Identification of molecular chaperones as regulators for HCV RNA replication through proteomics approaches
Project/Area Number |
21590867
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Gastroenterology
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Research Institution | National Institute of Infectious Diseases |
Principal Investigator |
AIZAKI Hideki 国立感染症研究所, ウイルス第二部, 室長 (00333360)
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Co-Investigator(Kenkyū-buntansha) |
SUZUKI Teturou 浜松医科大学, 感染症講座, 教授 (00250184)
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | ウイルス / 感染症 / 癌 / 内科 / プロテオーム |
Research Abstract |
To identify the host factors implicated in the regulation of hepatitis C virus(HCV) genome replication, we performed comparative proteome analyses of HCV replication complex(RC)-rich membrane fractions prepared from cells harboring genome-length bicistronic HCV RNA at the exponential and stationary growth phases. We found that CCT plays a role in the replication possibly through an interaction between subunit CCT5 and the viral RNA polymerase NS5B. siRNA-mediated knockdown of CCT5suppressed RNA replication. These suggest that recruitment of the chaperonin by the viral nonstructural proteins to the RC, which potentially facilitate folding of the RC component(s) into the mature active form, may be important for ef! cient replication of the HCV genome.
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Report
(4 results)
Research Products
(92 results)
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[Journal Article] Chaperonin TRiC/CCT participates in replication of hepatitis C virus genome via interaction with the viral NS5B protein2011
Author(s)
Inoue Y, Aizaki H, Hara H, Matsuda M, Ando T, Shimoji T, Murakami K, Masaki T, Shoji I, Homma S, Matsuura Y, Miyamura T, Wakita T, Suzuki T
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Journal Title
Virology
Volume: 410
Pages: 38-47
Related Report
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[Journal Article] Chaperonin TRiC/CCT participates in replication of hepatitis Cvirus genome via interaction with the viral NS5B protein2011
Author(s)
Inoue Y, Aizaki H, Hara H, Matsuda M, Ando T, Shimoji T, Murakami K, Masaki T, Shoji I, Homma S, Matsuura Y, Miyamura T, Wakita T, Suzuki T
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Journal Title
Virology
Volume: 410
Pages: 38-47
Related Report
Peer Reviewed
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[Journal Article] Chaperonin TRiC/CCT participates in replication of hepatitis C virus genome via interaction with the viral NS5B protein.2011
Author(s)
Inoue Y, Aizaki H, Hara H, Matsuda M, Ando T, Shimoji T, Murakami K, Masaki T, Shoji I, Homma S, Matsuura Y, Miyamura T, Wakita T, Suzuki T.
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Journal Title
Virology.
Volume: 410
Pages: 38-47
Related Report
Peer Reviewed
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[Journal Article] Production of infectious hepatitis C virus by using RNA polymerase I-mediated transcription2010
Author(s)
Masaki T, Suzuki R, Saeed M, Mori KI, Matsuda M, Aizaki H, Ishii K, Maki N, Miyamura T, Matsuura Y, Wakita T, Suzuki T
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Journal Title
J Virol
Volume: 84
Pages: 5824-5835
Related Report
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[Journal Article] Production of infectious hepatitis C virus by using RNA polymerase I-mediated transcription.2010
Author(s)
Masaki T, Suzuki R, Saeed M, Mori KI, Matsuda M, Aizaki H, Ishii K, Maki N, Miyamura T, Matsuura Y, Wakita T, Suzuki T.
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Journal Title
J Virol.
Volume: 84
Pages: 5824-5835
Related Report
Peer Reviewed
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[Journal Article] Proteomics analysis of mitochondrial proteins reveals overexpression of a mitochondrial protein chaperone, prohibitin, in cells expressing hepatitis C virus core protein2009
Author(s)
Tsutsumi T, Matsuda M, Aizaki H, Moriya K, Miyoshi H, Fujie H, Shintani Y, Yotsuyanagi H, Miyamura T, Suzuki T, Koike K
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Journal Title
Hepatology
Volume: 50
Pages: 378-386
Related Report
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[Journal Article] Involvement of creatine kinase B in hepatitis C virus genome replication through interaction with the viral NS4A protein2009
Author(s)
Hara H, Aizaki H, Matsuda M, Shinkai-Ouchi F, Inoue Y, Murakami K, Shoji I, Kawakami H, Matsuura Y, Lai MM, Miyamura T, Wakita T, Suzuki T
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Journal Title
J Virol. 83
Pages: 5137-5147
Related Report
Peer Reviewed
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[Journal Article] Proteomics analysis of mitochondrial proteins reveals over expression of a mitochondrial protein chaperone, prohibitin, in cells expressing hepatitis C virus core protein2009
Author(s)
Tsutsumi T, Matsuda M, Aizaki H, Moriya K, Miyoshi H, Fujie H, Shintani Y, Yotsuyanagi H, Miyamura T, Suzuki T, Koike K
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Journal Title
Hepatology 50
Pages: 378-386
Related Report
Peer Reviewed
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[Journal Article] Production of infectious hepatitis C virus by using RNA polymerase I-mediated transcription
Author(s)
Masaki T, Suzuki R, Saeed M, Mori KI, Matsuda M, Aizaki H, Ishii K, Maki N, Miyamura T, Matsuura Y, Wakita T, Suzuki T
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Journal Title
Related Report
Peer Reviewed
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[Presentation] Identification of lipid droplet-associated membrane proteins that are involved in HCV production2011
Author(s)
Aizaki H, Matsumoto Y, Goto K, Watashi K, Suzuki R, Fukasawa M, Hanada K, Sato S, Takahashi N, Matsuura Y, Motojima K, Miyamura T, Suzuki T, Wakita T
Organizer
18^<th> International Meeting on Hepatitis C Virus and Related Viruses
Place of Presentation
米国ワシントン州
Year and Date
2011-09-08
Related Report
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[Presentation] Identification of novel NS5A-associated proteins in the host-cell membrane fraction and their role in HCV life cycle2011
Author(s)
Goto K, Kimura T, Watashi K, Suzuki R, Yamagoe S, Miyamura T, Moriya K, Yotsuyanagi H, Koike K, Suzuki T, Wakita T, Aizaki H
Organizer
18^<th> International Meeting on Hepatitis C Virus and Related Viruses
Place of Presentation
米国ワシントン州
Year and Date
2011-09-08
Related Report
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[Presentation] Identification of lipid droplet-associated membrane proteins that are involved in HCV production2011
Author(s)
Aizaki H, Matsumoto Y, Goto K, Watashi K, Suzuki R, Fukasawa M, Hanada K, Sato S, Takahashi N, Matsuura Y, Motojima K, Miyamura T, Suzuki T, WakitaT
Organizer
18^<th> International Meeting on Hepatitis C Virus and Related Viruses
Place of Presentation
WA, USA
Related Report
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[Presentation] Identification of novel NS5A-associated proteins in the host-cell membrane fraction and their role in HCV life cycle2011
Author(s)
Goto K, Kimura T, Watashi K, Suzuki R, Yamagoe S, Miyamura T, Moriya K, Yotsuyanagi H, Koike K, Suzuki T, Wakita T, Aizaki H
Organizer
18^<th> International Meeting on Hepatitis C Virus and Related Viruses
Place of Presentation
WA, USA
Related Report
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[Presentation] Identification of lipid droplet-associated membrane proteins that are involved in HCV production2009
Author(s)
Aizaki H, Yamamoto M, Goto K, Fukasawa M, Hanada K, Sato S, Takahashi N, Matsuura Y, Miyamura T, Wakita T, Suzuki T
Organizer
16^<th> International Meeting on Hepatitis C Virus and Related Viruses
Place of Presentation
Nice, France
Related Report
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[Presentation] Identification of lipid droplet -associated membrane proteins that are involved in HCV production2009
Author(s)
Aizaki H, Yamamoto M, Goto K, Fukasawa M, Hanada K, Sato S, Takahashi N, Matsuura Y, Miyamura T, Wakita T, Suzuki T
Organizer
16th International Symposium on Hepatitis C Virus and Related Viruses
Place of Presentation
Nice, France
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