Molecular analysis of effects of HUB molecules in transcription factor network on cell differentiation

• Project/Area Number: 21591208
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Kumamoto University
• Principal Investigator: SAKAMOTO Hiroshi 熊本大学, 発生医学研究所, 助教 (00347014)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 細胞分化 / 転写因子 / c-myb / 血液細胞 / 血液幹細胞 / リンパ球 / B細胞 / T細胞 / 骨髄球

Research Abstract

I established a new mouse line reporting the endogenious c-Myb protein level. By using these mice, it was evident that various types of hematopoietic progenitors and stem cells expressed c-Myb, some of which have never been reported before. Also, in hematopoietic stem cell population, c-Myb labeles around 90% of them, which presented futures different form c-Myb positive cells.

Research Products

• [Journal Article] Regulated Expression and Role of c-Myb in the Cardiovascular-Directed Differentiation of Mouse Embryonic Stem Cells (2012)
  • Author(s): Ishida M, El-Mounayri O, Kattman S, Zandstra P, Sakamoto H, Ogawa M, Keller G, Husain M*
  • Journal Title: Circ Res Volume: 110 Pages: 253-64
  • Peer Reviewed
• [Journal Article] Regulated Expression and Role of c-Myb in the Cardiovascular-Directed Differentiation of Mouse Embryonic Stem Cells (2012)
  • Author(s): Ishida M, El-Mounayri O, Kattman S, Zandstra P, Sakamoto H, Ogawa M, Keller G, Husain M
  • Journal Title: Circulation Research Volume: 110 Issue: 2 Pages: 253-64
  • DOI: 10.1161/circresaha.111.259499
  • Peer Reviewed
• [Journal Article] ES cell differentiation as a model to study cell biological regulation of vascular development.' Embryonic Stem Cells : The Hormonal Regulation of Pluripotency and Embryogenesis' ed (2011)
  • Author(s): Tsuji-Tamura K, Sakamoto H, and Ogawa M
  • Journal Title: Craig Atwood Pages: 581-606
• [Journal Article] Hematopoiesis from pluripotent stem cell lines(Review) (2010)
  • Author(s): Sakamoto H, K. Tsuji-Tamura and M. Ogawa
  • Journal Title: Int. J. Hematol Volume: 91 Pages: 384-391
  • Peer Reviewed
• [Journal Article] Hematopoiesis from pluripotent stem cell lines (2010)
  • Author(s): Sakamoto H
  • Journal Title: Int J Hematol 91 Pages: 384-91
  • NAID: 10026400949
  • Peer Reviewed
• [Journal Article] Different roles of Foxo1 and Foxo3 in the control of endothelial cell morphology (2009)
  • Author(s): Matsukawa M
  • Journal Title: Genes Cells 14 Pages: 1167-81
  • Peer Reviewed
• [Journal Article] Foxo1 is essential for in vitro vascular formation from embryonic stem cells (2009)
  • Author(s): Park SH
  • Journal Title: Biochem Biophys Res Commun 390 Pages: 861-6
  • Peer Reviewed
• [Presentation] Bone Marrow Hematopoiesis from ES Cell-Derived Progenitor Cells (2011)
  • Author(s): 坂本比呂志、田村潔美、小川峰太郎
  • Organizer: CREST/さきがけ「iPS細胞」研究領域合同シンポジウム
  • Place of Presentation: 東京日本科学未来館
  • Year and Date: 2011-01-14
• [Presentation] Bone Marrow Hematopoiesis from ES Cell-Derived Progenitor Cells (2011)
  • Author(s): 坂本比呂志
  • Organizer: CREST/さきがけ「iPS細胞」研究領域合同シンポジウム
  • Place of Presentation: 東京(日本科学未来館)
  • Year and Date: 2011-01-14
• [Presentation] The role of Foxo1 transcription factor in vascular development (2011)
  • Author(s): 田村-辻潔美、坂本比呂志、朴勝煥、小川峰太郎
  • Organizer: 第8回心血管幹細胞研究会
  • Place of Presentation: 東京品川プリンスホテル
• [Presentation] Thrombopoietin controls proliferation of embryonic multipotent haematopoietic progenitors via JAK2-STAT5 (2009)
  • Author(s): Sakamoto H
  • Organizer: International Symposium on "Cell Fate Regulation Research : Molecular Basis and Therapeutic Potentials"
  • Place of Presentation: 熊本 熊本ホテルキャッスル
• [Presentation] Establishment of c-myb reporter ES cells (2009)
  • Author(s): Sakamoto H
  • Organizer: International Joint Symposium "Cell Fate Regulation Research : Stem cells and organogenesis"
  • Place of Presentation: 熊本 熊本大学 発生医学研究所
• [Remarks]
  • URL: http://www.imeg.kumamoto-u.ac.jp/divisions/cell_differentiation/