| Project/Area Number |
21590976
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
TAKENAGA Mitsuko 聖マリアンナ医科大学, 医学(系)研究科(研究院), 准教授 (10236490)
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| Co-Investigator(Kenkyū-buntansha) |
OHTA Yuki 聖マリアンナ医科大学, 薬理学, 助教 (60387066)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 閉塞性肺疾患 / 慢性閉塞性肺疾患 / 細胞治療 / 脂肪組織由来体性幹細胞 / 肺気腫 / 再生治療 / 脂肪組織 / 体性幹細胞 / 再生医学 / 脂肪 / 幹細胞 |
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| Research Abstract |
Adipose tissue-derived stem/stromal cells(ASC) transplantation(2.5×10^6/500μl/20min) was performed through intravenous route in porcine pancreatic elastase(PPE)(250 U/kg)-induced emphysema model rats. Pulmonary function(arterial oxygen tension(PaO_2) and alveolar-arterial oxygen tension difference(A-aDO_2) was restored almost to that of normal animals. Enlargement of the alveolar airspaces was inhibited. Hepatocyte growth factor(HGF) and cytokine-induced neutrophil chemoattractant-1(CINC-1) levels were significantly higher in the ASC group even at 2 weeks after transplantation. Sponge implantation with CINC-1 induced neovascular formation with increased HGF. In vitro secretion of HGF and CINC-1 from ASC was promoted in the presence of IL-1β. Some grafted ASC was confirmed to survive at the damaged site. Therefore, not only HGF but also CINC-1 secreted from transplanted and viable ASC, presumably contributed to lung repair through angiogenesis.
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