Identification of liver disease-specific peptide by sugar chain profiling
| Project/Area Number |
21590839
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Mie University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHIRAKI Katsuya 三重大学, 大学院・医学系研究科, 准教授 (90263003)
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| Co-Investigator(Renkei-kenkyūsha) |
UCHIDA Kazuhiko 筑波大学, 人間総合科学研究科, 准教授 (90211078)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 肝臓学 / 質量分析 / 糖鎖 / 肝細胞癌 / 慢性肝炎 / 非アルコール性脂肪性肝疾患 / 質量分 / 肝細胞がん |
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| Research Abstract |
We identified inter-α-trypsin inhibitor heavy chain 4(ITIH4) peptide as a novel serum biomarker for liver disease using 2D-μHPLC-MALDI-TOF-MS. Also we developed immuno-MS system to deal with many samples. Using this system, we determined the utility of this biomarker peptide for detecting liver cirrhosis and hepatocellular carcinoma(HCC). Moreover, this peptide was useful for detecting non-alcoholic fatty liver diseases. And 35kD protein fragment including this peptide was divided into ten isoforms by the sugar chain modification, and serum isofrom 9 and 10 levels were elevated in early HCC patients. These isoforms are useful biomarker for HCC and may be related to hepatic carcinogenesis.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Prospective study on the outcome of patients with hepatocellular carcinoma registered forliving donor liver transplantation : how long can they wait?2010
Author(s)
Mizuno S, Yokoi H, Shiraki K, Usui M, Sakurai H, Tabata M, Sugimoto K, Takei Y, Yamakado K, Takeda K, Uemoto S, Isaji S
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Journal Title
Transplantation
Volume: 89
Pages: 650-54
Related Report
Peer Reviewed
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[Journal Article] Up-regulation of glypican-3 in human hepatocellular carcinoma2010
Author(s)
Suzuki M, Sugimoto K, Tanaka J, Tameda M, Inagaki Y, Kusagawa S, Nojiri K, Beppu T, Yoneda K, Yamamoto N, Ito M, Yoneda M, Uchida K, Takase K, Shiraki K
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Journal Title
Anticancer Res
Volume: 30
Pages: 5055-61
Related Report
Peer Reviewed
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[Journal Article] Functional cell surface expression of toll-like receptor 9 promotes cell proliferation and survival in human hepatocellular carcinomas2010
Author(s)
Tanaka J, Sugimoto K, Shiraki K, Tameda M, Kusagawa S, Nojiri K, Beppu T, Yoneda K, Yamamoto N, Uchida K, Kojima T, Takei Y
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Journal Title
Int J Oncol
Volume: 37
Pages: 805-14
Related Report
Peer Reviewed
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[Journal Article] Clinical significance of tumor markers in detection of recurrent hepatocellular carcinoma after radiofrequency ablation2010
Author(s)
Beppu T, Sugimoto K, Shiraki K, Tameda M, Kusagawa S, Nojiri K, Tanaka J, Yamamoto N, Takei Y, Takaki H, Uraki J, Nakatsuka A, Yamakado K, Takeda K.
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Journal Title
Int J Mol Med
Volume: 26(3)
Pages: 425-33
NAID
Related Report
Peer Reviewed
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[Journal Article] Classification of hypocholesterolemia lipid patterns using Chol/Trig Combination System2010
Author(s)
Ooi K, Sugimoto K, Shiraki K, Yamamoto N, Tameda M, Beppu T, Tanaka J, Nojiri K, Kusagawa S, Takei Y, Masuda C, Nobori T
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Journal Title
Int J Mol Med
Volume: 25
Pages: 601-6
Related Report
Peer Reviewed
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