Study of interaction between HCV NS3 protein and host protein

• Project/Area Number: 21590824
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Yamagata University
• Principal Investigator: SAITO Takafumi 山形大学, 医学部, 准教授 (80250918)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: C型肝炎 / NS3 / HCV / 蛋白質 / 肝がん / インターフェロン / ウイルス / 二次構造 / 治療 / 予測因子 / 肝細胞がん / HCC / ペグインターフェロン / リバビリン / SVR

Research Abstract

The secondary structure of the 120 residues of the amino-terminal HCV-1b non-structural region 3 (NS3) deduced from the amino acid sequence was classified into three major groups : A, B and C. The association between this polymorphism and virological response to pegylated interferon plus ribavirin therapy to chronic hepatitis C, or an increased risk of development of hepatocellular carcinoma (HCC), was analyzed. The results suggest that polymorphism in the secondary structure of the HCV-1b NS3 amino-terminal region influences the virological response to pegylated interferon plus ribavirin therapy and an increased risk of HCC, and that virus grouping based on this polymorphism can contribute to prediction of the outcome of chronic HCV infection.

Research Products

• [Journal Article] Potential therapeutic application of intravenous bone marrow infusion in patients with alcoholic liver cirrhosis (2011)
  • Author(s): Saito T, Okumoto K, Haga H, Nishise Y, Ishii R, Sato C, Watanabe H, Okada A, Ikeda M, Togashi H, Ishikawa T, Terai S, Sakaida I, Kawata S
  • Journal Title: Stem Cells Dev Volume: 20 Issue: 6 Pages: 1503-1510
  • DOI: 10.1111/j.1348-0421.2011.00331.x
  • Peer Reviewed
• [Journal Article] Serum metabolomics reveals γ-glutamyl dipeptides as biomarkers for discrimination among different forms of liver disease (2011)
  • Author(s): Soga T, Sugimoto M, Honma M, Mori M, Igarashi K, Kashikura K, Ikeda S, Hirayama A, Yamamoto T, Yoshida H, Otsuka M, Tsuji S, Yatomi Y, Sakuragawa T, Watanabe H, Nihei K, Saito T, Kawata S, Suzuki H, Tomita M, Suematsu M
  • Journal Title: J Hepatol Volume: 55 Pages: 896-905
  • Peer Reviewed
• [Journal Article] Potential therapeutic application of intravenous bone marrow in fusion in patients with alcoholic liver cirrhosis (2011)
  • Author(s): Saito T, Okumoto K, et al.
  • Journal Title: Stem Cells Dev Volume: 20 Issue: 7 Pages: 1503-1510
  • DOI: 10.1007/s12149-011-0484-0
  • Peer Reviewed
• [Journal Article] Secondary structure of the amino-terminal region of HCV NS3 and virological response to pegylated interferon plus ribavirin therapy for chronic hepatitis C (2010)
  • Author(s): Sanjo M, Saito T, Ishii R, Nishise Y, Haga H, Okumoto K, Ito J, Watanabe H, Saito K, Togashi H, Fukuda K, Imai Y, El-Shamy A, Deng L, Shoji I, Hotta H, Kawata S
  • Journal Title: J Med Virol Volume: 82 Issue: 8 Pages: 1364-1370
  • DOI: 10.1002/jmv.21818
  • Peer Reviewed
• [Journal Article] Relationship between alcohol consumption and serum adiponectin levels : The Takahata Study-A cross-sectional study of a healthy Japanese population (2010)
  • Author(s): Nishise Y, Saito T, et al
  • Journal Title: J Clin Endocrinol Metab Volume: 95 Pages: 3828-3835
  • Peer Reviewed
• [Journal Article] Down-regulation of hepatic stearoyl-CoA desaturase 1 expression by angiotensin II receptor blocker in the obese fa/fa Zucker rat : possible role in amelioration of insulin resistance and hepatic steatosis (2009)
  • Author(s): Yokozawa J, Sasaki T, Saito T, et al.
  • Journal Title: Journal of Gastroenterology 44 Pages: 583-591
  • NAID: 10025175593
  • Peer Reviewed
• [Journal Article] Impact of metabolic syndrome on elevated serum alanine aminot ransferase levels in the Japanese population (2009)
  • Author(s): Saito T, Nishise Y, Makino N, et al.
  • Journal Title: Metabolism 58 Pages: 1067-1075
  • Peer Reviewed
• [Journal Article] HCV NS3蛋白質多型性とC型慢性肝炎の予後・治療効果予測 (2009)
  • Author(s): 斎藤貴史、西瀬雄子、石井里佳, 他
  • Journal Title: 日本消化器病学会雑誌 106 Pages: 502-507
  • NAID: 10025177763
• [Presentation] Hepatocellular Carcinoma and Secondary Structure of Hepatitis C Virus (HCV) NS3 Protein Amino-Terminus among Patients received Peginterferon plus Ribavirin Combination Therapy for Chronic HCV Subtype 1b Infection (2011)
  • Author(s): Nishise Y, Saito T, et al.
  • Organizer: The 62nd Annual Meeting of the American Association for the Study of Liver Disease
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2011-11-06
• [Presentation] 当科における肝硬変の成因別実態 (2011)
  • Author(s): 石井里佳, 斎藤貴史, 冨田恭子, 佐藤智佳子, 芳賀弘明, 奥本和夫, 西瀬雄子, 渡辺久剛, 冨樫整, 河田純男
  • Organizer: 第15回日本肝臓学会大会
  • Place of Presentation: 福岡市マリンメッセ福岡
  • Year and Date: 2011-10-21
• [Presentation] HCV NS3アミノ末端領域蛋白質二次構造の多型性と治療効果予測 (2011)
  • Author(s): 石井里佳, 斎藤貴史, 河田純男, 他
  • Organizer: 第190回日本消化器病学会東北支部例会
  • Place of Presentation: 仙台市仙台国際センター
  • Year and Date: 2011-02-05
• [Presentation] HCV NS3アミノ末端領域蛋白質二次構造の多型性と治療効果予測 (2010)
  • Author(s): 石井里佳、斎藤貴史, 他
  • Organizer: 第190回日本消化器病学会東北支部例会
  • Place of Presentation: 仙台市仙台国際センター
  • Year and Date: 2010-02-05
• [Presentation] HCV NS3蛋白質二次構造の多型性とHCV RNA量およびペグインターフェロン・リバビリン併用療法の効果予測の検討 (2009)
  • Author(s): 斎藤貴史, 三條麻衣, 堀田博, 河田純男, 他
  • Organizer: 第45回日本肝臓学会総会
  • Place of Presentation: 神戸市神戸ポートピアホテル
  • Year and Date: 2009-06-04
• [Presentation] HCV NS3蛋白質二次構造の多型性とHCVRNA量およびペグインターフェロン・リバビリン併用療法の効果予測の検討 (2009)
  • Author(s): 斎藤貴史、三條麻衣、石井里佳, 他
  • Organizer: 第45回日本肝臓学会総会
  • Place of Presentation: 神戸市
  • Year and Date: 2009-06-04