Analysis of interferon-τsignaling in immune cells of patients with hepatitis C virus infection to establish effective therapeutic strategy.

• Project/Area Number: 21590842
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Osaka University
• Principal Investigator: MIYAGI Takuya 大阪大学, 大学院・医学系研究科, 助教 (80532986)
• Co-Investigator(Kenkyū-buntansha): TAKEHARA Tetsuo 大阪大学, 大学院・医学系研究科, 教授 (70335355)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: C型肝炎 / インターフェロン / シグナル伝達 / 免疫細胞 / NK細胞 / STAT / NK細胞サブセット

Research Abstract

The present study demonstrates altered interferon-αsignaling in natural killer cells in patients with chronic hepatitis C virus infection and suggests the association of this alteration with the persistence of hepatitis C virus infection and the resistance to interferon-α-based therapy.

Research Products

• [Journal Article] Differential alteration of CD56^<bright> and CD56^<dim> natural killer cells in frequency, phenotype, and cytokine response in chronic hepatitis C virus infection (2011)
  • Author(s): MIYAGI, T., SHIMIZU, S., TATSUMI, T., NISHIO, K., HIRAMATSU, N., KANTO, T., HAYASHI, N. & TAKEHARA, T.
  • Journal Title: J Gastroenterol Volume: 46 Pages: 1020-30
  • NAID: 10029563284
  • Peer Reviewed
• [Journal Article] Indications and limitations for aged patients with chronic hepatitis C in pegylated interferon alfa-2b plus ribavirin combination therapy (2011)
  • Author(s): OZE, T., HIRAMATSU, N., YAKUSHIJIN, T., MOCHIZUKI, K., OSHITA, M., HAGIWARA, H., MITA, E., ITO, T., FUKUI, H., INUI, Y., HIJIOKA, T., INADA, M., KAYTAYAMA, K., TAMURA, S., YOSHIHARA, H., INOUE, A., IMAI, Y., KATO, M., MIYAGI, T., YOSHIDA, Y., TATSUMI, T.,
  • Journal Title: J Hepatol Volume: 54 Pages: 604-11
  • Peer Reviewed
• [Journal Article] Differential alteration of CD56^<bright> and CD56^<dim> natural killer cells in frequency, phenotype and cytokine response in chronic hepatitis C virus infection (2011)
  • Author(s): Miyagi T, Tatsumi T, et al
  • Journal Title: Journal of Gastroenterology Volume: 46 Issue: 8 Pages: 1020-1030
  • DOI: 10.1007/s00535-011-0408-8
  • NAID: 10029563284
  • Peer Reviewed
• [Journal Article] Absence of invariant natural killer T cells deteriorates liver inflammation and fibrosis in mice fed high-fat diet (2010)
  • Author(s): MIYAGI, T., TAKEHARA, T., UEMURA, A., NISHIO, K., SHIMIZU, S., KODAMA, T., HIKITA, H., LI, W., SASAKAWA, A., TATSUMI, T., OHKAWA, K., KANTO, T., HIRAMATSU, N. & HAYASHI, N.
  • Journal Title: J Gastroenterol Volume: 45 Pages: 1247-54
  • NAID: 10027700169
  • Peer Reviewed
• [Journal Article] Altered interferon-τ-signaling in natural killer cells from patients with chronic hepatitis C virus infection (2010)
  • Author(s): MIYAGI, T., TAKEHARA, T., NISHIO, K., SHIMIZU, S., KOHGA, K., LI, W., TATSUMI, T., HIRAMATSU, N., KANTO, T. & HAYASHI, N.
  • Journal Title: J Hepatol Volume: 53 Pages: 424-30
  • Peer Reviewed
• [Journal Article] Intracellular staining for analysis of the expression and phosphorylation of signal transducers and activators of transcription(STATs) in NK cells (2010)
  • Author(s): MIYAGI, T., LEE, SH. & BIRON, CA.
  • Journal Title: Methods Mol Biol Volume: 612 Pages: 159-75
  • Peer Reviewed
• [Journal Article] Altered interferon-α-signaling in natural killer cells from patients with chronic hepatitis C virus infection. (2010)
  • Author(s): 宮成琢也
  • Journal Title: Journal of Hepatology Volume: 53 Pages: 424-430
  • Peer Reviewed
• [Journal Article] Intracellular staining for analysis of the expression and phosphorylation of signal transducers and activators of transcription (STATs) in NK cells (2010)
  • Author(s): 宮城琢也
  • Journal Title: Methods in Molecular Biology 612 Pages: 159-175
  • Peer Reviewed
• [Journal Article] C型慢性肝炎における免疫細胞内IFNシグナル伝達機構 (2009)
  • Author(s): 宮城琢也
  • Journal Title: MINOPHAGEN MEDICAL REVIEW 54 Pages: 135-136
• [Presentation] C型慢性肝炎におけるNK細胞サブセットCD56^<bright>/ CD56^<dim>の関与 (2011)
  • Author(s): 宮城琢也
  • Organizer: 第53回日本消化器病学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2011-10-21
• [Presentation] C型慢性肝炎におけるNK細胞サブセットCD56^<bright>/CD56^<dim>の関与 (2011)
  • Author(s): 向井香織
  • Organizer: 第53回日本消化器病学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2011-10-21
• [Presentation] Interferon-α-signaling in natural killer cells is impaired in chronic hepatitis C virus infection (2009)
  • Author(s): 宮城琢也
  • Organizer: 第60回アメリカ肝臓病学会年次集会
  • Place of Presentation: ボストン(米国)
  • Year and Date: 2009-11-02
• [Presentation] Interferon-α-signaling in natural killer cells is impaired in chronic hepatitis C virus infection (2009)
  • Author(s): 宮城琢也
  • Organizer: 第60回アメリカ肝臓病学会年次集会
  • Place of Presentation: ボストン (アメリカ)
  • Year and Date: 2009-11-02
• [Presentation] C型肝炎に対するインターフェロン治療における免疫エフェクター細胞内シグナル伝達解析 (2009)
  • Author(s): 宮城琢也
  • Organizer: 第95回日本消化器病学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2009-05-07
• [Presentation] C型肝炎に対するインターフェロン治療における免疫エフェクター細胞内シグナル伝達解析 (2009)
  • Author(s): 宮城琢也
  • Organizer: 第95回日本消化器病学会総会
  • Place of Presentation: 京王プラザホテル札幌 (北海道)
  • Year and Date: 2009-05-07
• [Book] Annual Review 2011消化器C型肝炎・肝がんにおける自然免疫応答 (2011)
  • Author(s): 宮城琢也
  • Publisher: 中外医学社
• [Book] Annual Review 2011消化器C型肝炎・肝がんにお自然免疫応答 (2011)
  • Author(s): 宮成琢也
  • Publisher: 中外医学社