• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Structure and function of tight junctions involved in slit diaphragms

Research Project

Project/Area Number 21591021
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionNiigata University

Principal Investigator

YAOITA Eishin  新潟大学, 医歯学系, 准教授 (00157950)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Yutaka  新潟大学, 医歯学系, 講師 (40182795)
YAMOTO Tadashi  新潟大学, 医歯学系, 教授 (30092737)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords腎臓 / 足細胞 / 細胞間結合 / 密着結合 / スリット膜 / 糸球体 / claudin / 免疫沈降 / 免疫染色 / リン酸化 / in situ hybridization / RT-PCR
Research Abstract

Tight junctions are the main intercellular junctions of podocytes of the renal glomerulus under nephrotic conditions. Their requisite components, claudins, still remain to be identified. Absolute quantitative real-time PCR using cDNA of isolated rat glomeruli showed that claudin-5 was the most abundantl claudin expressed in glomeruli. In situ hybridization and immunolocalization studies revealed that claudin-5 was localized mainly in podocytes. Claudin-5 protein was observed on the entire surface of podocytes including slit diaphragms, and was inclined to be concentrated on tight junctions in puromycin aminonucleoside nephrosis. Immunoprecipitation studies using anti-claudin-5 and glomerular lysates revealed the association of claudin-5 and nephrin. It is concluded that claudin-5 is a main claudin expressed in podocytes and that the formation of tight junctions in the nephrosis may be due to local recruitment of claudin-5 rather than due to total upregulation of the claudin protein levels.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (17 results)

All 2012 2011 2010

All Journal Article (11 results) (of which Peer Reviewed: 10 results) Presentation (6 results)

  • [Journal Article] ポドサイトにおけるタイト結合2012

    • Author(s)
      矢尾板永信
    • Journal Title

      発達腎研究会誌

      Volume: 19巻(印刷中)

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Comparison of human glomerulus proteomic profiles obtained from low quantities of samples by different mass spectrometry with the comprehensive database2011

    • Author(s)
      Zhang Y, Yoshida Y, Xu B, Magdeldin S, Fujinaka H, Liu Z, Miyamoto M, Yaoita E, Yamamoto T
    • Journal Title

      Proteome Science

      Volume: Vol.9, No.1 Pages: 47-57

    • NAID

      130005454254

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Usage of electrostatic eliminator reduces human keratin contamination significantly in gel-based proteomics analysis2011

    • Author(s)
      Xu B, Zhang Y, Zhao Z, Yoshida Y, Magdeldin S, Fujinaka H, Ismail TA, Yaoita E, Yamamoto T
    • Journal Title

      Journal of Proteomics

      Volume: Vol.74, No.7 Pages: 1022-1029

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel expression of claudin-5 in glomerular podocytes2011

    • Author(s)
      Koda R, Zhao L, Yaoita E, Yoshida Y, Tsukita S, Tamura A, Nameta M, Zhang Y, Fujinaka H, Magdeldin S, Xu B, Narita I, Yamamoto T
    • Journal Title

      Cell and Tissue Research

      Volume: Vol.343, No.3 Pages: 637-648

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article]2011

    • Author(s)
      Sekiguchi S, Suzuki A, Asano S, Nishiwaki-Yasuda K, Shibata M, Nagao S, Yamamoto N, Matsuyama M, Sato Y, Yan K, Yaoita E, Itoh M
    • Journal Title

      American Journal of Physiology Renal Physiology

      Volume: Vol.300, No.4 Pages: 848-856

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of human glomerulus proteomic profiles obtained from low quantities of samples by different mass spectrometry with the comprehe nsive database2011

    • Author(s)
      Ying Zhang
    • Journal Title

      Proteome Science

      Volume: VOL.9 Issue: 1 Pages: 47-57

    • DOI

      10.1186/1477-5956-9-47

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Usage of electrostatic eliminator reduces human keratin contamination significantly in gel-based proteomics analysis2011

    • Author(s)
      許波
    • Journal Title

      Journal of Proteomics

      Volume: VOL.74 Issue: 7 Pages: 1022-1029

    • DOI

      10.1016/j.jprot.2011.03.001

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel expression of claudin-5 in glomerular podocytes.2011

    • Author(s)
      甲田亮
    • Journal Title

      Cell and Tissue Research

      Volume: VOL.343 Pages: 637-648

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphate overload induces podocyte injury via type III Na-dependent phosphate transporter2011

    • Author(s)
      関口佐保子
    • Journal Title

      American Journal of Physiology-Renal physiology

      Volume: VOL.300

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Glomerular proteins related to slit diaphragm and matrix adhesion in the foot processes are highly tyrosine phosphorylated in the normal rat kidney2010

    • Author(s)
      Zhang Y, Yoshida Y, Nameta M, Xu B, Taguchi I, Ikeda T, Fujinaka H, Mohamed SM, Tsukaguchi H, Harita Y, Yaoita E, Yamamoto T
    • Journal Title

      Nephrology Dialysis Transplantation

      Volume: Vol.25, No.6 Pages: 1785-1795

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Glomerular proteins related to slit diaphragm and matrix adhesion in the foot processes are highly tyrosine phosphorylated in the normal rat kidney.2010

    • Author(s)
      Zhang Y
    • Journal Title

      Nephrol Dial Transplant. 25

      Pages: 1785-1795

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 初代培養におけるポドサイトの性質2011

    • Author(s)
      矢尾板永信
    • Organizer
      第54回日本腎臓学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2011-06-11
    • Related Report
      2011 Final Research Report
  • [Presentation] 初代培養におけるポドサィトの性質2011

    • Author(s)
      矢尾板永信
    • Organizer
      日本腎臓学会学術総会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2011-06-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] 糸球体培養2010

    • Author(s)
      矢尾板永信
    • Organizer
      第53回日本腎臓学会学術総会
    • Place of Presentation
      神戸
    • Year and Date
      2010-06-18
    • Related Report
      2011 Final Research Report
  • [Presentation] 糸球体培養2010

    • Author(s)
      矢尾板永信
    • Organizer
      第53回日本腎臓学会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2010-06-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Adherens junction proteins in glomerular podocytes of quail kidney2010

    • Author(s)
      矢尾板永信
    • Organizer
      2010 Experimental Biology
    • Place of Presentation
      Anahein, CA, USA
    • Year and Date
      2010-04-26
    • Related Report
      2011 Final Research Report
  • [Presentation] Adherens junction proteins in glomerular podocytes of quail kidney.2010

    • Author(s)
      矢尾板永信
    • Organizer
      2010 Experimental Biology
    • Place of Presentation
      Anahein, CA, USA.s
    • Year and Date
      2010-04-26
    • Related Report
      2010 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi