Development of the new therapy targeted for pancreatic cancer stem cell and cancer cells with EMT status via induction of cellular senescence

• Project/Area Number: 21590870
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所) (2011); Miyagi Cancer Center Research Institute (2010); Tohoku University (2009)
• Principal Investigator: SATOH Kennichi 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん幹細胞研究部, 部長 (10282055)
• Co-Investigator(Kenkyū-buntansha): HAMADA Shin 東北大学, 病院, 医員 (20451560)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 膵癌 / EMT / マイクロRNA / miR-126 / ADAM9

Research Abstract

The aim of this study was to identify the molecules to regulate EMT and the mechanism to control cellular senescence in pancreatic cancer. We revealed that miR 126 was down-regulated in invasive pancreatic cancer compared to non-invasive IPMN. In addition, the expression level of MSX2, inducer of EMT in pancreatic cancer, was found to be utilized for diagnosis of pancreatic cancer. Moreover, MSX2 expressing pancreatic cancer showed the chemoresistance, suggesting that EMT related molecules could be therapeutic target for pancreatic cancer.

Research Products

• [Journal Article] MiR-126 acts as a tumor suppressor in pancreatic cancer cells via the regulation of ADM9 (2012)
  • Author(s): Hamada S, Satoh K, Fujibuchi W, Hirota M, Kanno A, Unno J, Masamune A, Kikuta K, Kume K, Shimosegawa T.
  • Journal Title: Mol Cancer Res Volume: 10 Pages: 3-10
  • Peer Reviewed
• [Journal Article] The homeobox gene MSX2 determines chemosensitivity of pancreatic cancer cells via the regulation of transporter gene ABCG2 (2011)
  • Author(s): Hamada S, Satoh K, Hirota M, Kanno A, Umino J, Ito H, Masamune A, Kikuta K, Kume K, Shimosegawa T.
  • Journal Title: J Cell Physiol Volume: 227 Pages: 729-38
  • Peer Reviewed
• [Journal Article] Evaluation of MSX2 mRNA in brush cytology specimens distinguished pancreatic carcinoma from chronic pancreatitis (2011)
  • Author(s): Satoh K, Hamada S, Kanno A, Ishida K, Ito H, Hirota M, Masamune A, Egawa S, Unno M, Shimosegawa T.
  • Journal Title: Cancer Sci Volume: 102 Pages: 157-61
  • NAID: 10029288885
  • Peer Reviewed
• [Journal Article] The evaluation of MSX2 mRNA expression level in biliary brush cytological specimens (2011)
  • Author(s): Ito H, Satoh K, et al
  • Journal Title: Anticancer Res Volume: 31 Pages: 1011-1017
  • Peer Reviewed
• [Journal Article] The homeobox gene MSX2 determines chemosensitivity of pancreatic cancer cells via the regulation of transporter gene ABCG2 (2011)
  • Author(s): Hamada S, Satoh K, et al
  • Journal Title: J Cell Physiol Volume: 227 Pages: 729-738
  • Peer Reviewed
• [Journal Article] Calcium-binding protein S100P is a novel diagnostic marker of cholangiocarcinoma. (2011)
  • Author(s): Hamada S, Satoh K, et al.
  • Journal Title: Cancer Sci Volume: 102 Pages: 150-156
  • NAID: 10029288859
  • Peer Reviewed
• [Journal Article] Evaluation of MSX2 mRNA in brush cytology specimens distinguished pancreatic carcinoma from chronic pancreatitis. (2011)
  • Author(s): Satoh K, et al.
  • Journal Title: Cancer Sci Volume: 102 Pages: 157-161
  • Peer Reviewed
• [Journal Article] Expression of MSX2 predicts malignancy of branch duct intraductal papillary mucinous neoplasm of the pancreas (2010)
  • Author(s): Satoh K, Hamada S, Kanno A, Hirota M, Umino J, Ito H, Masamune A, Egawa S, Motoi F, Unno F, Shimosegawa T.
  • Journal Title: J Gastoloenterol Volume: 45 Pages: 763-70
  • NAID: 10026707200
  • Peer Reviewed
• [Journal Article] Expression of MSX2 predicts malignancy of branch duct intraductal papillary mucinous neoplasm of the pancreas. (2010)
  • Author(s): Satoh K, et al.
  • Journal Title: J Gastoloenterol Volume: 45 Pages: 763-770
  • Peer Reviewed
• [Journal Article] Expression of MSX2 predicts malignancy of branch duct intraductal papillary mucinous neoplasm of the pancreas. (2010)
  • Author(s): Satoh K, Hamada S, Kanno A, Hirota M, Umino J, Ito H, Masamune A, Egawa S, Unno M, Shimosegawa T.
  • Journal Title: J Gastroenterol. Jan 28(Epub)
  • NAID: 10026707200
  • Peer Reviewed
• [Journal Article] Chronic pancreatitis and pancreatic cancer : prediction and mechanism (2009)
  • Author(s): himosegawa T, Kume K, Satoh K.
  • Journal Title: Clin Gastroenterol Hepatol
  • Peer Reviewed
• [Journal Article] Chronic pancreatitis and pancreatic cancer : prediction and mechanism. (2009)
  • Author(s): Shimosegawa T, Kume K, Satoh K.
  • Journal Title: Clin Gastroenterol Hepatol. 7(11Suppl)
  • Peer Reviewed
• [Presentation] miR126の発現低下は膵癌細胞の浸潤を促進する (2012)
  • Author(s): 佐藤賢一
  • Organizer: 第70回日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-10-05
• [Presentation] miR126の発現低下は膵癌細胞の浸潤を促進する (2011)
  • Author(s): 佐藤賢一、濱田晋、廣田衛久
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-05
• [Presentation] The application of homeobox gene MSX2 down-regulation as a therapeutic strategy for pancreatic cancer (2010)
  • Author(s): Satoh K, Hamada S, Hirota M, Kanno A, Ito H, Shimosegawa T.
  • Organizer: Joint meeting of the international association of pancreatology and the Japan pancreas society2010
  • Place of Presentation: Fukuoka
  • Year and Date: 2010-07-11
• [Presentation] The expression level of MSX2 and S100P in brushing samples during ERCP differentiates pancreatic carcinoma from chronic pancreatitis (2010)
  • Author(s): Satoh K, Hamada S, Hirota M, Kanno A, Ito H, Shimosegawa T.
  • Organizer: DIGESTIVE DISEASE WEEK 2010
  • Place of Presentation: New Orleans, LA
  • Year and Date: 2010-05-03
• [Presentation] 膵胆管擦過細胞におけるS100P発現量解析による膵胆道癌診断への応用 (2010)
  • Author(s): 佐藤賢一
  • Organizer: 第96回日本消化器病学会
  • Place of Presentation: 新潟
  • Year and Date: 2010-04-23
• [Presentation] 膵癌におけるBMPシグナルの役割と臨床応用についての検討 (2009)
  • Author(s): 佐藤賢一、濱田晋、菅野敦, 他
  • Organizer: 第40回 日本膵臓学会 大会
  • Place of Presentation: 東京
  • Year and Date: 2009-07-31