Molecular mechanism of autophagy in follicular lymphoma cells

• Project/Area Number: 21591196
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: University of Fukui
• Principal Investigator: YOSHIDA Akira 福井大学, 医学部・附属病院, 講師 (80252005)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: Bcl-2 / 濾胞性リンパ腫 / オートファジー / LC-II / ABT-737 / Beclin-1

Research Abstract

We investigated the cell death mechanism of Bcl-2 over expressing human leukemia and lymphoma cells. We found that etoposide treatment increased Beclin-1 expression, the conversion of the soluble form of microtubule-associated protein 1 light chain 3(LC3) to the autophagic vesicle-associated form LC3-II and the occurence of lysosomes/autophagosomes in Bcl-2 over expressing cells. The autophagy inhibitor chloroquine enhanced the cytotoxicity of etoposide in Bcl-2 over expressing lymphoma cells. These data indicate that autophagy may play an important role as protective response after etoposide treatment.

Research Products

• [Journal Article] Marked upregulation of Survivin and Aurora-B kinase are associated with disease progression in the myelodysplastic syndromes (2012)
  • Author(s): Yoshida A, Zokumasu K, Wano Y, Yamauchi T, Imamura S, Takagi K, Kishi S, Urasaki Y, Tohyama K, Ueda T
  • Journal Title: Haematologica Volume: 97 Issue: 9 Pages: 1372-1379
  • DOI: 10.3324/haematol.2011.055681
  • Peer Reviewed
• [Journal Article] Camptothecin induces DNA strand breaks and is cytotoxic in stimulated normal lymphocytes. (2011)
  • Author(s): Yamauchi, T., Yoshida, A., Ueda T
  • Journal Title: Oncol Rep Volume: 25 Pages: 347-352
  • Peer Reviewed
• [Journal Article] Camptothecin induces DNA strand breaks and is cytotoxic in stimulated normal lymphocytes (2011)
  • Author(s): Yamauchi T, Yoshida A, Ueda T
  • Journal Title: Oncol Rep Volume: 25(2) Issue: 2 Pages: 347-52
  • DOI: 10.3892/or.2010.1100
  • Peer Reviewed
• [Journal Article] Glutathione S-transferase M1 inhibits dexamethasone-induced apoptosis in association with the suppression of Bim through dual mechanisms in a lymphoblastic leukemia cell line (2010)
  • Author(s): Hosono, N., Kishi, S., Iho, S., Urasaki, Y., Yoshida, A., Kurooka, H., Yokota, Y., Ueda, T
  • Journal Title: Cancer Sci. Volume: 101(3) Pages: 767-773
  • NAID: 10026589943
  • Peer Reviewed
• [Journal Article] Glutathione S-transferase M1 inhibits dexamethasone-induced apoptosis in association with the suppression of Bim through dual mechanisms in a lymphoblastic leukemia cell line. (2010)
  • Author(s): Hosono N, Kishi S, Iho S, Urasaki Y, Yoshida A, Kurooka H, Yokota Y, Ueda T.
  • Journal Title: Cancer Science Volume: 101 Pages: 767-73
  • NAID: 10026589943
  • Peer Reviewed
• [Journal Article] Intracellular cytarabine triphosphate production correlates to deoxycytidine kinase/cytosolic 5'-nucleotidase II expression ratio in primary acute myeloid leukemia cells (2009)
  • Author(s): Yamauchi T, Negoro E, Kishi S, Takagi K, Yoshida A, Urasaki Y, Iwasaki H, Ueda T
  • Journal Title: Biochem Pharmacol Volume: 77(12) Pages: 1780-6
  • Peer Reviewed
• [Presentation] The indolocarbazole derivative Go6976 suppresses the growth of leukemia cells with FLT3 mutations (2012)
  • Author(s): Yoshida, A, Zokumasu, k., Ueda T
  • Organizer: American Association for Cancer Research Annual meeting 2012
  • Place of Presentation: Chicago Illinois, USA
  • Year and Date: 2012-04-02
• [Presentation] Marked Upregulations of Survivin and Aurora-B Kinase Are Associated with Disease Progression in the Myelodysplastic Syndromes (2011)
  • Author(s): Yoshida, A. Zokumasu K., Yamauchi, T., Takagi, K., Kishi, S., Urasaki, Y., Tohyama, K., Ueda T.
  • Organizer: 53rd American Society of Hematology Annual meeting
  • Place of Presentation: San Diego CA, USA
  • Year and Date: 2011-12-12
• [Presentation] Yoshida, A.Zokumasu K., Yamauchi, T., Takagi, K., Kishi, S., Urasaki, Y., Tohyama, K., Ueda T. : Marked Upregulations of Survivin and Aurora-B Kinase Are Associated with Disease Progression in the Myelodysplastic Syndromes (2011)
  • Author(s): Yoshida A, Ueda T
  • Organizer: 53rd American Society of Hematology Annual meeting
  • Place of Presentation: San Diego CA, USA
  • Year and Date: 2011-12-05
• [Presentation] The indolocarbazole, Go6976, suppresses the growth of leukemia cells with FLT3 mutations (2011)
  • Author(s): Yoshida A, Zokumasu K, Ueda T
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-04
• [Presentation] Marked upregulation of Survivin and Aurora-B kinase are associated with disease progression in the Myelodysplastic Syndromes (2011)
  • Author(s): Yoshida, A
  • Organizer: The 2nd Japanese Society of Hematology International Symposium 2011
  • Place of Presentation: 長崎
  • Year and Date: 2011-04-23
• [Presentation] Potent cytotoxic activity of Go6976 in FLT3-driven leukemic cells. (2010)
  • Author(s): Yoshida A, Ueda T.
  • Organizer: 第72回日本血液学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2010-09-25
• [Presentation] Potent cytotoxic activity of Go6976 in FLT3-driven leukemic cells (2010)
  • Author(s): Yoshida A, Ueda T
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2010-09-10
• [Presentation] ハイリスクMDSではSurvivinの著明な高発現が検出される (2009)
  • Author(s): 吉田明,田中沙季,山内高弘,高木和貴,岸愼治,浦崎芳正,通山薫,上田孝典
  • Organizer: 第71回日本血液学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2009-10-12
• [Book] 2.造血器腫瘍の治療.総論A化学療法剤. (2011)
  • Author(s): 吉田明,上田孝典
  • Publisher: 中外医学社
• [Book] 白血病・リンパ腫・骨髄腫-今日の診断と治療.第4版 (2011)
  • Author(s): 吉田明, 上田孝典
  • Publisher: 中外医薬社
• [Book] 分子標的治療5.ボルテゾミブ. (2009)
  • Author(s): 吉田明,上田孝典
  • Publisher: 中外医学社