Analysis of nonalcoholic fatty liver using androgen receptor transgenic mouse
| Project/Area Number |
21590828
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Chiba University |
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Principal Investigator |
IMAZEKI Fumio 千葉大学, 大学院・医学研究院腫瘍内科学, 准教授 (40223325)
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| Co-Investigator(Kenkyū-buntansha) |
新井 誠人 千葉大学, 医学部附属病院, 助教 (30396684)
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| Co-Investigator(Renkei-kenkyūsha) |
ARAI Makoto 千葉大学, 医学部・附属病院, 助教 (30396684)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | アンドロジェン受容体トランスジェニックマウス / 脂質 / シグナル伝達 / アンドロジェン受容体 / トランスジェニックウス / 非アルコール性脂肪性肝炎 / トランスジェニックマウス |
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| Research Abstract |
We had planned to examine the relationship between androgen receptor and lipid or carbohydrate metabolism using androgen receptor transgenic mouse, but the transgenic mouse could not be produced for examination. Two female transgenic mice produced in the first experiment did not raise their children. A total of 139 mice produced in the next 3 consecutive experiments did not have the transgenic genes. Therefore we transfected androgen receptor expression vector into HepG2 and Huh7, and analyzed the change of m-RNA expression in the 84 genes related to lipid metabolism. Among these genes, the only genes down regulated both in the two cells were Acyl-CoA dehydrogenase and Carnitine palmitoyltransferase 1A, both of which are involved in fatty acid oxidation and the result was contrary to our expectation.
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Report
(4 results)
Research Products
(2 results)
