Involvement of L-type calcium channels in high extracellular calcium-induced increase in cytoplasmic calcium concentration in parathyroid cells isolated from the patients with secondary hyperparathyroidism.
Project/Area Number |
21591063
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Kidney internal medicine
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Research Institution | Jikei University School of Medicine |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
OHKIDO Ichiro 東京慈恵会医科大学, 医学部, 助教 (40372966)
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 二次性副甲状腺機能亢進症 / Ca^<2+>チャネル / 副甲状腺ホルモン / Klotho / モデルマウス / 遺伝子多型 / 心筋L型Ca^<2+>チャネル |
Research Abstract |
Patch-clamp and fluorescence measurements of cytoplasmic Ca2+concentration([Ca2+] i) were performed to directly detect extracellular Ca2+entry into cultured parathyroid cells from patients with secondary hyperparathyroidism. Cells loaded with fluo-3 AM or fluo-4 AM showed a transient increase in fluorescence(Ca2+transient) following 10 s exposure to 150 mM K+solution in the presence of mM external Ca2+. The Ca2+transient was completely inactivated after 30-40 s exposure to the high K+solution, was reduced by dihydropyridine antagonists, and enhanced by FPL-64176, an L-type Ca2+-channel agonist.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Dihydropyridine-and voltage-sensitive calcium entry in human parathyroid cells2009
Author(s)
Yokoyoma K, Matsuba D, Adachi-Akahane S, Takeyama H, Tabei I, Suzuki A, Shibasaki T, Iida R, Ohkido I, Hosoya T, Suda N
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Journal Title
Experimental Physiology
Volume: 94(7)
Pages: 847-855
Related Report
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