Establishment of a simple differentiation diagnostic method of glucoprotein tumor markers by the pattern analysis in Western blotting

Research Project

Project/Area Number	21590981
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Respiratory organ internal medicine
Research Institution	University of Tsukuba
Principal Investigator	SATOH Hiroaki 筑波大学, 医学医療系, 教授 (00261800)
Co-Investigator(Kenkyū-buntansha)	OHTSUKA Morio 筑波大学, 医学医療系, 教授 (00143173)
Project Period (FY)	2009 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2011: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000) Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords	癌 / 肺癌 / 腫瘍マーカー / 診断法 / 泳動パターン / 診断 / 糖鎖抗原 / 血清診断
Research Abstract	It is not clear whether structures of glucoprotein tumor markers such carcinoembryonic antigen (CEA) and CA19-9 differ or not in various cancers. Western blot analysis of the expression patterns in various cancers was performed using original antibody against CEA which we made. The expression pattern of them was ladder bands pattern. In serum from lung, bands larger than 150kD were observed, but they were not in serum form other cancer patients and normal control (p=0.0352). Expression patterns were evaluated in such analysis using antibody against CA19-9 in sera from pancreas and colon cancers. A clue of simple differentiation diagnostic method of glucoprotein tumor markers by the pattern analysis in Western blotting was established.