An analysis of endothelial activation under cerebral ischemia using liposomes, which targets E-selectin.

• Project/Area Number: 21591106
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurology
• Research Institution: Fujita Health University (2011); National Center for Geriatrics and Gerontology (2009-2010)
• Principal Investigator: WAKITA Hideaki 藤田保健衛生大学, 医学部・内科, 教授 (80416172)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 神経分子病態学 / 脳血管障害 / 炎症 / 神経科学 / 脳神経疾患 / 発現制御

Research Abstract

A novel liposome conjugated to Sialyl Lewis X(SLX-liposome), which targets E-selectin has been developed. In this study, we investigated the capacity of SLX-liposome containing anti-inflammatory agent for neuroprotection in cerebral ischemia using a rat model of transient middle cerebral artery occlusion(TMCAO). SLX-liposomes blocked E-selectin on luminal endothelium and attenuated infarct volume after TMCAO. These results suggest that SLX-liposomes have the potential for the treatment targeted to endothelial cells in acute ischemic stroke.

Research Products

• [Journal Article] Dental pulp derived CD31-/CD146-side population stem/progenitor cells enhance recovery of focal cerebral ischemia in rats (2011)
  • Author(s): Sugiyama M, Iohara K, Wakita H, Hattori H, Ueda M, Matsushita K, Nakashima M
  • Journal Title: Tissue Engineering Part A Volume: 17(9-10) Issue: 9-10 Pages: 1303-1311
  • DOI: 10.1089/ten.tea.2010.0306
  • Peer Reviewed
• [Journal Article] Watershed Infarcts in a multiple microembolic model of monkey (2011)
  • Author(s): Maki T, Wakita H, Mase M, Itagaki I, Saito N, Ono F, Adachi K, Ito H, Takahashi R, Ihara M, Tomimoto H
  • Journal Title: Neuroscience Letters Volume: 499 Issue: 2 Pages: 80-83
  • DOI: 10.1016/j.neulet.2011.05.036
  • NAID: 120003238944
  • Peer Reviewed
• [Journal Article] E-selectin targeting to visualize tumors in vivo (2010)
  • Author(s): Hirai M, Hiramatsu Y, Iwashita S, Otani T, Chen L, Li YG, Okada M, Oie K, Igarashi K, Wakita H, Seno M
  • Journal Title: Contrast Media & Molecular Imaging Volume: 5(2) Issue: 2 Pages: 70-77
  • DOI: 10.1002/cmmi.367
  • Peer Reviewed
• [Journal Article] miRNAによるE-selectinの抑制 (2010)
  • Author(s): 吉崎嘉一、脇田英明、大隅典子、高橋慶吉
  • Journal Title: 炎症と免疫 Volume: 18(2) Pages: 130-134
• [Journal Article] E-selectin targeting to visualize tumors in vivo. (2010)
  • Author(s): Hirai M, Hiramatsu Y, Iwashita S, Otani T, Chen L, Li Y G, Okada M, Oie K, Igarashi K, Wakita H, Seno M
  • Journal Title: Contrast Media & Molecular Imaging Volume: VOL.5 Pages: 70-77
  • Peer Reviewed
• [Presentation] E-selectinを標的としたリポソームによる細胞接着分子阻止とグリア細胞活性化の抑制 (2011)
  • Author(s): 脇田英明、足立香代、丸山和佳子
  • Organizer: 第52回日本神経学会学術大会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-05-19
• [Presentation] 血管性認知症の実験モデル(シンポジウム1,血管性認知症のニューホライズン) (2010)
  • Author(s): 脇田英明
  • Organizer: 第29回日本認知症学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-11-05
• [Presentation] E-selectinを標的としたリポソームによる細胞接着分子阻止とグリア細胞活性化の抑制 (2010)
  • Author(s): 脇田英明、足立香代
  • Organizer: 第51回日本神経学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-05-22
• [Presentation] セレクチンを標的としたリポソームによる細胞接着分子抑制効果の検討 (2010)
  • Author(s): 脇田英明、足立香代
  • Organizer: 第35回日本脳卒中学会総会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-04-16
• [Presentation] マウス血管性認知症モデルに対するSialyl Lewis X結合リポソームの有効性の検討 (2010)
  • Author(s): 脇田英明、足立香代、武田和也、國本正子、渡邉淳、丸山和佳子
  • Organizer: 第29回日本認知症学会学術集会
  • Place of Presentation: 名古屋
• [Presentation] マウス血管性認知症モデル白質病変での細胞接着分子発現とグリア細胞活性化の検討 (2009)
  • Author(s): 脇田英明、吉崎嘉一
  • Organizer: 第50回日本神経学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2009-05-22