Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Research Abstract |
Cell cycle regulator p27 shuttles between the nucleus and the cytoplasm. Phosphorylation at threonine 198(T198) and 157(T157) increase cell motility. We showed S10 hosphorylation was associated with T198 and T157 phosphorylation in small cell lung cancer(SCLC) cell lines. In 30 surgically resected SCLC specimens, however, immunohistochemistry revealed high expression of phosphorylated p27 at T198 and T157. Leptomycin B or knockdown of cellular CRM1 by siRNA showed additive effects with topoisomerase II inhibitors(Etoposide and Amrubicine) in SCLC cell lines and a fibroblast cell line. There were no correlations between chemosensitivity of amrubicin and mRNA expression levels of the RanBP2, TopoII-alpha and TopoII-beta genes.
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