Toll-like receptor expression level on circulating monocyte in cardiomyopathy

• Project/Area Number: 21590905
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Kagoshima University
• Principal Investigator: HAMASAKI Syuichi 鹿児島大学, 医歯学総合研究科, 准教授 (10315434)
• Co-Investigator(Kenkyū-buntansha): TEI Cyuwa 鹿児島大学, 医歯学総合研究科, 教授 (10163891) ; KATAOKA Tetsuro 鹿児島大学, 医学部歯学部附属病院, 助教 (70457662) ; ISHIDA Sanemasa 鹿児島大学, 医学部歯学部附属病院, 講師 (30444889) ; ORIHARA Koji 鹿児島大学, 医歯学総合研究科, 特任助教 (90457664)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 臨床 / 内科 / 循環器 / 心筋症 / 感染症 / 拡張型心筋症 / 心筋炎 / トールライクレセプター2 / 末梢血単球 / CD64 / トールライクレセプター

Research Abstract

We hypothesized that Toll-like receptor 2(TLR2) expression level on monocytes can be useful as a marker of inflammatory activity in inflammatory cardiomyopathy as a new concept which choronic myocarditis evolves to dilated cardiomyopathy. Thus, we tried to validate our hypothesis by comparing pathological findings of species derived from myocardium with TLR2 expression level on monocytes, but our trial ended up in failure. We could not either verify the usefulness of Toll-like receptor 2 expression level on monocytes for establishing the diagnostic criteria of dilated cardiomyopathy as an inflammatory cardiomyopathy. Although the patients who were diagnosed as cardiac sarcoidosis showed extremely higher level of TLR2, which may suggest that TLR2 expression level on monocytes is identified as a significant contributor for cardiac sarcoidosis. Therefore, high TLR2 expression level on monocytes may be an independent risk factor for cardiac sarcosidosis.

Research Products

• [Journal Article] Reevaluation of quantitative flow cytometric analysis for TLR2 on monocytes using F(ab') 2 fragments of monoclonal antibodies (2011)
  • Author(s): Oba R, Orihara K, Kumagai T, Hirai H, Nagata K, Hamasaki S, Tei C, Masataka N. Cytometry A.
  • Volume: 79
  • Pages: 247-255
  • Peer Reviewed
• [Journal Article] Reevaluation of quantitative flow cytometric analysis for TLR2 on monocytes using F(ab')(2) fragments of monoclonal antibodies (2011)
  • Author(s): 大場隆一郎
  • Journal Title: Cytometry A Volume: 79 Issue: 4 Pages: 247-255
  • DOI: 10.1002/cyto.a.21000
  • Peer Reviewed
• [Journal Article] Reevaluation of quantitative flow cytometric analysis for TLR2 on Monocytes using F(ab')(2)fragments of monoclonal antibodies (2011)
  • Author(s): 大場隆一郎
  • Journal Title: Cytometry A Volume: 79 Pages: 247-255
  • Peer Reviewed
• [Journal Article] High expression level of Toll-like receptor 2 on monocytes is an important risk factor for arteriosclerotic disease (2010)
  • Author(s): Kuwahata S, Fujita S, Orihara K, Hamasaki S, Oba R, Hirai H, Nagata K, Ishida S, Kataoka T, Oketani N, Ichiki H, Iriki Y, Saihara K, Okui H, Ninomiya Y, Tei C.
  • Journal Title: Atherosclerosis Volume: 209 Pages: 248-254
  • Peer Reviewed
• [Journal Article] High expression level of Toll-like receptor 2 on monocytes is an important risk factor for atherosclerotic disease (2010)
  • Author(s): 桑波田聡
  • Journal Title: Atherosclerosis Volume: 209 Pages: 248-254
  • Peer Reviewed
• [Journal Article] High expression level of Toll-like receptor 2 on monocytes is an important risk factor for atherosclerotic disease (2010)
  • Author(s): 桑波田聡
  • Journal Title: Atherosclerosis 209 Pages: 248-254
  • Peer Reviewed
• [Journal Article] The role of infection in the development of non-valvular atrial fibrillation : up-regulation of Toll-like receptor 2 expression levels on monocytes (2009)
  • Author(s): Ichiki H, Orihara K, Hamasaki S, Ishida S, Oketani N, Iriki Y, Ninomiya Y, Okui H, Kuwahata S, Fujita S, Matsushita T, Yoshifuku S, Oba R, Hirai H, Nagata K, Tei C.
  • Journal Title: J Cardiol Volume: 53 Pages: 127-135
  • NAID: 10025182813
  • Peer Reviewed
• [Journal Article] The role of infection in the development of non-valvular atrial Fibrillation : Up-regulation of Toll-like receptor 2 expression levels on monocytes (2009)
  • Author(s): 市来仁志
  • Journal Title: J Cardiol 53 Pages: 127-135
  • Peer Reviewed
• [Presentation] Symposium, Modulation of Toll-like receptor 2 on chronic inflammation in patients with atherosclerosis and atrial fibrillation (2012)
  • Author(s): Orihara K, Kuwahata S, Fujita S, Hamasaki S, Ishida S, Kataoka T, Oketani N, Ichiki H, Iriki Y, Okui H, Ninomiya Y, Tei C.
  • Organizer: 第76回日本循環器学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-03-16
• [Presentation] シンポジウム21,循環器疾患の成因における慢性炎症の意義:Modulation of Toll-like receptor 2 on chronic inflammation in patients with atherosclerosis and atrial fibrillation (2012)
  • Author(s): 折原弘治
  • Organizer: 第76回日本循環器学会学術集会
  • Place of Presentation: 福岡(招待講演)
  • Year and Date: 2012-03-16
• [Presentation] Symposium, The up-regulation of Toll-like receptor 2 in patients with atrial fibrillation and atherosclerosis (2011)
  • Author(s): Ichiki H, Orihara K, Hamasaki S, Ishida S, Oketani N, Iriki Y, Okui H, Kuwahata S, Fujita S, Tei C.
  • Organizer: 第15回日本心不全学会
  • Place of Presentation: 鹿児島
  • Year and Date: 2011-10-13
• [Presentation] The up-regulation of Toll-like receptor 2 in patients with atrial fibrillation and atherosclerosis (2011)
  • Author(s): 市来仁志
  • Organizer: 第15回日本心不全学会学術集会
  • Place of Presentation: 鹿児島
  • Year and Date: 2011-10-13
• [Presentation] Featured Research Session ; Angina Pectoris, Key Note Lecture : High-expression level of Toll-like receptor 2 on monocytes as a new risk factor for arteriosclerotic disease (2011)
  • Author(s): 濱崎秀一
  • Organizer: 第75回日本循環器学会総会・学術集会
  • Place of Presentation: 横浜(招待講演)
  • Year and Date: 2011-08-04
• [Presentation] The comparison of biomarkers, left atrial volume, and the extent of CFAE areas in paroxysmal and persistent AF patients who underwent AF ablation solely guided by CFAE (2011)
  • Author(s): Ichiki H, Orihara K, Oketani N, Ishida S, Okui H, Iriki Y, Ninomiya Y, Maenosono R, Matsushita T, Hamasaki S, Oba R, Hirai H, Nagata K, Tei C.
  • Organizer: 第75回日本循環器学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-08-03
• [Presentation] Key note lecture, High expression level of Toll-like receptor 2 on monocytes as a new risk factor for arteriosclerotic disease (2011)
  • Author(s): Hamasaki S, Orihara K, Kuwahata S, Fujita S, Tei C.
  • Organizer: 第75回日本循環器学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-08-03
• [Presentation] Biomarker to predict the AF recurrence after catheter ablation for non-valvular atrial fibrillation (2009)
  • Author(s): Ichiki H, Orihara K, Ishida S, Oketani N, Iriki Y, Ninomiya Y, Okui H, Matsushia T, Fujita S, Kuwahata S, Oba R, Hirai H, Nagata K, Hamasaki S, Tei C.
  • Organizer: 第73回日本循環器学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-03-21
• [Presentation] Role of Infectious Inflammation for Atherogenesis : Toll-like Receptor 2 vs. C-reactive Protein (2009)
  • Author(s): So Kuwahata S, Fujita S, Orihara K, Hamasaki S, Kataoka T, Saihara K, Ishida S, Ichiki H, Oketani N, Ninomiya Y, Okui H, Oba R, Hirai H, Nagata K, Tei C.
  • Organizer: 第73回日本循環器学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-03-21
• [Presentation] Role of infectious inflammation for atherogenesis : Toll-like receptor 2 vs.C-reactive protein (2009)
  • Author(s): 桑波田聡
  • Organizer: 第73回日本循環器学会総会・学術集会
  • Place of Presentation: 大阪国際会議場リーガロイヤルホテル(大阪)
  • Year and Date: 2009-03-20