| Project/Area Number |
21590854
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
OHIRA Hiromasa 福島県立医科大学, 医学部, 教授 (90274951)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOKAWA Junjo 福島県立医科大学, 消化器・リウマチ膠原病内科, 非常勤助教 (00453019)
TAKAHASHI Astushi 福島県立医科大学, 消化器・リウマチ膠原病内科, 助教 (40404868)
ABE Kazumichi 福島県立医科大学, 消化器・リウマチ膠原病内科, 助教 (30468128)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | プロテオソーム / 自己免疫性肝炎 / phosphoenolpyruvate carboxykinase / 自己抗体 / PEPCK / 原発性胆汁性肝硬変 / プロテオーム / 臨床 / 免疫学 |
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| Research Abstract |
In this study, we analyzed autoantibodies in patients with autoimmune hepatitis(AIH) using proteomics tool. In the process of screening of autoantibodies, phosphoenolpyruvate carboxykinase(PEPCK) protein was identified as a target antigen of an autoantibody from serum of patients with AIH. Anti-PEPCK antibody was positive in 33. 3%(10/30) in patients with AIH, 43. 7%(7/16) in patients with primary biliary cirrhosis(PBC)-AIH overlap and 6%(2/30) in patients with PBC, but not detected in healthy control. This antibody is detected by high frequency in AIH patients and its specificity is high. In conclusion, measurement of anti-PEPCK antibody is considered to be useful for diagnosis of autoimmune hepatitis.
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