Adipose tissue-derived stem/stromal cells(ASC) therapy to elastase-elicited pulmonary emphysema in rat model
| Project/Area Number |
21590976
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
TAKENAGA Mitsuko 聖マリアンナ医科大学, 医学(系)研究科(研究院), 准教授 (10236490)
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| Co-Investigator(Kenkyū-buntansha) |
OHTA Yuki 聖マリアンナ医科大学, 薬理学, 助教 (60387066)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 閉塞性肺疾患 / 慢性閉塞性肺疾患 / 細胞治療 / 脂肪組織由来体性幹細胞 / 肺気腫 / 再生治療 / 脂肪組織 / 体性幹細胞 / 再生医学 / 脂肪 / 幹細胞 |
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| Research Abstract |
Adipose tissue-derived stem/stromal cells(ASC) transplantation(2.5×10^6/500μl/20min) was performed through intravenous route in porcine pancreatic elastase(PPE)(250 U/kg)-induced emphysema model rats. Pulmonary function(arterial oxygen tension(PaO_2) and alveolar-arterial oxygen tension difference(A-aDO_2) was restored almost to that of normal animals. Enlargement of the alveolar airspaces was inhibited. Hepatocyte growth factor(HGF) and cytokine-induced neutrophil chemoattractant-1(CINC-1) levels were significantly higher in the ASC group even at 2 weeks after transplantation. Sponge implantation with CINC-1 induced neovascular formation with increased HGF. In vitro secretion of HGF and CINC-1 from ASC was promoted in the presence of IL-1β. Some grafted ASC was confirmed to survive at the damaged site. Therefore, not only HGF but also CINC-1 secreted from transplanted and viable ASC, presumably contributed to lung repair through angiogenesis.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Pulmonary functions, angiogenic growth factors and related ones, and histological changes in elastase-induced pulmonary emphysema rat model2011
Author(s)
Furuya N, Takenaga M, Ohta Y, Tokura Y, Hamaguchi A, Sakamaki A, Kida H, Handa H, Nishine H, Mineshita M, Miyazawa T
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Journal Title
J. St. Marianna Univ
Volume: 2(2)
Pages: 71-77
NAID
Related Report
Peer Reviewed
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[Journal Article] Cell therapy with adipose tissue-derived stem/stromal cells(ASC) for elastase-induced pulmonary emphysema in rats
Author(s)
Furuya N, Takenaga M, Ohta Y, Tokura Y, Hamaguchi A, Sakamaki A, Kida H, Handa H, Nishine H, Mineshita M, Miyazawa M
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Journal Title
Regenerative Med
Volume: (in press)
Related Report
Peer Reviewed
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[Presentation] 脂肪組織を利用した再生治療の試み2009
Author(s)
太田有紀, 井上肇, 古屋直樹, 山野嘉久, 宮沢輝臣, 熊谷憲夫, 武永美津子
Organizer
第18回日本形成外科学会
Place of Presentation
都市センターホテル、東京
Year and Date
2009-10-01
Related Report
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