| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Research Abstract |
Tight junctions are the main intercellular junctions of podocytes of the renal glomerulus under nephrotic conditions. Their requisite components, claudins, still remain to be identified. Absolute quantitative real-time PCR using cDNA of isolated rat glomeruli showed that claudin-5 was the most abundantl claudin expressed in glomeruli. In situ hybridization and immunolocalization studies revealed that claudin-5 was localized mainly in podocytes. Claudin-5 protein was observed on the entire surface of podocytes including slit diaphragms, and was inclined to be concentrated on tight junctions in puromycin aminonucleoside nephrosis. Immunoprecipitation studies using anti-claudin-5 and glomerular lysates revealed the association of claudin-5 and nephrin. It is concluded that claudin-5 is a main claudin expressed in podocytes and that the formation of tight junctions in the nephrosis may be due to local recruitment of claudin-5 rather than due to total upregulation of the claudin protein levels.
|
