: J Gastroenterol Hepatol Volume: Suppl3 Issue: s3 Pages: 53-60
  • DOI: 10.1111/j.1440-1746.2011.06633.x
• [Journal Article] Establishment of esophageal like non-keratinized stratified epithelium using normal human bronchial epithelial cells (2011)
  • Author(s): Oshima T, Miwa H, et al(8名, 7番目)
  • Journal Title: Am J Physiol Cell Physiol Volume: 300 Issue: 6 Pages: 1422-1429
  • DOI: 10.1152/ajpcell.00376.2010
  • Peer Reviewed
• [Journal Article] Establishment of esophageal like non-keratinized stratified epithelium using normal human bronchial epithelial cells (2011)
  • Author(s): Oshima T, Miwa H, et al
  • Journal Title: Am J Physiol Cell Physiol Volume: 300
  • Peer Reviewed
• [Journal Article] Acidic bile salts modulate the squamous epithelial barrier function by modulating tight junction proteins (2011)
  • Author(s): Chen X, Oshima T, Miwa H, et al
  • Journal Title: Am J Physiol Gastrointest Liver Physiol Volume: 301
  • Peer Reviewed
• [Journal Article] Establishment of esophageal like non-keratinized stratified epithelium using normal human bronchial epithelial cells. (2011)
  • Author(s): Oshima T, Miwa H, et al.
  • Journal Title: Am J Physiol Cell Physiol. Volume: (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Rikkunshito a traditional Japanese medicine, may relieve abdominal symptoms in rats withexperimental esophagitis by improving the barrier function of epithelial cells in esophageal mucosa (2010)
  • Author(s): Miwa H, Koseki J, Oshima T, Kondo T, Tomita T, Watari J, Matsumoto T, Hattori T, Kubota K, Iizuka S
  • Journal Title: J Gastroenterol Volume: 45 Issue: 5 Pages: 478-487
  • DOI: 10.1007/s00535-009-0180-1
  • NAID: 10027215919
  • Peer Reviewed
• [Journal Article] NK-1 receptor is involved in the decreased movement in a rat chronic acid reflux oesophagitis model (2010)
  • Author(s): Oshima T, Koseki J, Sakurai J, Watari J, Matsumoto T, Miwa H
  • Journal Title: Neurogastroenterol Motil Volume: 22 Pages: 579-584
  • DOI: 10.1111/j.1365-2982.2009.01450.x
  • Peer Reviewed
• [Journal Article] NK-1 receptor is involved in the decreased movement in a rat chronic acid reflux oesophagitis model. (2010)
  • Author(s): Oshima T, Koseki J, Sakurai J, Watari J, Matsumoto T, Miwa H
  • Journal Title: Neurogastroenterol Motil. 22 Pages: 579-584
  • Peer Reviewed
• [Journal Article] Rikkunshito, a traditional Japanese medicine, may relieve abdominal symptoms in rats with experimental esophagitis by improving the barrier function of epithelial cells in esophageal mucosa. (2010)
  • Author(s): Miwa H, Koseki J, Oshima T, Kondo T, Tomita T, Watari J, Matsumoto T, Hattori T, Kubota K, Iizuka S
  • Journal Title: J Gastroenterol. 45 Pages: 478-487
  • NAID: 10027215919
  • Peer Reviewed
• [Presentation] Pathophysiology and Treatment of Functional Dyspepsia (2011)
  • Author(s): Oshima T
  • Organizer: 2011 National Intestinal Disease Congress & New Technique Forum of Digestive Endoscopy in Tianjin
  • Place of Presentation: Tianjin, China
  • Year and Date: 2011-11-12
• [Presentation] Pathophysiology and Treatment of Functional Dyspepsia (2011)
  • Author(s): Oshima T
  • Organizer: 2011 National Intestinal Disease Congress & New Technique Forum of Digestive Endoscopy in Tianjin
  • Place of Presentation: Tianjin, China(招待講演)
  • Year and Date: 2011-11-12
• [Presentation] Acid modulates the squamous epithelial barrier function by modulating the localization of claudins (2011)
  • Author(s): Oshima T, Miwa H, et al
  • Organizer: DDW2011
  • Place of Presentation: Chicago, USA
  • Year and Date: 2011-05-09
• [Presentation] Visceral symptoms and dilated intercellular spaces in a rat chronic acid reflux esophagitis model are mediated by increased neuropeptides expression in dorsal root ganglion (2011)
  • Author(s): Oshima T, Sakurai J, Miwa H, et al
  • Organizer: DDW2011
  • Place of Presentation: Chicago, USA
  • Year and Date: 2011-05-09
• [Presentation] Acidic bile salt modulates the squamous epithelial barrier function by modulating tight junction protein (2011)
  • Author(s): Chen X, Oshima T, Miwa H, et al
  • Organizer: DDW2011
  • Place of Presentation: Chicago, USA
  • Year and Date: 2011-05-09
• [Presentation] Establishment of a system measuring liquid meal-induced gastric accommodation in conscious guinea pigs (2011)
  • Author(s): Okugawa T, Oshima T, Miwa H, et al
  • Organizer: DDW2011
  • Place of Presentation: Chicago, USA
  • Year and Date: 2011-05-09
• [Presentation] Current understanding of the pathogenesis of functional dyspepsia (2011)
  • Author(s): Miwa H
  • Organizer: Sino-Japan(Tianjin) Symposium of Digestive Diseases
  • Place of Presentation: Tianjin, China
  • Year and Date: 2011-03-06
• [Presentation] Acidic bile salts modulates the squamous epithelial barrier function by modulating tight junction proteins (2010)
  • Author(s): Chen X, Oshima T, Miwa H, et al
  • Organizer: 第38回日本潰瘍学会
  • Place of Presentation: 大阪
  • Year and Date: 2010-11-20
• [Presentation] Bile acids in acidic condition modulate the squamous epithelial barrier function (2010)
  • Author(s): Oshima T, Miwa H, et al
  • Organizer: The 12 World Congress of the International Society for Diseases of the Esophagus(ISDE2010)
  • Place of Presentation: Kagoshima
  • Year and Date: 2010-09-05
• [Presentation] Bile acids in acidic condition modulate the squamous epithelial barrier function (2010)
  • Author(s): Oshima T, Miwa H, et al.
  • Organizer: The 12 World Congress of the International Society for Diseases of the Esophagus (ISDE2010)
  • Place of Presentation: Kagoshima
  • Year and Date: 2010-09-05
• [Presentation] Acid Modulates the Squamous Epithelial Barrier Function by Modulating the Localization of Claudins (2010)
  • Author(s): Oshima T, Miwa H, et al
  • Organizer: Digestive Disease Week 2010
  • Place of Presentation: New Orleans
  • Year and Date: 2010-05-05
• [Presentation] NK-1 receptor is involved in the development of chronic dyspeptic symptom in a rat chronic acid reflux esophagitis model (2009)
  • Author(s): Oshima T, Koseki J, Sakurai J, Matsumoto T, Miwa H
  • Organizer: Digestive Disease Week 2009
  • Place of Presentation: Chicago
  • Year and Date: 2009-06-01
• [Presentation] NK-1 receptor is involved in the development of chronic dyspeptic symptorn in a rat chronic acid reflux esonhagitis model (2009)
  • Author(s): Oshima T, Koseki J, Sakurai J, Matsumoto T, Miwa H
  • Organizer: Digestive Disease Week 2009
  • Place of Presentation: Chicago
  • Year and Date: 2009-06-01
• [Presentation] Involvement of NK1 receptor in the development of chronic dyspeptic symptoms in a rat chronic acid reflux esophagitis model (2009)
  • Author(s): Oshima T, Koseki J, Sakurai J, Tomita T, Matsumoto T, Miwa H
  • Organizer: The 1st Biennial Congress of the Asian Neurogastroenterology and Motility Association
  • Place of Presentation: Seoul
  • Year and Date: 2009-04-04
• [Presentation] Involvement of NK1 receptor in the development of chronic dyspeptic symptoms in a rat chronic acid reflux esophagitis model (2009)
  • Author(s): Oshima T, Koseki J, Sakurai J, Tomita T, Matsumoto T, Miwa H
  • Organizer: The 1st Biennial Congress of the Asian Neurogas troenterology and Motility Association
  • Place of Presentation: Seoul
  • Year and Date: 2009-04-04