| Project/Area Number |
21591101
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Neurology
|
|---|
| Research Institution | Kawasaki Medical School |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OHSAWA Yutaka 川崎医科大学, 医学部, 講師 (80246511)
MURAKAMI Tatsufumi 川崎医科大学, 医学部, 准教授 (30330591)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 神経分子病態学1 / 筋ジストロフィー / 再生医療 / シグナル伝達 / 発生・分化 / 神経科学 |
|---|
| Research Abstract |
Processing of β-dystroglycan(β-DG) by matrix metalloproteinases(MMPs) has been considered to play a fundamental role in muscle degeneration in sarcoglycan(SG)-deficient muscular dystrophy. Here we generate triple knockout(TKO) mice deficient to MMP-2, MMP-9, and γ-SG to investigate in vivo processing of β-DG by MMP-2, MMP-9.Muscle pathology of TKO mice was comparable to that of γ-SG-knockout mice. Unexpectedly, the 30-kDa-processed form of β-DG was present in TKO mouse muscles likewise γ-SG-null mouse muscles. These findings indicate that MMPs other than MMP-2/9 participate in the molecular mechanism underlying the processing of β-DG in sarcoglycan-deficient muscular dystrophy.
|
