| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
Although JFH-1 replicates efficiently and produces infectious virus in cell culture, the relationship between in vitro phenotype and in vivo pathogenesis is still unclear. We synthesized the full-genome JFH-1 constructs with mutations detected in chimpanzees and assessed effects of these mutations on viral production in vitro. Higher virus assembly and lower replication capacity of JFH-1 with these mutations were observed. The control of viral production such as replicati on, assembly and secretion by specific mutations may be a key of viral strategy to evade host defense mechanisms.
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