| Project/Area Number |
21590858
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
|
|---|
| Research Institution | Saitama Medical University |
|---|
Principal Investigator |
|
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| Co-Investigator(Kenkyū-buntansha) |
INAO Mie 埼玉医科大学, 医学部, 講師 (70286037)
SUGAWARA Kayoko 埼玉医科大学, 医学部, 助教 (20406458)
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| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
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| Keywords | オステオポンチン / 転写因子 / 性差 / エストロゲン / C型慢性肝炎 / SNP |
|---|
| Research Abstract |
Osteopontin promoter SNPs(nt-155,-433) were responsible for the development of sexual difference of carcinogenesis in the liver. Promoter assay using male HepG2 cells and female MCF-7 cells revealed that the activities differed between alleles of either of SNPs in both cells. Also, gel-shift assay with nuclear extracts from both cells showed 3 signals ; a common signal for both cells, male-specific SRY and a female-specific indeterminate signal. The intensity of a common signal was different between the allele of nt-155, and that of a female-specific signal was increased following addition of estrogen to MCF-7 cells. These observations suggest that sexual difference of carcinogenesis may develop through these transcriptional factors which play differently between male and female cells.
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