| Project/Area Number |
21590831
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
FUJITA Megumi (TASAKA Megumi) 東京医科歯科大学, 大学院・医歯学総合研究科, 特任助教 (50510369)
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|---|
| Co-Investigator(Kenkyū-buntansha) |
ASAHINA Yasuhiro 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (00422692)
AZUMA Seishin 東京医科歯科大学, 医学部附属病院, 講師 (10376783)
SAKAMOTO Naoya 北海道大学, 医学系研究科, 教授 (10334418)
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| Project Period (FY) |
2009 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | HCV レプリコン / インターフェロン / RIG-I / HCV-NS4B / STING / HCVレプリコン / Cardif / 生体発光共鳴エネルギー転移(Bioliminescence Resonance Energy ransfer) |
|---|
| Research Abstract |
We investigated protein interaction between Hepatitis C virus (HCV)-NS4B and signaling molecules associated with interferon (IFN) production, including Cardif, and STING. The weak interaction between HCV-NS4B and Cardif was observed. Our data, including BiFC analysis, immunoprecipitation assay, and immunostaining, demonstrated that HCV-NS4B specifically bindsSTING. We showed that NS4B suppresses STING-mediated IFN production.
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