FRET/ BRET analysis of interaction between HCV protein and interferon signaling

• Project/Area Number: 21590831
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: FUJITA Megumi (TASAKA Megumi) 東京医科歯科大学, 大学院・医歯学総合研究科, 特任助教 (50510369)
• Co-Investigator(Kenkyū-buntansha): ASAHINA Yasuhiro 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (00422692) ; AZUMA Seishin 東京医科歯科大学, 医学部附属病院, 講師 (10376783) ; SAKAMOTO Naoya 北海道大学, 医学系研究科, 教授 (10334418)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: HCV レプリコン / インターフェロン / RIG-I / HCV-NS4B / STING / HCVレプリコン / Cardif / 生体発光共鳴エネルギー転移(Bioliminescence Resonance Energy ransfer)

Research Abstract

We investigated protein interaction between Hepatitis C virus (HCV)-NS4B and signaling molecules associated with interferon (IFN) production, including Cardif, and STING. The weak interaction between HCV-NS4B and Cardif was observed. Our data, including BiFC analysis, immunoprecipitation assay, and immunostaining, demonstrated that HCV-NS4B specifically bindsSTING. We showed that NS4B suppresses STING-mediated IFN production.

Research Products

• [Journal Article] Hepatitis C virus NS4B protein targets STING and abrogates RIG-I-mediated type-I interferon-dependent innate immunity (2013)
  • Author(s): Nitta S, Sakamoto N, Nakagawa M, Kakinuma S, Mishima K, Kusano-kitazume A, Kiyohashi K, Murakawa M, Nishimura-Sakurai Y, Azuma S, Tasaka-Fujita M, asahina Y, Yoneyama M, Fujita T, Watanabe M
  • Journal Title: Hepatology Volume: 57 Issue: 1 Pages: 46-58
  • DOI: 10.1002/hep.26017
  • Peer Reviewed
• [Journal Article] Wnt5a Signaling Mediates Biliary Differentiation of Fetal Hepatic Stem/Progenitor Cells (2013)
  • Author(s): Kakinuma S, Kiyohashi K, Kamiya A, Sakamoto N, Nitta S, Yamanaka H, YoshinoK, Fujiki J, Murakawa M, Kusano-Kitazume A, Shimizu H, Okamoto R, Azuma S, Nakagawa M, Asahina Y, Tanimizu N, Kikuchi A, Nakauchi H, and *WatanabeM
  • Journal Title: Hepatology Volume: (in press) Issue: 6 Pages: 2502-2513
  • DOI: 10.1002/hep.26293
  • Peer Reviewed
• [Journal Article] α-fetoprotein levels afterinterferon therapy and risk of hepatocarcinogenesis in chronic hepatitis C (2013)
  • Author(s): Asahina Y, Tsuchiya K, Nishimura T, Muraoka M, Suzuki Y, Tamaki N, Yasui Y, Hosokawa T, Ueda K, Nakanishi H, Itakura J, Takahashi Y, Kurosaki M, Enomoto N, Nakagawa M, Kakinuma S, Watanabe M,Izumi N
  • Journal Title: Hepatology Volume: (in press) Issue: 4 Pages: 1253-1262
  • DOI: 10.1002/hep.26442
  • Peer Reviewed
• [Journal Article] Inhibition of hepatocellular carcinoma by PegIFNα2a in patients with chronic hepatitis C: a nationwide multi-center cooperative study. (2013)
  • Author(s): Izumi N, Asahina Y, Kumada H et al
  • Journal Title: J Gastroenterol Volume: 48 Issue: 3 Pages: 382-390
  • DOI: 10.1007/s00535-012-0641-9
  • Peer Reviewed
• [Journal Article] Noninvasive estimation of fibrosis progression overtime using the FIB-4 index in chronic hepatitis C. (2013)
  • Author(s): Tamaki N, Asahina Y, Izumi N et al
  • Journal Title: J Viral Hepat Volume: 20 Issue: 1 Pages: 72-76
  • DOI: 10.1111/j.1365-2893.2012.01635.x
  • Peer Reviewed
• [Journal Article] Model incorporating the ITPA genotype identifies patients at high risk of anemia and treatment failure with pegylated-interferon plus ribavirin therapy for chronic hepatitis C. (2013)
  • Author(s): Kurosaki M, Sakamoto N, Asahina Y, Izumi N et al
  • Journal Title: J Med Virol Volume: 85 Issue: 3 Pages: 449-458
  • DOI: 10.1002/jmv.23497
  • Peer Reviewed
• [Journal Article] Virological response and safety of 24-week telaprevir alone in Japanese patients infected with hepatitis C virus subtype 1b. (2013)
  • Author(s): Toyota J, Asahina Y, Kumada H et al
  • Journal Title: J Viral Hepat Volume: 20 Issue: 3 Pages: 167-173
  • DOI: 10.1111/j.1365-2893.2012.01640.x
  • Peer Reviewed
• [Journal Article] Impaired brain activity in cirrhotic patients with minimal hepatic encephalopathy: evaluation by near infrared spectroscopy. (2013)
  • Author(s): Nakanishi H,Asahina Y, Izumi N et al
  • Journal Title: Hepatol Res Volume: in press Issue: 3 Pages: 319-326
  • DOI: 10.1111/hepr.12127
  • Peer Reviewed
• [Journal Article] Guidelines for the Management of Hepatitis C Virus Infection. (2013)
  • Author(s): Asahina Y, Hayashi N, Yotsuyanagi H et al
  • Journal Title: Hepatology Research Volume: 43 Pages: 1-34
  • NAID: 130003384472
• [Journal Article] Identification of novel N-(morpholine-4-carbonyloxy) amidine compounds as potent inhibitors against hepatitis C virus replication (2012)
  • Author(s): Kusano-Kitazume A, Sakamoto N, Okuno Y, Sekine-Osajima Y, Nakagawa M, Kakinuma S, Kiyonashi K, Nitta S, Murakawa M, Azuma S, Nishimura-Sakurai Y, Hagiwara M, Watanabe M
  • Journal Title: Antimicrob Agent Chemother Volume: 56(3) Issue: 3 Pages: 1315-23
  • DOI: 10.1128/aac.05764-11
  • Peer Reviewed / Open Access
• [Journal Article] Genome-wide association study confirming association of HLA-DP with protection against chronic hepatitis B and viral clearance in Japanese and Korean. (2012)
  • Author(s): Nishida N, Asahina Y, Mizokami M et al
  • Journal Title: PLoS One Volume: 7 Issue: 6 Pages: 1-8
  • DOI: 10.1371/journal.pone.0039175
  • Peer Reviewed
• [Journal Article] No association for Chinese HBV-related hepatocellular carcinoma susceptibility SNP in other East Asian populations. (2012)
  • Author(s): Sawai H, Asahina Y, Tokunaga K et al
  • Journal Title: BMC Med Genet Volume: 13 Issue: 1 Pages: 47-47
  • DOI: 10.1186/1471-2350-13-47
  • Peer Reviewed
• [Journal Article] Inhibition of hepatitis C virus NS5B polymerase by S-trityl-L-cysteine derivatives (2012)
  • Author(s): Kaushik-Basu N, Sakamoto N
  • Journal Title: Eur J Med Chem Volume: 49 Pages: 191-199
  • DOI: 10.1016/j.ejmech.2012.01.010
  • Peer Reviewed
• [Journal Article] Age and total ribavirin dose are independent predictors of relapse after interferon therapy in chronic hepatitis C revealed by data mining analysis (2012)
  • Author(s): Kurosaki M, Sakamoto N, et al
  • Journal Title: Antivir Ther Volume: 17(1) Pages: 35-43
  • Peer Reviewed
• [Journal Article] Data mining model using simple and readily available factors could identify patients at high risk for hepatocellular carcinoma in chronic hepatitis C (2012)
  • Author(s): Kurosaki M, Sakamoto N, Nakagawa M, et al.
  • Journal Title: J Hepatol Volume: 56(3) Issue: 3 Pages: 602-608
  • DOI: 10.1016/j.jhep.2011.09.011
  • Peer Reviewed
• [Journal Article] Analysis of interferon signaling by infectious hepatitis C virus clones with substitutions of core amino acids 70 and 91 (2011)
  • Author(s): Funaoka Y, Sakamoto N, Suda G, Itsui Y, Nakagawa M, Kakinuma S, Watanabe T, Nitta S, Kitazume A, Kiyohashi K, Murakawa M, Azuma S, Tsuchiya K,Watanabe M
  • Journal Title: J Virol Volume: 85 Issue: 12 Pages: 5986-94
  • DOI: 10.1128/jvi.02583-10
  • Peer Reviewed
• [Journal Article] Inhibitory effect of a triterpenoid compound, with or without alpha interferon, on hepatitis C virus infection (2011)
  • Author(s): Watanabe T, Sakamoto N, Nakagawa M, Azuma S, Watanabe M, et al.
  • Journal Title: Antimicrob Agents Chemother Volume: 55(6) Issue: 6 Pages: 2537-2545
  • DOI: 10.1128/aac.01780-10
  • Peer Reviewed
• [Journal Article] Serum interleukin-6 levels correlate with resistance to treatment of chronic hepatitis C infection withpegylated-interferon-α2b plus ribavirin (2011)
  • Author(s): Ueyama M, Nakagawa M, Sakamoto N,Onozuka I, Funaoka Y, Watanabe T, Nitta S, Kiyohashi K, Kitazume A, Murakawa M, Nishimura-Sakurai Y, Sekine-Osajima Y, Itsui Y, Azuma S, Kakinuma S, Watanabe M and the Ochanomizu-Liver Conference Study Group
  • Journal Title: Antivir Ther Volume: 16 Issue: 7 Pages: 1081-1091
  • DOI: 10.3851/imp1864
  • Peer Reviewed
• [Journal Article] Studies on virus kinetics using infectious fluorescence-tagged hepatitis C virus cell culture (2011)
  • Author(s): Yamamoto M, Sakamoto N, Nakamura T, Itsui Y, Nakagawa M, Nishimura-Sakurai Y, Kakinuma S, Azuma S, Kato T, Wakita T, Watanabe M
  • Journal Title: Hepatol Res Volume: 41 Issue: 3 Pages: 258-269
  • DOI: 10.1111/j.1872-034x.2010.00771.x
  • Peer Reviewed
• [Journal Article] Association of IL28B variants with response to pegylated-interferon alpha plus ribavirin combination therapy reveals intersubgenotypic differences between genotypes 2a and 2b (2011)
  • Author(s): Sakamoto N, Nakagawa M, Azuma S, Watanabe M, et al.
  • Journal Title: J Med Virol Volume: 83 Issue: 5 Pages: 871-878
  • DOI: 10.1002/jmv.22038
  • Peer Reviewed
• [Journal Article] Association of gene expression involving innate immunity and genetic variation in IL28B with antiviral response (2011)
  • Author(s): Asahina Y, Sakamoto N, et al
  • Journal Title: Hepatology Volume: 55(1) Issue: 1 Pages: 20-29
  • DOI: 10.1002/hep.24623
  • Peer Reviewed
• [Journal Article] Analysis of the complete open reading frame of hepatitis C virus in genotype 2a infection reveals critical sites influencing the response to peginterferon and ribavirin therapy (2011)
  • Author(s): Kadokura M, Nakagawa M, Sakamoto N, Watanabe M, et al.
  • Journal Title: Hepatol Int Volume: 5(3) Issue: 3 Pages: 789-799
  • DOI: 10.1007/s12072-011-9267-x
  • Peer Reviewed
• [Journal Article] Use of human hepatocyte-like cells derived from induced pluripotent stem cells as a model for hepatocytes in hepatitis C virus infection (2011)
  • Author(s): Yoshida T, Sakamoto N, et al
  • Journal Title: Biochem Biophys Res Commun Volume: 416(1-2) Issue: 1-2 Pages: 119-124
  • DOI: 10.1016/j.bbrc.2011.11.007
  • Peer Reviewed
• [Journal Article] A new method for induced fit docking (GENIUS) and its application to virtual screening of novel HCV NS3-4A protease inhibitors (2011)
  • Author(s): Takaya D, Sakamoto N, et al.
  • Journal Title: Bioorganic & Medicinal Chemistry Volume: 19(22) Issue: 22 Pages: 6892-6905
  • DOI: 10.1016/j.bmc.2011.09.023
  • Peer Reviewed
• [Journal Article] Serum interleukin-6 levels correlate with resistance to treatment of chronic hepatitis C infection with pegylated-interferon-alpha2b plus ribavirin (2011)
  • Author(s): Ueyama M, Sakamoto N, et al
  • Journal Title: Antivir Ther Volume: 16 Pages: 1081-1091
  • Peer Reviewed
• [Journal Article] Genome-wide association study identified ITPA/DDRGK1 variants reflecting thrombocytopenia in pegylated interferon and ribavirin therapy for chronic hepatitis C (2011)
  • Author(s): Tanaka Y, Sakamoto N, et al.
  • Journal Title: Human Molecular Genetics Volume: 20(17) Issue: 17 Pages: 3507-3516
  • DOI: 10.1093/hmg/ddr249
  • Peer Reviewed
• [Journal Article] Pretreatment Prediction of Anemia Progression by Peginterferon Plus Ribavirin Therapy in Chronic Hepatitis C : Decision-Tree Analysis (2011)
  • Author(s): Hiramatsu N, Sakamoto N, et al.
  • Journal Title: J Gastroenterol Volume: 46(9) Issue: 9 Pages: 1111-1119
  • DOI: 10.1007/s00535-011-0412-z
  • Peer Reviewed
• [Journal Article] Analysis of the complete open reading frame of genotype 2b hepatitis C virus in association with the response to peginterferon and ribavirin therapy (2011)
  • Author(s): Kadokura M, Nakagawa M, Sakamoto N, Watanabe M, et al.
  • Journal Title: PLoS One Volume: 6(9) Issue: 9 Pages: e24514-e24514
  • DOI: 10.1371/journal.pone.0024514
  • Peer Reviewed
• [Journal Article] Pretreatment prediction of response to peginterferon plus ribavirin therapy in genotype 1 chronic hepatitis C using data mining analysis (2011)
  • Author(s): Kurosaki M, Sakamoto N, et al.
  • Journal Title: J Gastroenterol Volume: 46(3) Issue: 3 Pages: 401-409
  • DOI: 10.1007/s00535-010-0322-5
  • NAID: 10029028096
  • Peer Reviewed
• [Journal Article] Adenovirus vector-mediated assay system for hepatitis C virus replication (2011)
  • Author(s): Yoshida T, Sakamoto N, et al
  • Journal Title: Nucleic Acids Res Volume: 39(10) Issue: 10 Pages: e64-e64
  • DOI: 10.1093/nar/gkr047
  • Peer Reviewed
• [Journal Article] Association between lipid accumulation and the cannabinoid system in Huh7 cells expressing HCV genes (2011)
  • Author(s): Toyoda M, Sakamoto N, et al
  • Journal Title: Int J Mol Med Volume: 27 Issue: 5 Pages: 619-624
  • DOI: 10.3892/ijmm.2011.622
  • Peer Reviewed
• [Journal Article] Cholestatic liver fibrosis and toxin-induced fibrosis are exacerbated in matrix metalloproteinase-2 deficient mice (2011)
  • Author(s): Onozuka I, Sakamoto N, Nakagawa M, Watanabe M, et al.
  • Journal Title: Biochem Biophys Res Commun Volume: 406 Issue: 1 Pages: 134-140
  • DOI: 10.1016/j.bbrc.2011.02.012
  • Peer Reviewed
• [Journal Article] Pre-treatment prediction of response to pegylated-interferon plus ribavirin for chronic hepatitis C using genetic polymorphism in IL28B and viral factors (2011)
  • Author(s): Kurosaki M, Sakamoto N, Nakagawa M, Watanabe M, et al.
  • Journal Title: J Hepatol Volume: 54 Issue: 3 Pages: 439-448
  • DOI: 10.1016/j.jhep.2010.07.037
  • Peer Reviewed
• [Journal Article] Sequences in the interferon sensitivity-determining region and core region of hepatitis C virus impact pretreatment prediction of response to PEG-interferon plus ribavirin : data mining analysis (2011)
  • Author(s): Kurosaki M, Sakamoto N, Nakagawa M, et al.
  • Journal Title: J Med Virol Volume: 83 Issue: 3 Pages: 445-452
  • DOI: 10.1002/jmv.22005
  • Peer Reviewed
• [Journal Article] Antiviral effects of the interferon-induced protein guanylate binding protein 1 and its interaction with the hepatitis C virus NS5Bprotein (2009)
  • Author(s): Itsui Y, Sakamoto N, Kakinuma S, Nakagawa M, Sekine-Osajima Y, Tasaka-Fujita M, Nishimura-Sakurai Y, Suda G, Karakama Y, Mishima K, Yamamoto M, Watanabe T, Ueyama M, Funaoka Y, Azuma S, Watanabe M
  • Journal Title: Hepatology Volume: 50(6) Issue: 6 Pages: 1727-37
  • DOI: 10.1002/hep.23195
  • Peer Reviewed
• [Journal Article] HCV-associated gene expression and cell signaling pathway in cells with or without HCV replicon and in replicon-cured cells. (2009)
  • Author(s): Nishimura-Sakurai Y, Sakamoto N, Megumi Tasaka-Fujita
  • Journal Title: J.Gastroentelorogy
  • Peer Reviewed
• [Journal Article] Antiviral effects of the interferon-induced protein GBP-1 and its interaction with the hepatitis C virus NS5B protein (2009)
  • Author(s): Itsui Y, Sakamoto N, Megumi Tasaka- Fujita
  • Journal Title: Hepatology 50 Pages: 1727-1737
  • Peer Reviewed
• [Presentation] Serum interleukin-6 levels during treatment correlate with resistance to telaprevir-based triple therapy in chronic hepatitis C. (2013)
  • Author(s): Asahina Y
  • Organizer: EASL: The international liver congress 2013
  • Place of Presentation: Amsterdam
• [Presentation] HCVgenotype 1b株キメラウイルスを用いた HCV core領域アミノ酸70/91変異株の解析 (2012)
  • Author(s): 藤田めぐみ 、脇田隆字、加藤孝宣
  • Organizer: 第48回日本肝臓学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2012-06-08
• [Presentation] HCV-NS4B protein binds STINGand blocks the RIG-I mediated IFN pathway (2012)
  • Author(s): Sayuri Nitta, Naoya Sakamoto, Mina Nakagawa, Sei Kakinuma, Akiko Kusano Kitazume, Miyako Murakawa, Megumi Tasaka-Fujita, Yasuhiro Asahina, Mamoru Watanabe
  • Organizer: 10th single topic conference
  • Place of Presentation: Tokyo
• [Presentation] HCV-NS4B blocks the RIG-I mediated IFN pathway through targeting of STING (2012)
  • Author(s): Sayuri Nitta, Naoya Sakamoto, Mina Nakagawa, Sei Kakinuma, Kako Mishima, Akiko Kusano-Kitazume, Kei Kiyohashi, Miyako Murakawa, Kohei Yoshino, Megumi Tasaka-Fujita, Yasuhiro Asahina, Mamoru Watanabe
  • Organizer: 19^<th> International Symposium of Hepatitis C Virus and Related Viruses
  • Place of Presentation: Venice, Italy
• [Presentation] HCV NS4B蛋白によるRIG-I誘導性Interferon発現経路の抑制機構についての解析 (2012)
  • Author(s): 新田沙由梨、 坂本直哉 、吉野耕平、村川美也子、北詰晶子、幾世橋佳、東正新、柿沼晴、中川美奈、朝比奈靖浩、渡辺守
  • Organizer: JDDW2012
  • Place of Presentation: 神戸
• [Presentation] IFN不応例における宿主自然免疫応答とHCV逃避機構 (2012)
  • Author(s): 新田沙由梨,朝比奈靖浩,坂本直哉,村川美也子,北詰晶子,幾世橋佳,東正新,柿沼晴,中川美奈,渡辺守
  • Organizer: 第77回日本インターフェロン・サイトカイン学会
  • Place of Presentation: 神戸
• [Presentation] HCVNS4B蛋白はSTINGを介しRIG-I誘導性 Interferon産生応答を抑制する (2012)
  • Author(s): 新田沙由梨、 坂本直哉、吉野耕平、村川美也子、北詰晶子、幾世橋佳、東正新、柿沼晴、中川美奈、 朝比奈靖浩、渡辺守
  • Organizer: 第48回肝臓学会総会
  • Place of Presentation: 金沢
• [Presentation] Supression of Alpha-fetoprotein by Interferon Therapy Reduces The Risk of Hepatocarcinogenesis in Patients with Chronic Hepatitis C. (2012)
  • Author(s): Asahina Y,Tsuchiya K, Izumi N et al
  • Organizer: EASL: The international liver congress 2012
  • Place of Presentation: Barcelona
• [Presentation] Risk factors for exceeding the Milan criteria following successful radiofrequency ablation in patients with early stage hepatocellular carcinoma: determination of patients requiring early transplantation or new adjuvant therapy. (2012)
  • Author(s): Tsuchiya K, Asahina Y, Izumi N et al
  • Organizer: AASLD The Liver Meeting 2012
  • Place of Presentation: Boston
• [Presentation] Reduced organic anion transporter 2 expression is associated with a high risk for hepatocellulare carcinoma in chronic hepatitis C patients: a propensity score-matched study. (2012)
  • Author(s): Yasui Y, Asahina Y, Izumi N et al
  • Organizer: AASLD The Liver Meeting 2012
  • Place of Presentation: Boston
• [Presentation] Imparied IL28B gene induction and poor IL28B promoter activity influenced by the IL28B minor allele are closely associated with a null response to interferon in chronic hepatitis C patients. (2012)
  • Author(s): Asahina Y, Sakamoto N, Azuma S, Watanabe M et al
  • Organizer: AASLD The Liver Meeting 2012
  • Place of Presentation: Boston
• [Presentation] Antiviral effects and action mechanisms of novel N-(morpholine-4-carbonyloxy) amidine compaunds against hepatitis C virus. (2012)
  • Author(s): Kusano-Kitazume A, Sakamoto N, Azuma S, Asahina Y, Watanabe M et al
  • Organizer: AASLD The Liver Meeting 2012
  • Place of Presentation: Boston
• [Presentation] IL-6-mediated intersubgenotypic variation of interferon sensitivity in hepatitis C virus genotype 2a/2b chimeric clones (2011)
  • Author(s): Suda G, Sakamoto N, Itsui Y, Nakagawa M, Tasaka-Fujita M, Funaoka Y, Watanabe T, Nitta S, Kiyohashi K, Azuma S, Kakinuma S, Tsuchiya K, Imamura M, Hiraga N, Chayama K, Watanabe M
  • Organizer: 62th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: San Francisco
  • Year and Date: 2011-11-04
• [Presentation] ITPA gene variant protects against treatment-induced anemia and improves viral clearance by pegylated interferon-alfa and ribavirin therapy in chronic hepatitis C patients (2011)
  • Author(s): Sakamoto N, Azuma S, et al
  • Organizer: 62th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: San Francisco
  • Year and Date: 2011-11-04
• [Presentation] MMP-2 and MMP-14 derived from donor cells enhance therapeutic efficacy of liver cell transplantation in mice (2011)
  • Author(s): Kakinuma S, Sakamoto N, et al
  • Organizer: 62th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: San Francisco
  • Year and Date: 2011-11-04
• [Presentation] A high-content screening assay using infectious fluorescence-tagged hepatitis C virus reveals candidates for small molecule inhibitors of viral entry (2011)
  • Author(s): Kusano-Kitazume A, Sakamoto N, et al
  • Organizer: 62th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: San Francisco
  • Year and Date: 2011-11-04
• [Presentation] Loww of Wnt5A promotes biliary differentiation of murine hepatic stem/progenitor cells (2011)
  • Author(s): Kiyohashi K, Sakamoto N, et al
  • Organizer: 62th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: San Francisco
  • Year and Date: 2011-11-04
• [Presentation] Antiviral effect of a novel interferon-inducible protein, IFI-27, against hepatitis C virus replication (2011)
  • Author(s): Itsuil Y, Sakamoto N, et al
  • Organizer: 62th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: San Francisco
  • Year and Date: 2011-11-04
• [Presentation] Prediction model of ribavirin-induced anemia incorporating ITPA genotype could identify chronic hepatitis C patients at high risk of relapse among virological responders to pegylated-interferon and ribavirin (2011)
  • Author(s): Kurosaki M, Sakamoto N, et al
  • Organizer: 62th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: San Francisco
  • Year and Date: 2011-11-04
• [Presentation] IL28B and ITPA gene variants correlate with treatment efficacy in pegylated-interferon plus ribavirin therapy for chronic hepatitis C (2011)
  • Author(s): Matsuura K, Sakamoto N, et al
  • Organizer: 62th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: San Francisco
  • Year and Date: 2011-11-04
• [Presentation] Mutations in the interferon sensitivity determining region of HCV, age and total ribavirin dose is an independent predictor of relapse among early virological responders to peg-interferon plus ribavirin therapy (2011)
  • Author(s): Kurosaki M, Sakamoto N, et al
  • Organizer: 62th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: San Francisco
  • Year and Date: 2011-11-04
• [Presentation] HCV-NS4B targets STING and abrogates RIG-I-mediated type-I interferon-dependent innate immune response (2011)
  • Author(s): Nitta S, Sakamoto N, Nakagawa M, Kakinuma S, Mishima K, Kusano-KitazumeA, KiyohashiK, Murakawa M, Yoshino K, Tasaka-Fujita M, Watanabe M
  • Place of Presentation: Seattle
  • Year and Date: 2011-09-08
• [Presentation] Development of an RNA polymerase I-driven adenoviral vector and its application in an HCV replication assay (2011)
  • Author(s): Yoshida T, Sakamoto N, et al
  • Organizer: 18th. International Meeting on Hepatitis C Virus & Related Viruses
  • Place of Presentation: Seattle
  • Year and Date: 2011-09-08
• [Presentation] Analysis of interferon signaling by infectious hepatitis C virus clones with substitutions of core amino acids 70 and 91 (2011)
  • Author(s): Sakamoto N, et al
  • Organizer: 18th. International Meeting on Hepatitis C Virus & Related Viruses
  • Place of Presentation: Seattle
  • Year and Date: 2011-09-08
• [Presentation] HCV-NS4B targets STING and abrogates RIG-I-mediated type-I interferon-dependent innate immune response (2011)
  • Author(s): Nitta S, Sakamoto N, Tasaka-Fujita M, et al
  • Organizer: 18th. International Meeting on Hepatitis C Virus & Related Viruses
  • Place of Presentation: Seattle
  • Year and Date: 2011-09-08
• [Presentation] HCV-NS4B蛋白とIFN発現シグナル分子との分子間相互作用の解析 (2011)
  • Author(s): 新田沙由梨、 坂本直哉、藤田めぐみ、北詰晶子、幾世橋佳、船岡祐介、渡辺貴子、植山真由美、小野塚泉、東正新、中川美奈、柿沼晴、渡辺守
  • Organizer: 第48回日本消化器免疫学会
  • Place of Presentation: 金沢
  • Year and Date: 2011-07-21
• [Presentation] HCV-NS4B蛋白とRIG-I惹起性IFN産生シグナル関連蛋白との分子間相互作用の解析 (2011)
  • Author(s): 新田沙由梨、坂本直哉、藤田めぐみ、柿沼晴、村川美也子、北詰晶子、幾世橋佳、船岡祐介、渡辺貴子、植山真由美、小野塚泉、櫻井幸、中川美奈、東正新、渡辺守
  • Organizer: 第47回日本肝臓学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-02
• [Presentation] Development of RNA pol-driven adenovirus vector expressing hepatitis C virus replicon (2011)
  • Author(s): Yamagishi Y, Sakamoto N, et al
  • Organizer: Experimental Biology 2011
  • Place of Presentation: Washington
  • Year and Date: 2011-04-13
• [Presentation] Antiviral effects of interferon-induced proteins GBP-1 and its interactions with hepatitis C virusNS5B protein (2009)
  • Author(s): Itsui Y, Sakamoto N, Nakagawa M, Osajima Y, Tasaka M, Sakurai Y, Suda G, Karakama Y, Mishima K, Yamamoto M, Watanabe T, Ueyama M, Funaoka Y, Azuma S (Chen CH), Kakinuma S, Yauchi T, Watanabe M
  • Organizer: 60th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: Boston
  • Year and Date: 2009-11-02
• [Presentation] インターフェロン発現・作動機構とHCV蛋白 (2009)
  • Author(s): 田坂めぐみ
  • Organizer: 第13回日本肝臓学会大会
  • Place of Presentation: 京都
  • Year and Date: 2009-10-14
• [Presentation] インターフェロン発現・作動機構とHCV蛋白 (2009)
  • Author(s): 藤田めぐみ
  • Organizer: 第13回 日本肝臓学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-10-14
• [Presentation] Establishment and genetic analyses of cytopathogenic HCV-JFH1 mutants by plaque-forming assay (2009)
  • Author(s): Mishima K, Sakamoto N, Sekine-Osajima Y, Nakagawa M, Tasaka M, Nishimura-Sakurai Y, Itsui Y, Wakita T, Watanabe M
  • Place of Presentation: Nice
  • Year and Date: 2009-10-03
• [Presentation] Genotype 2型C型慢性肝炎のインターフェ ロン治療再燃例に対する再治療の試み (2009)
  • Author(s): 渡辺貴子、中川美奈、船岡祐介、植山真由美、三島果子、山本満千、小貫優子、須田剛生、櫻井幸、田坂めぐみ、筬島裕子、井津井康浩、陳正新、坂本直哉、渡辺守
  • Organizer: 第45回日本肝臓学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2009-06-04
• [Presentation] HCV増殖を制御するInterferon誘導蛋白とHCV非構造蛋白の相互作用の解析 (2009)
  • Author(s): 井津井康浩、 坂本直哉、中川美奈、筬島裕子、田坂めぐみ、櫻井幸、須田剛生、三島果子、山本満千、植山真由美、渡辺貴子、 船岡祐介、渡辺守
  • Organizer: 第45回日本肝臓学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2009-06-04
• [Presentation] 細胞障害性HCV-JFH1 subcloneの単離と機能解析 (2009)
  • Author(s): 三島果子、坂本直哉、中川美奈、井津井康浩、筬島裕子、田坂めぐみ、櫻井幸、須田剛生、山本満千、植山真由美、渡辺貴子、船岡祐介、渡辺守
  • Organizer: 第45回日本肝臓学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2009-06-04
• [Presentation] Genotype 2b急性肝炎由来HCVクローンの構築と増殖・粒子形成能の解析 (2009)
  • Author(s): 須田剛生、坂本直哉、中川美奈、田坂めぐみ、櫻井幸、小貫優子、山本満千、渡辺貴子、植山真由美、船岡祐介、井津井康浩、陳正新、今村道雄、茶山一彰、渡辺守
  • Organizer: 第45回日本肝臓学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2009-06-04
• [Presentation] HCVコア変異株培養系を用いたインターフェロン感受性、細胞内増殖、粒子分泌能の解析 (2009)
  • Author(s): 船岡祐介、坂本直哉、井津井康浩、須田剛生、中川美奈、田坂めぐみ、櫻井幸、小貫優子、山本満千、渡辺貴子、植山真由美、陳正新、渡辺守
  • Organizer: 第45回日本肝臓学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2009-06-04
• [Presentation] HCV複製増殖に関連する糖脂質代謝ネットワークの網羅的解析 (2009)
  • Author(s): 櫻井幸、坂本直哉、中川美奈、井津井康浩、田坂めぐみ、小貫優子、須田剛生、三島果子、山本満千、植山真由美、船岡祐介、渡辺貴子、陳正新、渡辺守
  • Organizer: 第45回日本肝臓学会総会
  • Year and Date: 2009-06-04
• [Presentation] HCV genotype 1b 株キメラウイルスを用いたHCV core 領域アミノ酸 70/91 変異株の解析
  • Author(s): 藤田めぐみ、脇田隆宇、加藤孝宣
  • Organizer: 第48回 日本肝臓学会総会
  • Place of Presentation: ホテル日航金沢（石川）
• [Presentation] HCV-NS4B Protein Binds STING and Blocks the RIG-I Mediated IFN Pathway.
  • Author(s): Nitta S, Sakamoto N, Tasaka-Fujita M, Asahina Y, Watanabe M et al
  • Organizer: The 10th JSH Single Topic Conference
  • Place of Presentation: Tokyo
• [Presentation] Association of Gene Expression Involving Innate Immunity and IFN Signaling with Genetic Variation in IL28B in Patients with Chronic Hepatitis C.
  • Author(s): Asahina Y, Murakawa M, Watanabe M et al
  • Organizer: The 10th JSH Single Topic Conference
  • Place of Presentation: Tokyo
  • Invited
• [Presentation] Discovery of N-(morpholine-4-carbonyloxy) amidine compounds as potent inhibitors against hepatitis C virus replication.
  • Author(s): Kusano-Kitazume A, Sakamoto N, Asahina Y, Watanabe M et al
  • Organizer: The 10th JSH Single Topic Conference
  • Place of Presentation: Tokyo
• [Presentation] Impaired induction of IL28B expression in PBMC is associated with null response to interferon therapy in chronic hepatitis C.
  • Author(s): Murakawa M, Sakamoto N, Asahina Y, Watanabe M et al
  • Organizer: 19th International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: Venice
• [Presentation] HCV-NS4B blocks the RIG-I mediated IFN pathway through targeting of STING.
  • Author(s): Nitta S, Sakamoto N, Tasaka-Fujita M, Asahina Y, Watanabe M et al
  • Organizer: 19th International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: Venice
• [Book] 肝癌治療実践のKnack & Pitfalls RFAのoutcome診療ガイドラインに沿った肝癌治療の要点と盲点 (2013)
  • Author(s): 高山忠利、泉並木、朝比奈靖浩
  • Publisher: 文光堂
• [Book] 肝臓病診療 ゴールデンハンドブック 改訂第2版 (2013)
  • Author(s): 泉並木、朝比奈靖浩
  • Publisher: 南江堂
• [Book] 肝炎ウイルスーB型・C型 (2012)
  • Author(s): 熊田博光、朝比奈靖浩
  • Publisher: 医薬ジャーナル社
• [Book] 臨床検査のガイドライン (2012)
  • Author(s): 日本臨床検査医学会ガイドライン作成委員会、朝比奈靖浩
  • Publisher: 株式会社宇宙堂八木書店
• [Patent(Industrial Property Rights)] C型肝炎ウイルスの増殖を抑制する医薬組成物 (2011)
  • Inventor(s): 坂本直哉、渡辺守、北詰晶子、萩原正敏、奥野友紀子
  • Industrial Property Rights Holder: 東京医科歯科大学
  • Industrial Property Number: 2011-194082
  • Filing Date: 2011-09-06