Oxidative stress and phospholipid oxidation : mechanism for the facilitation of monocyte/macrophage adhesion

• Project/Area Number: 21591165
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Nippon Medical School
• Principal Investigator: OIKAWA Shinichi 日本医科大学, 医学部, 教授 (30142946)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: リン脂質 / 酸化 / 単球 / 接着 / 脂質代謝異常 / 酸化変性 / PCOOH / ICAM-1 / Rac / マクロファージ / Rac1

Research Abstract

In this study, we investigated the mechanism underlying the induction of THP-1 monocytic cell adhesion to ICAM-1 by phosphatidylcholine hydroperoxide(PCOOH), a primary oxidation product of phosphatidylcholine. Our results indicate that PCOOH may induce the cell adhesion via Rac activation, actin polymerization, and localization of LFA-1.We also found that non-oxidized phosphatidylserine induces THP-1 cell adhesion to ICAM-1 as well as PCOOH.

Research Products

• [Journal Article] Involvement of Rac GTPase activation in phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to ICAM-1 (2011)
  • Author(s): Asai A. Okajima F., Nakajima Y., Nagao M., Nakagawa K., Miyazawa T., Oikawa S.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 406 Issue: 2 Pages: 273-277
  • DOI: 10.1016/j.bbrc.2011.02.032
  • Peer Reviewed
• [Journal Article] 動脈硬化性疾患におけるリン脂質フォスファチジルコリンの酸化変性 (2011)
  • Author(s): 浅井明、及川眞一
  • Journal Title: オレオサイエンス Volume: 11 Pages: 419-424
  • NAID: 130004650681
• [Presentation] フォスファチジルセリンはTHP-1細胞のICAM-1への接着を亢進する (2011)
  • Author(s): 浅井明、仲川清隆、宮澤陽夫、及川眞一
  • Organizer: 日本過酸化脂質・抗酸化物質学会第19回年会
  • Place of Presentation: 仙台
  • Year and Date: 2011-12-17
• [Presentation] Biochemistry and measurement of phosphatidylcholine hydroperoxide(PCOOH): implications for atherosclerosis(シンポジウム) (2011)
  • Author(s): 浅井明、仲川清隆、永島和幸、宮澤陽夫、及川眞一
  • Organizer: 第43回日本動脈硬化学会総会・学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2011-07-16
• [Presentation] Biochemistry and measurement of phosphatidylcholine hydroperoxide (PCOOH) : implications for atherosclerosis (2011)
  • Author(s): 浅井明、仲川清隆、永島和幸、宮澤陽夫、及川眞一
  • Organizer: 第43回日本動脈硬化学会総会・学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2011-07-16
• [Presentation] Phosphatidylcholine hydroperoxide-induced Rac activation : its involvement in THP-1 cell adhesion to ICAM-1 via actin polymerization (2011)
  • Author(s): A. Asai, K. Nakagawa, F. Okajima, Y. Nakajima, M. Nagao, T. Miyazawa, S. Oikawa
  • Organizer: 79th European Atherosclerosis Society Congress
  • Place of Presentation: Gothenburg, Sweden
  • Year and Date: 2011-06-28
• [Presentation] Phosphatidylcholine hydroperoxide-induced Rac activation : its involvement in THP-1 cell adhesion to ICAM-1 via actin polymerization (2011)
  • Author(s): Asai A, Nakagawa K, Okajima F, Nakajima Y, Nagao M, Miyazawa T, Oikawa S
  • Organizer: 79th European Atherosclerosis Society Congress
  • Place of Presentation: ヨーテボリ(スウェーデン)
  • Year and Date: 2011-06-28
• [Presentation] Phosphatidylcholine hydroperoxide(PCOOH)によるRac GTPasesの活性化: THP-1細胞におけるアクチン骨格形成と接着誘導への関与 (2010)
  • Author(s): 浅井明、岡島史宜、仲川清隆、官澤陽夫、及川眞一
  • Organizer: 第18回生体パーオキサイド研究会
  • Place of Presentation: 仙台
  • Year and Date: 2010-08-21
• [Presentation] Phosphatidylcholine hydroperoxide(PCOOH)によるRac GTPasesの活性化:THP-1細胞におけるアクチン骨格形成と接着誘導への関与 (2010)
  • Author(s): 浅井明、岡島史宜、仲川清隆、官澤陽夫、及川眞一
  • Organizer: 第18回生体パーオキサイド研究会
  • Place of Presentation: ホテル仙台プラザ(仙台市)
  • Year and Date: 2010-08-21
• [Presentation] Lipid hydroperoxide and atherosclerosis (2009)
  • Author(s): S. Oikawa
  • Organizer: 1st International Conference on Lipid Hydroperoxide Biology and Medicine
  • Place of Presentation: Sendai. Japan
  • Year and Date: 2009-11-05
• [Presentation] Lipid hydroperoxide and atherosclerosis (2009)
  • Author(s): Shinichi Oikawa
  • Organizer: 1^<st> International Conference on Lipid Hydroperoxide Biology and Medicine Sendai 2009
  • Place of Presentation: 仙台市
  • Year and Date: 2009-11-05
• [Presentation] Involvement of Rho family proteins on phosphatidylcholine hydroperoxide-induced polymerization and cell adhesion in THP-1 cells (2009)
  • Author(s): A. Asai, F. Okajima, K. Tanimura, Y. Nakajima, M. Nagao, M. Sudo, T. Harada, K. Nakagawa, T. Miyazawa, S. Oikawa
  • Organizer: 8th International Congress of Coronary Artery Disease
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 2009-10-13
• [Presentation] INVOLVEMENT OF RHO FAMILY PROTEINS ON PHOSPHATIDYLCHOLINE HYDROPEROXIDE-INDUCED ACTIN POLYMERIZATION AND CELL ADHESION IN THP-1 CELLS (2009)
  • Author(s): Akira Asai, Fumitaka Okajima, Kyoko Tanimura, Yasushi Nakajima, Mototsugu Nagao, Mariko Sudo, Taro Harada, Kiyotaka Nakagawa, Teruo Miyazawa, Shinichi Oikawa
  • Organizer: 8^<th> International Congress of Coronary Artery Disease
  • Place of Presentation: プラハ (チェコ共和国)
  • Year and Date: 2009-10-13
• [Presentation] 酸化ストレスと動脈硬化(シンポジウム) (2009)
  • Author(s): 及川眞一
  • Organizer: 第63回日本栄養・食糧学会大会
  • Place of Presentation: 長崎
  • Year and Date: 2009-05-21
• [Presentation] 酸化ストレスと動脈硬化 (2009)
  • Author(s): 及川眞一
  • Organizer: 第63回日本栄養・食糧学会大会
  • Place of Presentation: 長崎市
  • Year and Date: 2009-05-21
• [Presentation] Involvement of Rac1 activation on actin polymerization and cell adhesion to intracellular adhesion molecule-1 in phsphatidylcholine hydroperoxide-treated THP-1 cells (2009)
  • Author(s): A. Asai, K. Nakagawa, F. Okajima, K. Tanimura, Y. Nakajima, M. Nagao, M. Sudo, T. Harada, T. Miyazawa, S. Oikawa
  • Organizer: 1st International Conference on Lipid Hydroperoxide Biology and Medicine
  • Place of Presentation: Sendai. Japan
• [Presentation] Involvement of Rac1 activation on actin polymerization and cell adhesion to intracellular adhesion molecule-1 in phsphatidylcholine hydroperoxide-treated THP-1 cells (2009)
  • Author(s): Akira Asai, Kiyotaka Nakagawa, Fumitaka Okajima, Kyoko Tanimura, Yasushi Nakajima, Mototsugu Nagao, Mariko Sudo, Taro Harada, Teruo Miyazawa, Shinichi Oikawa
  • Organizer: 1^<st> International Conference on Lipid Hydroperoxide Biology and Medicine Sendai 2009
  • Place of Presentation: 仙台市