Establishment of epigenetic pattern during cell differentiation

• Project/Area Number: 21591204
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: 財団法人東京都医学総合研究所 (2011); Tokyo Metropolitan Organization for Medical Research (2009-2010)
• Principal Investigator: KITAJIMA Kenji 財団法人東京都医学総合研究所, 生体分子先端研究分野, 主席研究員 (10346132)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 血液内科学 / ES細胞 / 血液細胞 / 転写因子 / 細胞分化 / エピジェネティクス

Research Abstract

Using mouse embryonic stem cell differentiation induction system, we investigated the role of GATA-1, required for terminal erythroid differentiation, on establishments of erythroid-specific epigenetic pattern. We found that GATA-1 would establish the erythroid-specific epigenetic pattern during cell fate determination from multipotent hematopoietic progenitor cells into erythroid lienage.

Research Products

• [Journal Article] iPS細胞から造血幹細胞の誘導 (2011)
  • Author(s): 北島健二,原孝彦
  • Journal Title: 医学のあゆみ Volume: 239 Pages: 1397-401
• [Journal Article] Multiple ETS Family Proteins Regulate PF4 Gene Expression by Binding to the Same ETS Binding Site (2011)
  • Author(s): Okada Y, Nobori H, Shimizu M, Watanabe M, Yonekura M, Nakai T, Kamikawa Y, Wakimura A, Funahashi N, Naruse H, Watanabe A, Yamasaki D, Fukada S, Yasui K, Matsumoto K, Sato T, Kitajima K, Nakano T, Aird WC, Doi T
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] In vitro generation of HSC-like cells from murine ESCs/iPSCs by enforced expression of LIM-homeobox transcription factor Lhx2 (2011)
  • Author(s): Kitajima K, Minehata K, Sakimura K, Nakano T, Hara T
  • Journal Title: Blood Volume: 117 Pages: 3748-58
  • Peer Reviewed
• [Journal Article] Multiple ETS Family Proteins Regulate PF4 Gene Expression by Binding to the Same ETS Binding Site (2011)
  • Author(s): Okada Y
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] In vitro generation of HSC-like cells from murine ESCs/iPSCs by enforced expression of LIM-homeobox transcription factor Lhx2 (2011)
  • Author(s): Kitajima K
  • Journal Title: Blood Volume: 117 Pages: 3748-3758
  • Peer Reviewed
• [Journal Article] iPS細胞から造血幹細胞の誘導 (2011)
  • Author(s): 北島健二
  • Journal Title: 医学のあゆみ Volume: 239 Pages: 1397-1401
• [Journal Article] Disabled-2 is required for mesoderm differentiation of murine embryonic stem cells (2010)
  • Author(s): Huang C, Cheng J, Kitajima K, Nakano T, Yeh C, Chong K, Tseng C
  • Journal Title: J Cell Physiol Volume: 225 Pages: 92-105
  • Peer Reviewed
• [Journal Article] Novel role for the intraflagellar transport protein CMG-1 in regulating the transcription of cyclin-D2, E-cadherin and integrin-alpha family genes in mouse spermatocyte-derived cells (2010)
  • Author(s): Ohbayashi K, Tanaka K, Kitajima K, Tamura K, Hara T
  • Journal Title: Genes Cells Volume: 15 Pages: 699-710
  • Peer Reviewed
• [Journal Article] Disabled-2 is required for mesoderm differentiation of murine embryonic stem cells (2010)
  • Author(s): Huang C
  • Journal Title: J.Cell.Physiol. Volume: 225 Pages: 92-105
  • Peer Reviewed
• [Journal Article] Calyculin A retraction of mature megakaryocytes proplatelets from embryonic stem cells (2009)
  • Author(s): Tamaru S, Kitajima K, Nakano T, Eto K, Yazaki A, Kobayashi T, Matsumoto T, Wada H, Katayama N, Nishikawa M
  • Journal Title: Biochem Biophys Res Commun Volume: 366 Pages: 763-8
  • Peer Reviewed
• [Journal Article] Increase of hematopoietic progenitor and suppression of endothelial gene expression by Runx1 expression during in vitro ES differentiation (2009)
  • Author(s): Sakai E, Kitajima K, Sato A, Nakano T
  • Journal Title: Exp Hematol Volume: 37 Pages: 334-45
  • Peer Reviewed
• [Journal Article] Calyculin A retraction of mature megakaryocytes proplatelets from embryonic stem cells (2009)
  • Author(s): Tamaru S
  • Journal Title: Biochem Biophys Res Commun 366 Pages: 763-768
  • Peer Reviewed
• [Journal Article] Increase of hematopoietic progenitor and suppression of endothelial gene expression by Runx1 expression during in vitro ES differentiation (2009)
  • Author(s): Sakai E
  • Journal Title: Exp Hematol 37 Pages: 334-345
  • Peer Reviewed
• [Presentation] Ectopic expression of Lhx2 in muscle satellite cells inhibits their myotube differentiation (2011)
  • Author(s): Kodaka Y, Kitajima K, Matsuda R, Hara T
  • Organizer: The 34th Annual Meeting of the Molecular biology Society of Japan
  • Place of Presentation: Yokohama
• [Presentation] Effects of ectopic expression of LIM-homeobox transcription factor lhx2 on in vitro differentiation of ESCs/iPSCs (2011)
  • Author(s): Kitajima K, Hara T
  • Organizer: 44th Annual Meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: Ginowan
• [Presentation] In vivo analyses of mouse iPSC-derived HSC-like cells generated by enforced expression of LIM-homeobox transcription factor Lhx2 (2011)
  • Author(s): Kitajima K, Hara T
  • Organizer: The 9th Stem Cell Research Symposium
  • Place of Presentation: Tokyo
• [Presentation] Induction and expansion of pluripotent stem cells-derived engraftable hematopoietic progenitor cells by LIM-homeodomain transcription factor lhx2 (2010)
  • Author(s): Kitajima K, Hara T
  • Organizer: 5th International Symposium on GATA factors
  • Place of Presentation: Sendai
• [Presentation] Long-term repopulation of adult hematopoiesis by in vitro differentiated blood cells from ESCs/iPSCs by LIM homeobox transcription factor, Lhx2 (2010)
  • Author(s): Kitajima K, Zheng J, Minehata K, Nakano T, Hara T
  • Organizer: The 8th Stem Cell Research Symposium
  • Place of Presentation: Awaji
• [Presentation] The role of Foxo1 in the development of hematopoietic cells (2010)
  • Author(s): Minehata K, Kitajima K, Hara T
  • Organizer: DECODE Winter Workshop 2010
  • Place of Presentation: Yuzawa
• [Presentation] Induction of in vivo hematopoiesis-repopulating cells from mouse pluripotent stem cells by LIM-homeobox transcription factor, lhx2 (2010)
  • Author(s): Kitajima K, Zheng J, Minehata K, Nakano T, Hara T
  • Organizer: DECODE Winter Workshop 2010
  • Place of Presentation: Yuzawa
• [Presentation] Induction and expansion of pluripotent stem cells-derived engraftable hematopoietic progenitor cells by LIM-homeodomain transcription factor lhx2 (2010)
  • Author(s): Kitajima K
  • Organizer: 5^<th> International Symposium on GATA factors
  • Place of Presentation: Sendai
• [Presentation] Long-term repopulation of adult hematopoiesis by in vitro differentiated blood cells from ESCs/iPSCs by LIM homeobox transcription factor, Lhx2- (2010)
  • Author(s): Kitajima K
  • Organizer: The 8^<th> Stem Cell Research Symposium
  • Place of Presentation: Awaji
• [Presentation] The role of Foxo1 in the development of hematopoietic cells (2010)
  • Author(s): Ken-ichi Minehata
  • Organizer: DECODE Winter Workshop 2010
  • Place of Presentation: 新潟県、湯沢市、Yuzawa Hot Spring
• [Presentation] Induction of in vivo hematopoiesis-repopulating cells from mouse pluripotent stem cells by LIM-homeobox transcription factor, 1hx2 (2010)
  • Author(s): Kenji Kitajima
  • Organizer: DECODE Winter Workshop 2010
  • Place of Presentation: 新潟県、湯沢市、Yuzawa Hot Spring
• [Presentation] The role of Foxo1 in the development of hematopoietic cells (2009)
  • Author(s): Minehata K, Kitajima K, Hara T
  • Organizer: The 32nd Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Yokohama
• [Presentation] Establishment and effects of epigenetic pattern regulated by a tissue specific transcription factor (2009)
  • Author(s): Kitajima K, Zheng J, Nakano T
  • Organizer: The 7th Stem Cell Research Symposium
  • Place of Presentation: Tokyo