Protective role of FSP1-positive podocytes in the glomerular injury

• Project/Area Number: 21591036
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: University of Fukui (2011); Nara Medical University (2009-2010)
• Principal Investigator: IWANO Masayuki 福井大学, 医学部, 教授 (20275324)
• Co-Investigator(Kenkyū-buntansha): KUBO Atsushi 奈良県立医科大学, 医学部, 助教 (30316062)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: ポドサイト / FSP1 / 糸球体保護 / アポトーシス / バイオマーカー / FSPI

Research Abstract

The number of FSP1-positive podocytes was elevated in active glomerular damage, especially in crescentic glomerulonephritis(GN). Patients with crescentic GN exhibited elevated levels of urinary FSP1, suggesting the potential use of urinary FSP1 to screen for active and ongoing glomerular damage, such as the formation of cellular crescents. To investigate the role of FSP1-positive podocytes, we generated various kinds of experimental nephritis using FSP1 transgenic mice and FSP1 knockout mice. However, we cannot confirm the protective role of FSP1-positive podocytes in these models.

Research Products

• [Journal Article] Decreased renalα-Klotho expression in early diabetic nephropathy in humans and mice and its possible role in urinary calcium excretion (2012)
  • Author(s): Asai O, Nakatani K, Tanaka T, Sakan H, Imura A, Yoshimoto S, Samejima K, Yamaguchi Y, Matsui M, Akai Y, Konishi N, Iwano M, Nabeshima Y, Saito Y
  • Journal Title: Kidney Int Volume: 81(6) Issue: 6 Pages: 539-47
  • DOI: 10.1038/ki.2011.423
  • Peer Reviewed
• [Journal Article] Clinical significance of fibroblast-specific protein-1 expression on podocytes in patients with focal segmental glomerulosclerosis (2012)
  • Author(s): Samejima K, Nakatani K, Suzuki D, Asai O, Sakan H, Yoshimoto S, Yamaguchi Y, Matsui M, Akai Y, Toyoda M, Iwano M, Saito Y
  • Journal Title: Nephron Clin Pract Volume: 120(1) Issue: 1 Pages: c1-c7
  • DOI: 10.1159/000334184
  • Peer Reviewed
• [Journal Article] Urinary FSP1 is a biomarker of crescentic GN (2012)
  • Author(s): Iwano M, Yamaguchi Y, Iwamoto T, Nakatani K, Matsui M, Kubo A, Akai Y, Mori T, Saito Y
  • Journal Title: J Am Soc Nephrol Volume: 23(2) Issue: 2 Pages: 209-14
  • DOI: 10.1681/asn.2011030229
  • Peer Reviewed
• [Journal Article] Pdx1 and Ngn3 overexpression enhances pancreatic differentiation of mouse ES cell-derived endoderm population (2011)
  • Author(s): Kubo A, Stull R, Takeuchi M, Bonham K, Gouon-Evans V, Sho M, Iwano M, Saito Y, Keller G, Snodgrass R
  • Journal Title: PLoS One Volume: 6(9) Issue: 9 Pages: e24058-e24058
  • DOI: 10.1371/journal.pone.0024058
  • Peer Reviewed
• [Journal Article] Resolution of mesangial light chain deposits three years after high-dose melphalan with autologous peripheral blood stem cell transplantation. (2011)
  • Author(s): Harada Y, Iwano M, et al
  • Journal Title: Clin Nephrol Volume: 74 Pages: 384-388
  • Peer Reviewed
• [Journal Article] Fractalkine expression and CD16+monocyte accumulation in glomerular lesions : association with their severity and diversity in lupus models (2010)
  • Author(s): Nakatani K, Yoshimoto S, Iwano M, Asai O, Samejima K, Sakan H, Terada M, Hasegawa H, Nose M, Saito Y
  • Journal Title: Am J Physiol Renal Physiol Volume: 299(1) Issue: 1 Pages: F207-F216
  • DOI: 10.1152/ajprenal.00482.2009
  • Peer Reviewed
• [Journal Article] The homeobox gene Hex regulates hepatocyte differentiation from embryonic stem cell-derived endoderm (2010)
  • Author(s): Kubo A, Kim YH, Irion S, Kasuda S, Takeuchi M, Ohashi K, Iwano M, Dohi Y, Saito Y, Snodgrass R, Keller G
  • Journal Title: Hepatology Volume: 51(2) Issue: 2 Pages: 633-41
  • DOI: 10.1002/hep.23293
  • Peer Reviewed
• [Journal Article] EMT and TGF-beta in renal fibrosis (2010)
  • Author(s): Iwano M
  • Journal Title: Front Biosci(Schol Ed) Volume: 2 Pages: 229-38
  • Peer Reviewed
• [Journal Article] Fractalkine expression and CD16+ monocytes accumulation in glomerular lesions : Association with their severity and diversity in lupus models. (2010)
  • Author(s): Nakatani K, Iwano M, et al
  • Journal Title: Am J Physiol Renal Physiol Volume: 299
  • Peer Reviewed
• [Journal Article] EMT and TGF-beta in renal fibrosis (2010)
  • Author(s): Iwano M
  • Journal Title: Front Biosci 2 Pages: 229-238
  • Peer Reviewed
• [Journal Article] Reduction of circulating soluble fms-like tyrosine kinase-1 plays a significant role in renal dysfunction-associated aggravation of atherosclerosis (2009)
  • Author(s): Onoue K, Uemura S, Takeda Y, Somekawa S, Iwama H, Imagawa K, Nishida T, Morikawa Y, Takemoto Y, Asai O, Soeda T, Okayama S, Ishigami K, Nakatani K, Kawata H, Horii M, Nakajima T, Akai Y, Iwano M, Saito Y
  • Journal Title: Circulation Volume: 120(24) Issue: 24 Pages: 2470-7
  • DOI: 10.1161/circulationaha.109.867929
  • Peer Reviewed
• [Journal Article] Epithelial-mesenchymal transition as a potential explanation for podocyte depletion in diabetic nephropathy (2009)
  • Author(s): Yamaguchi Y, Iwano M, Suzuki D, Nakatani K, Kimura K, Harada K, Kubo A, Akai Y, Toyoda M, Kanauchi M, Neilson EG, Saito Y
  • Journal Title: Am J Kidney Dis Volume: 54(4) Issue: 4 Pages: 653-64
  • DOI: 10.1053/j.ajkd.2009.05.009
  • Peer Reviewed
• [Journal Article] Epithelial-mesenchymal transition as a potential explanation for podocyte depletion in diabetic nephropathy (2009)
  • Author(s): Yamaguchi Y, Iwano M, et al.
  • Journal Title: Am J Kidney Dis 54 Pages: 653-664
  • Peer Reviewed
• [Presentation] 半月体病変判定における尿中FSP1の臨床的意義 (2011)
  • Author(s): 岩野正之, 他
  • Organizer: 第54回日本腎臓学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2011-06-15
• [Presentation] 新しいバイオマーカーとしての尿中FSP1の臨床的意義 (2010)
  • Author(s): 山口通雅、岩野正之, ら
  • Organizer: 日本腎臓学会学術総会
  • Place of Presentation: 兵庫
  • Year and Date: 2010-06-16
• [Presentation] 尿中FSP1測定法の確立と臨床的意義 (2009)
  • Author(s): 岩野正之, 他
  • Organizer: 第52回日本腎臓学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-06-05
• [Presentation] 尿中FSP1測定法の確立と臨床的意義 (2009)
  • Author(s): 岩野正之, ら
  • Organizer: 日本腎臓学会学術総会
  • Place of Presentation: 神奈川
  • Year and Date: 2009-06-05
• [Remarks]
  • URL: http://www.naramed-u.ac.jp/