| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
We applied tumor specific methylated DNA for prediction of tumor dynamics in HCC cases. The methylated APC and GSTP1 genes were identified as useful marker for discrimination of HCC cells from non-cancerous liver. We established a sensitive assay for quantifying of methylated/non-methylated(Me/NMe) DNA ratio from serum of 100μl. We successfullydetect Me-DNA from all HCC cases examined and no normal control cases showed Me-DNA. The Me/NMe DNA ratio elevated quicker than conventional HCC marker, AFP, when tumor showed recurrence. This novel assay could be a promising approachfor judgmentof theraupic effectof HCCincluding dormant therapy.
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