Transplantation of allogenic fetal membrane-derived mesenchymal stem cells protect kidney

• Project/Area Number: 21591028
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Osaka University
• Principal Investigator: ISAKA Yoshitaka 大阪大学, 医学系・研究科, 准教授 (00379166)
• Co-Investigator(Kenkyū-buntansha): TAKAHARA Shiro 大阪大学, 医学系研究科, 寄附講座教授 (70179547) ; YAMAHARA Kenichi 国立循環器病センター, 再生医療部, 室長 (50450888)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 幹細胞 / 移植 / 腎炎 / 虚血再灌流傷害 / 再生 / 間葉系幹細胞移植 / 再生医療 / 卵膜由来幹細胞 / 尿細管細胞 / 間葉系線細胞移植 / サイトカイン / 虚血再還流傷害

Research Abstract

Mesenchymal stem cell(MSC) is an attractive therapeutic cell source for treating renal diseases. MSC administration has been shown to improve renal function, although underlying mechanisms are incompletely understood. We recently showed that allogenic fetal membrane-derived MSC(FM-MSC), which is available non-invasively in large amounts, had renoprotective effect in an experimental glomerulonephritis model. Here, we investigated whether allogenic FM-MSC administration could protect kidneys from anti-Thy-1 glomerulonephritis or ischemia/ reperfusion(I/ R) injury. Histological examination revealed that FM-MSC administration significantly suppressed glomerular injury or I/ R injury. Administered FM-MSC were rarely observed in kidneys, but administration of FM-MSC increased serum IL-10 levels. In addition, renoprotective effects of FM-MSC were abolished by using anti-IL-10 neutralization antibody, suggesting that IL-10 would be one of the candidate factors to protect rat kidney from kidney injury. We concluded that allogenic FM-MSC transplantation is a potent therapeutic strategy for the treatment of kidney diseases.

Research Products

• [Journal Article] Allogenic fetal membrane-derived mesenchymal stem cells contribute to renal repair in experimental glomerulonephritis (2010)
  • Author(s): Tsuda H, Yamahara K, Ishikane S, Otani K, Nakamura A, Sawai K, Ichimaru N, Sada M, Taguchi A, Hododa H, Tsuji M, Kawachi H, Horio M, Isaka Y, Kangawa K, Takahara S, Ikeda T.
  • Journal Title: Am J Physiol Renal Physiol Volume: 299 Pages: 1004-1013
  • Peer Reviewed
• [Journal Article] Allogenic fetal membrane-derived mesenchymal stem cells contribute to renal repair in experimental glomerulonephritis. (2010)
  • Author(s): Tsuda H, Yamahara K, Ishikane S.Otani K, Nakamura A, Sawai K, Ichimaru N, Sada M, Taguchi A, Hosoda H, Tsuji M, Kawachi H, Horio M, Isaka Y, Kangawa K, Takahara S, Ikeda T.
  • Journal Title: Am J Physiol Renal Physiol. Volume: 299
  • Peer Reviewed