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Role of glycosylation-related enzymes in cultured podocytes using honeycomb-patterned polymer films

Research Project

Project/Area Number 21591022
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionDokkyo Medical University (2010-2011)
Niigata University (2009)

Principal Investigator

TAKEDA Tetsuro  獨協医科大学, 医学部, 教授 (10361924)

Co-Investigator(Kenkyū-buntansha) SAITO Akihiko  新潟大学, 医歯学総合研究科, 特任教授 (80293207)
Co-Investigator(Renkei-kenkyūsha) TANAKA Masaru  山形大学, 理工学研究科, 教授 (00322850)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords糸球体上皮細胞 / ハニカム膜 / 慢性腎臓病 / 糖転移酵素
Research Abstract

Glomerular epithelial cells(podocytes) play a key role in the prevention of proteinuria, are important determinants of end-stage kidney disease. Surface charge alterations of the podocyte may induce proteinuric injury. Cultured podocytes are currently being used to uncover unique functions of podocytes that can then be validated in vivo. Therefore, we examined a culture of podocytes on self-organized honeycomb-patterned films(honeycomb films). As a result, podocyte maintained their function for the mRNA expression of unique genes. In addition, we investigated the expression of glycosylation-related enzymes in cultured podocytes. We found that a honeycomb film showed higher podocyte function and angiotensin II stimulation decreased the mRNA level of these enzymes. These results suggest that glycosylation-related enzymes contribute to maintain the negative surface charge of podocytes.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (24 results)

All 2012 2011 2010 2009

All Journal Article (13 results) (of which Peer Reviewed: 7 results) Presentation (9 results) Book (2 results)

  • [Journal Article] Significance of Urinary Full-Length and Ectodomain Forms of Megalin in Patients With Type 2 Diabetes2012

    • Author(s)
      Ogasawara S, et al.
    • Journal Title

      Diabetes Care

      Volume: (印刷中) Issue: 5 Pages: 1112-1118

    • DOI

      10.2337/dc11-1684

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 糸球体病理所見の足細胞のfoot process effacementとは(Q & A)2011

    • Author(s)
      竹田徹朗
    • Journal Title

      日本医事新報

      Volume: 4538 Pages: 52-54

    • Related Report
      2011 Final Research Report
  • [Journal Article] 糸球体病理所見の足細胞のfoot process effacementとは2011

    • Author(s)
      竹田徹朗
    • Journal Title

      日本医事新報

      Volume: 4538 Pages: 52-54

    • Related Report
      2011 Annual Research Report
  • [Journal Article] 腎機能障害患者への一般的治療対応法2011

    • Author(s)
      堀賢一郎、竹田徹朗
    • Journal Title

      成人病と生活習慣病

      Volume: 41 Pages: 408-411

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Molecular mechanisms of receptor-mediated endocytosis in the renal proximal tubular epithelium2010

    • Author(s)
      Saito A, Sato H, Iino N, and Takeda T
    • Journal Title

      Journal of Biomed and Biotechnol

      Volume: 403272 Pages: 1-7

    • DOI

      10.1155/2010/403272

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms of receptor-mediated endocytosis in the renal proximal tubular epithelium2010

    • Author(s)
      Saito A, Sato H, Iino N, Takeda T
    • Journal Title

      J Biomed Biotechnol

      Volume: Volume2010

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 診断と治療のTopics慢性腎臓病(CKD)の診断とHIV感染症診療における留意点2010

    • Author(s)
      田邊嘉也、竹田徹朗、成田一衛
    • Journal Title

      HIV感染症とAIDSの治療

      Volume: 1巻 Pages: 30-37

    • NAID

      40018726391

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Regulation of megalin expression in cultured proximal tubule cells by angiotensin II type 1A receptor-and insulin-mediated signaling cross-talk2009

    • Author(s)
      Hosojima M, Sato H, Yamamoto K, Kaseda R, Soma T, Kobayashi A, Suzuki A, Kabasawa H, Takeyama A, Ikuyama K, Iino N, Nishiyama A, Thekkumkara TJ, Takeda T, Suzuki Y, Gejyo F, and Saito A
    • Journal Title

      Endocrinology

      Volume: 150 Issue: 2 Pages: 871-878

    • DOI

      10.1210/en.2008-0886

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Megalin and nonmuscle myosin heavy chain IIA interact with the adaptor protein Disabled-2 in proximal tubule cells2009

    • Author(s)
      Hosaka K, Takeda T, Iino N, Hosojima M, Sato H, Kaseda R, Yamamoto K, Kobayashi A, Gejyo F, and Saito A
    • Journal Title

      Kidney Int

      Volume: 75 Issue: 12 Pages: 1308-1315

    • DOI

      10.1038/ki.2009.85

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 尿中podocyte(ポドサイト),尿中ポドカリキシン2009

    • Author(s)
      竹田徹朗
    • Journal Title

      Medical Technology

      Volume: 37(6) Pages: 564-566

    • Related Report
      2011 Final Research Report
  • [Journal Article] Megalin and nonmuscle myosin heavy chain IIA interact with the adaptor protein Disabled-2 in proximal tubule cells.2009

    • Author(s)
      Hosaka K, Takeda T, …Saito A
    • Journal Title

      Kidney Int 75

      Pages: 1308-1315

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of megalin expression in cultured proximal tubule cells by angiotensin II type 1A receptor- and insulin-mediated signaling cross-talk2009

    • Author(s)
      Hosojima M, …, Takeda T, …, Satito A
    • Journal Title

      Endocrinology 150

      Pages: 871-878

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 糸球体障害マーカー 尿中podocyte、尿中ポドカリキシン2009

    • Author(s)
      竹田徹郎
    • Journal Title

      Medical Technology 37

      Pages: 564-566

    • Related Report
      2009 Annual Research Report
  • [Presentation] 培養近位尿細管上皮細胞におけるLPS-TNFα-ERK1/2シグナル経路を介したメガリンの発現調節機構2011

    • Author(s)
      佐藤博慶、竹田徹朗, ら
    • Organizer
      第54回日本腎臓学会学術集会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2011-06-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] CKDの病態把握と予後推定における尿中メガリン測定の意義2011

    • Author(s)
      小笠原真也、竹田徹朗, ら
    • Organizer
      第54回日本腎臓学会学術集会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2011-06-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] 生活習慣改善プログラムによる尿中アルブミン排泄量の減少効果2011

    • Author(s)
      山本佳子、細島康宏, 蒲澤秀門, 飯野則昭, 竹田徹朗, 鈴木芳樹, 成田一衛, 荒川正昭, 斎藤亮彦
    • Organizer
      第54回日本腎臓学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2011 Final Research Report
  • [Presentation] Low calorie diet with formula food reduces albuminuria in proportion to the improvement in fractional excretion of uric acid in obese men2010

    • Author(s)
      T Takeda, K Yamamoto, N Iino, I Narita, A Saito
    • Organizer
      ISN-Nexus Symposium 2010 : The Kidney and the Vascular System
    • Place of Presentation
      Kyoto
    • Related Report
      2011 Final Research Report
  • [Presentation] Low calorie diet with formula food reduces albuminuria in proportion to the improvement in fractional excretion of uric acid in obese men2010

    • Author(s)
      T Takeda, K Yamamoto, N Iino, I Narita, A Saito
    • Organizer
      ISN-Nexus Symposium 2010 : The Kidney and The Vascular System
    • Place of Presentation
      Kyoto
    • Related Report
      2010 Annual Research Report
  • [Presentation] Low calorie diet reduces albuminuria in proportion to the improvement in serum leptin level and fractional excretion of uric acid in obese men2009

    • Author(s)
      Tetsuro Takeda, Keiko Yamamoto, Noriaki Iino, Ichiei Narita, Akihiko Saito
    • Organizer
      American Society of Nephrology Renal Week 2009
    • Place of Presentation
      San Diego, California, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] 肥満者に対するフォーミュラ食を用いた減量プログラムによるアルブミン尿の減少効果2009

    • Author(s)
      竹田徹朗, 山本佳子, 飯野則昭, 斎藤亮彦, 鈴木芳樹, 下条文武
    • Organizer
      第106回日本内科学会総会・講演会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] Effects of lifestyle modification with combined aerobic and resistance exercise on urinary albumin excretion in the population with metabolic syndrome2009

    • Author(s)
      Keiko Yamamoto, Michihiro Hosojima, Mariko Saito, Junta Tanaka, Masao Hayashi, Noriaki Iino, Tetsuro Takeda, Yoshiki Suzuki, Fumitake Gejyo, Masaaki Arakawa, Akihiko Saito
    • Organizer
      3rd International Congress on Prediabetes and the Metabolic Syndrome
    • Place of Presentation
      Nice, France
    • Related Report
      2011 Final Research Report
  • [Presentation] Low calorie diet reduces albuminuria in proportion to the improvement in serum leptin level and fractional excretion of uric acid in obese men2009

    • Author(s)
      Takeda T, Yamamoto K, Iino N, Narita I, Saito A
    • Organizer
      The 42^<nd> Annual Meeting of the American Society of Nephrology
    • Place of Presentation
      San Diego, California, USA
    • Related Report
      2009 Annual Research Report
  • [Book] 腎臓症候群上2012

    • Author(s)
      川本進也、竹田徹朗
    • Publisher
      日本臨床社
    • Related Report
      2011 Final Research Report
  • [Book] 腎臓症候群:中毒性腎障害2012

    • Author(s)
      川本進也
    • Total Pages
      8
    • Publisher
      日本臨床社
    • Related Report
      2011 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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