Structure and function of tight junctions involved in slit diaphragms
| Project/Area Number |
21591021
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Kidney internal medicine
|
|---|
| Research Institution | Niigata University |
|---|
Principal Investigator |
YAOITA Eishin 新潟大学, 医歯学系, 准教授 (00157950)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Yutaka 新潟大学, 医歯学系, 講師 (40182795)
YAMOTO Tadashi 新潟大学, 医歯学系, 教授 (30092737)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 腎臓 / 足細胞 / 細胞間結合 / 密着結合 / スリット膜 / 糸球体 / claudin / 免疫沈降 / 免疫染色 / リン酸化 / in situ hybridization / RT-PCR |
|---|
| Research Abstract |
Tight junctions are the main intercellular junctions of podocytes of the renal glomerulus under nephrotic conditions. Their requisite components, claudins, still remain to be identified. Absolute quantitative real-time PCR using cDNA of isolated rat glomeruli showed that claudin-5 was the most abundantl claudin expressed in glomeruli. In situ hybridization and immunolocalization studies revealed that claudin-5 was localized mainly in podocytes. Claudin-5 protein was observed on the entire surface of podocytes including slit diaphragms, and was inclined to be concentrated on tight junctions in puromycin aminonucleoside nephrosis. Immunoprecipitation studies using anti-claudin-5 and glomerular lysates revealed the association of claudin-5 and nephrin. It is concluded that claudin-5 is a main claudin expressed in podocytes and that the formation of tight junctions in the nephrosis may be due to local recruitment of claudin-5 rather than due to total upregulation of the claudin protein levels.
|
Report
(4 results)
Research Products
(17 results)
-
-
-
-
[Journal Article] Novel expression of claudin-5 in glomerular podocytes2011
Author(s)
Koda R, Zhao L, Yaoita E, Yoshida Y, Tsukita S, Tamura A, Nameta M, Zhang Y, Fujinaka H, Magdeldin S, Xu B, Narita I, Yamamoto T
-
Journal Title
Cell and Tissue Research
Volume: Vol.343, No.3
Pages: 637-648
Related Report
Peer Reviewed
-
[Journal Article]2011
Author(s)
Sekiguchi S, Suzuki A, Asano S, Nishiwaki-Yasuda K, Shibata M, Nagao S, Yamamoto N, Matsuyama M, Sato Y, Yan K, Yaoita E, Itoh M
-
Journal Title
American Journal of Physiology Renal Physiology
Volume: Vol.300, No.4
Pages: 848-856
Related Report
Peer Reviewed
-
-
-
-
-
[Journal Article] Glomerular proteins related to slit diaphragm and matrix adhesion in the foot processes are highly tyrosine phosphorylated in the normal rat kidney2010
Author(s)
Zhang Y, Yoshida Y, Nameta M, Xu B, Taguchi I, Ikeda T, Fujinaka H, Mohamed SM, Tsukaguchi H, Harita Y, Yaoita E, Yamamoto T
-
Journal Title
Nephrology Dialysis Transplantation
Volume: Vol.25, No.6
Pages: 1785-1795
Related Report
Peer Reviewed
-
-
-
-
[Presentation] 糸球体培養2010
Author(s)
矢尾板永信
Organizer
第53回日本腎臓学会学術総会
Place of Presentation
神戸
Year and Date
2010-06-18
Related Report
-
[Presentation] 糸球体培養2010
Author(s)
矢尾板永信
Organizer
第53回日本腎臓学会
Place of Presentation
神戸国際会議場
Year and Date
2010-06-18
Related Report
-
-
