Kidney regeneration using xeno-metanephros as a scaffold
| Project/Area Number |
21591042
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
YOKOO Takashi 東京慈恵会医科大学, 医学部, 講師 (70301538)
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| Co-Investigator(Renkei-kenkyūsha) |
OONISHI Akira 独立行政法人農業生物紫衣減研究所, 遺伝子組換え家畜センター, 研究副主幹 (30414890)
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| Project Period (FY) |
2009 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 腎臓再生 / 間葉系幹細胞 / 発生工学 / 異種移植 |
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| Research Abstract |
We established xenotransplantation models that control endogenous mesenchymal stem cell (MSC) differentiation into mature erythropoietin (EPO)-producing tissue in a niche provided by a developing xenometanephros. Transplantation of rat metanephroi into mouse omentum, and similarly pig metanephroi into cat omentum, led to the recruitment of host cells and EPO production. EPO-expressing cells were not differentiated from integrating vessels because they did not co-express endothelial markers (Tie-2 and VE-cadherin). Instead, EPO-expressing cells were shown to be derived from circulating host cells, as shown by EGFP expression in the grown transplants of chimeric mice bearing bone marrow from a transgenic mouse expressing EGFP under the control of the EPO promoter. These results suggest that donor cell recruitment and differentiation in a xenotransplanted developing organ may be consistent between species. Using metanephroi from transgenic ER/E2F1 suicide-inducible mice, the xeno-tissue component could be eliminated, leaving autologous EPO-producing tissue.
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Report
(5 results)
Research Products
(69 results)
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[Journal Article] Xenotransplanted embryonic kidney provides a niche for endogenous mesenchymal stem cell differentiation into erythropoietin-producing tissue2012
Author(s)
Matsumoto K, Yokoo T,Matsunari H, Iwai S, Yokote S, Teratani T, Gheisari Y, Tsuji O, Okano H, Utsunomiya Y, Hosoya T, Okano HJ, Nagashima H, Kobayashi E
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Journal Title
Stem Cells
Volume: 30
Pages: 1228-1235
Related Report
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[Journal Article] Relationship between renal function and the risk of recurrent atrial fibrillation following catheter ablation2011
Author(s)
Tokuda M, Yamane T, Matsuo S, Ito K, Marui R, Hioki M, Tanigawa S, Nakane T, Yamashita S, Inada K, Shibayama K, Miyanaga S, Yoshida H, Miyazaki H, Date T, Yokoo T, Yoshimura M
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Journal Title
Heart
Volume: 97(2)
Pages: 137-142
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