Imaging of intracellular dynamics of Ca^<2+>-influx-associated molecules and its physiological significance in endothelial cells.
| Project/Area Number |
21590948
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ISSHIKI Masashi 東京大学, 医学部附属病院, 特任准教授 (70302734)
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| Co-Investigator(Renkei-kenkyūsha) |
MIZUNO Risuke 東京大学, 医学部附属病院, 特任助教 (30273080)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | シグナル伝達 / 細胞生物学 / 循環器 / 高血圧 / イメージング / マイクロドメイン / 循環器・高血圧 |
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| Research Abstract |
Intracellular Stim1 localization dynamically changes in response to Ca^<2+> store depletion and its expression level is linked to eNOS activation and NO production via regulation of Ca^<2+> influx level in cultured endothelial cells. We further analyzed endothelium specific Stim1 null mice and confirmed enhanced vascular contraction by phenylephrine, diminished endothelium-dependent vascular relaxation by acetylcholine, and elevation of blood pressure. Thus, Stim1 play a role in blood pressure regulation via eNOS/NO system and endothelial function.
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Report
(4 results)
Research Products
(11 results)
