Identification of quantitative trait loci for diabetic nephropathy in KK-Ay/Ta mice

• Project/Area Number: 21591039
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Juntendo University
• Principal Investigator: TOMINO Yasuhiko 順天堂大学, 医学部, 教授 (60130077)
• Co-Investigator(Kenkyū-buntansha): GODA Tomohito 順天堂大学, 医学部, 助教 (20365604) ; TSNIMOTO Mituo 順天堂大学, 医学部, 助教 (00420836) ; HAGIWARA Sinji 順天堂大学, 医学部, 助教 (70445568)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 糖尿病腎症 / KK-Ay/Taマウス / QTL / ACR / HbA1c / KK-A^y/Taマウス / QTL解析 / mapping / 尿中アルブミン / KK-Ay / Taマウス

Research Abstract

We studied candidate gene loci for diabetic nephropathy using quantitative trait locus(QTL) analysis of KK-Ay/Ta×BALB/cA F2 intercross mice.
We examined 270(KK-Ay/Ta×BALB/cA) F2 intercross mice. Genotypes were investigated using microsatellite markers with QTL analysis.
ACR in mice at 20 weeks and ACR gain showed a suggestive linkage to chromosome 9. Gene loci contributing to HbA1c indicated a significant linkage to chromosome 7 at 8 and 12 weeks(designed Fbw-1). QTL analysis of KK-Ay/Ta mice revealed several new loci contributing to diabetic nephropathy and related phenotypes. Thus, it appears that type 2 diabetes and nephropathy of KK-Ay/Ta mice have different genetic factors.

Research Products

• [Journal Article] (2011)
  • Author(s): 青木竜弥、富野康日己
  • Journal Title: Journal of Nephrology Volume: 25巻 Pages: 127-136
  • Peer Reviewed
• [Journal Article] Identification of quantitative trait loci for diabetic nephropathy in KK-Ay/Ta mice (2011)
  • Author(s): Tatsuya Aoki
  • Journal Title: Journal of nephrology Volume: 25 Pages: 127-136
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): 青木竜弥、富野康日己
  • Journal Title: 順天堂医学 Volume: 56巻 Pages: 107-115
  • Peer Reviewed
• [Journal Article] 2型糖尿病腎症モデルマウス(KK-A^y/Taマウス)におけるQTL解析による腎症疾患感受性遺伝子座の検討 (2010)
  • Author(s): 青木竜弥、谷本光生、萩原晋二、村越真紀、石川祐史、古川雅子、合田朋仁、堀越哲、富野康日己
  • Journal Title: 順天堂医学 Volume: 56 Pages: 107-108
  • NAID: 10029498125
  • Peer Reviewed
• [Journal Article] 2型糖尿病腎症モデルマウス(KK-A^y/Taマウス)におけるQTL解析による腎症疾患感受性遺伝子座の検討 (2010)
  • Author(s): 青木竜弥, 谷本光生, 萩原晋二, 村越真紀, 石川祐史, 古川雅子, 合田朋仁, 堀越哲, 富野康日己
  • Journal Title: 順天堂医学 56(in press)
  • NAID: 10029498125
  • Peer Reviewed
• [Presentation] Identification of quantitative trait loci for diabetic nephropathy in KK-Ay/Ta mice (2010)
  • Author(s): 青木竜弥、富野康日己
  • Organizer: 第12回アジア太平洋腎臓学会議
  • Place of Presentation: ソウル(韓国)
  • Year and Date: 2010-06-05
• [Presentation] 2型糖尿病腎症モデルマウスを用いた腎症疾患感受性遺伝子座の検討 (2010)
  • Author(s): 青木竜弥、金子滋、谷本光生、萩原晋二、村越真紀、石川祐史、豊田一恵、合田朋仁、船曳和彦、堀越哲、富野康日己
  • Organizer: アジア太平洋腎臓学会議
  • Place of Presentation: ソウル、韓国
  • Year and Date: 2010-06-05
• [Presentation] Identification of quantitative trait loci for diabetic nephropathy in KK-Ay/Ta mice (2009)
  • Author(s): 青木竜弥、富野康日己
  • Organizer: 第42回米国腎臓学会議
  • Place of Presentation: サンディエゴ(米国)
  • Year and Date: 2009-10-27
• [Presentation] 2型糖尿病腎症モデルマウスを用いた腎症疾患感受性遺伝子座の検討 (2009)
  • Author(s): 青木竜弥、富野康日己
  • Organizer: 第53回日本腎臓学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-06-09
• [Presentation] 2型糖尿病腎症モデルマウスを用いた腎症疾患感受性遺伝子座の検討 (2009)
  • Author(s): 青木竜弥, 金子滋, 谷本光生, 萩原晋二, 村越真紀, 石川祐史, 豊田一恵, 合田朋仁, 船曳和彦, 堀越哲, 富野康日己
  • Organizer: 日本腎臓学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-06-04