| Project/Area Number |
21590961
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Hokkaido University |
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Principal Investigator |
BETSUYAKU Tomoko Hokkaido University, 大学院・医学研究科, 准教授 (60333605)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | LCM / マウス / 喫煙 / 細気管支上皮 / miRNA |
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| Research Abstract |
MicroRNAs (miRNAs) are a family of non-coding RNA molecules that post-transcriptionally regulate gene expression. It has recently been discovered that changes in expression of miRNAs are involved in development, carcinogenesis and inflammation. However, it is largely unknown how aging and smoking are associated with differential expression of miRNAs in the lungs. The main problem with analyzing miRNA expression in the lungs is the degree of cellular heterogeneity. The use of tissue homogenates inevitably results in average expression levels of all cells present in the lungs, thus potentially masking any differences in the expression of individual miRNAs within specific cell types. We therefore focused on the changes in miRNA expression in bronchiolar epithelium in vivo, since bronchiolar epithelium is an important site in the pathophysiology of COPD as well as in cigarette smoke (CS)-induced animal models of the disease. We demonstrated changes in miRNAs in mouse bronchiolar epithelium by age and CS exposure. We also found that the cessation of smoking differently affected the miRNA profiling by age.
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