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A whole genomic analysis of acute myeloid leukemia.

Research Project

Project/Area Number 21591217
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionJichi Medical University

Principal Investigator

YAMASHITA Yoshihiro  自治医科大学, 医学部, 准教授 (10326861)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords急性骨髄性白血病 / Blast Bank / ゲノムリシークエンス / 染色体構造異常 / miRNA / 体細胞変異 / アレイCGH
Research Abstract

Leukemias are clonal disorders of hematopoietic stem cells or immature progenitors. However, a simple comparison of the bone marrow(BM) mononuclear cells(MNCs) among heterogeneous acute myeloid leukemia(AML) patients is likely to reveal a large number of changes in the genome that reflect differences either in the percentage of blasts within BM, or in the differentiation ability of the blasts. To minimize such population-shift effects, we have set up a large-scale cell bank(the Blast Bank) for the storage of hematopoietic stem cell(HSC)-like fractions purified from individuals with a wide range of leukemic disorders. To identify oncogenes in leukemias, and to clarify a molecular signature that was directly linked to the prognosis of leukemic patients, we performed a large-scale genomic analysis of the Blast Bank specimens.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (27 results)

All 2012 2011 2010 2009 Other

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (10 results)

  • [Journal Article] High-throughput resequencing of target-captured cDNA in cancer cells2012

    • Author(s)
      Ueno, T., Yamashita, Y., et al.(全9名、第2著者)
    • Journal Title

      Cancer science

      Volume: 103 Issue: 1 Pages: 131-135

    • DOI

      10.1111/j.1349-7006.2011.02105.x

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Oncogenic MAP2K1 mutations in human epithelial tumors2012

    • Author(s)
      Choi, Y. L., Soda, M., et al.(全11名、第10著者)
    • Journal Title

      Carcinogenesis

      Volume: 33 Issue: 5 Pages: 956-961

    • DOI

      10.1093/carcin/bgs099

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ex vivo expansion of human CD8+T cells using autologous CD4+T cell help2012

    • Author(s)
      Butler, M. O., Imataki, O., et al.(全13名、第3著者)
    • Journal Title

      PLoS One

      Volume: 7 Issue: 1 Pages: e30229-e30229

    • DOI

      10.1371/journal.pone.0030229

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Array-based genomic resequencing of human leukemia2010

    • Author(s)
      Yamashita Y, Yuan J, Suetake I, Suzuki H, Ishikawa Y, Choi Y L, Ueno T, Soda M, Hamada T, Haruta H, Takada S, Miyazaki Y, Kiyoi H, Ito E, Naoe T, Tomonaga M, Toyota M, Tajima S, Iwama A, and Mano H
    • Journal Title

      Oncogene

      Volume: 29 Issue: 25 Pages: 3723-3731

    • DOI

      10.1038/onc.2010.117

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] EML4-ALK mutations in lung cancer that confer resistance to ALK inhibitors2010

    • Author(s)
      Choi, Y. L., Soda, M., et al.(全15名、第3著者)
    • Journal Title

      N Engl J Med

      Volume: 363 Issue: 18 Pages: 1734-1739

    • DOI

      10.1056/nejmoa1007478

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of transforming activity of free fatty acid receptor 2 by retroviral expression screening2010

    • Author(s)
      Hatanaka, H., Tsukui, M., et al.(全17名、第7著者)
    • Journal Title

      Cancer science

      Volume: 101 Issue: 1 Pages: 54-59

    • DOI

      10.1111/j.1349-7006.2009.01348.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the transforming activity of Indian hedgehog by retroviral expression screening2010

    • Author(s)
      Hatanaka, H., Takada, S., et al.(全16名、第7著者)
    • Journal Title

      Cancer science

      Volume: 101 Issue: 1 Pages: 60-64

    • DOI

      10.1111/j.1349-7006.2009.01355.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Array-based genomic resequencing of human leukemia.2010

    • Author(s)
      Yamashita, Y., et al.
    • Journal Title

      Oncogene

      Volume: 29 Pages: 3723-3731

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] EML4-ALK mutations in lung cancer that confer resistance to ALK inhibitors.2010

    • Author(s)
      Choi, YL., et al.
    • Journal Title

      N Engl J Med.

      Volume: 363 Pages: 1734-1739

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the transforming activity of Indian hedgehog by retroviral expression screening.2010

    • Author(s)
      Hatanaka, H., et al
    • Journal Title

      Cancer Sci.

      Volume: 101 Pages: 60-64

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of transforming activity of free fatty acid receptor 2 by retroviral expression screening2010

    • Author(s)
      Hatanaka, H., et al.
    • Journal Title

      Cancer Sci. 101

      Pages: 54-59

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Screening for genetic abnormalities involved in ovarian carcinogenesis using retroviral expression libraries2009

    • Author(s)
      Wada, T., Yamashita, Y., et al.(全16名、第2著者)
    • Journal Title

      Int J Oncol

      Volume: 35 Issue: 05 Pages: 973-976

    • DOI

      10.3892/ijo_00000410

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] KIF5B-ALK, a novel fusion oncokinase identified by an immunohistochemistry-based diagnostic system for ALK-positive lung cancer2009

    • Author(s)
      Takeuchi, K., Choi, Y. L., et al.
    • Journal Title

      Clinical cancer research : an official journal of the American Association for Cancer Research

      Volume: 15 Issue: 9 Pages: 3143-3149

    • DOI

      10.1158/1078-0432.ccr-08-3248

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Potential role of miR-29b in modulation of Dnmt3a and Dnmt3b expression in primordial germ cells of female mouse embryos2009

    • Author(s)
      Takada, S., Berezikov, E., et al.(全5名、第4著者)
    • Journal Title

      Rna

      Volume: 15 Issue: 8 Pages: 1507-1514

    • DOI

      10.1261/rna.1418309

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genome-wide histone methylation profile for heart failure2009

    • Author(s)
      Kaneda, R., Takada, S., et al.(全10名、第3著者)
    • Journal Title

      Genes to cells : devoted to molecular & cellular mechanisms

      Volume: 14 Issue: 1 Pages: 69-77

    • DOI

      10.1111/j.1365-2443.2008.01252.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Potential role of miR-29b in modulation of Dnmt3a and Dnmt3b expression in primordial germ cells of female mouse embryos2009

    • Author(s)
      Takada, S., et al.
    • Journal Title

      RNA 15

      Pages: 1507-1514

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Array-based genomic resequencing of human leukemia

    • Author(s)
      Yamashita, Y., et al.
    • Journal Title

      Oncogene (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Array-based genomic resequencing of acute myeloid leukemia2011

    • Author(s)
      Yamashita, Y., et al
    • Organizer
      Cold Spring Harbor Asia conference 2011, Translational Approaches to Cancer meeting
    • Place of Presentation
      中国江蘇省蘇州市
    • Year and Date
      2011-05-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Array-based genomic resequencing of acute myeloid leukemia2011

    • Author(s)
      Yamashita, Y., Ueno, T., Soda, M., Mano, H.
    • Organizer
      Cold Spring Harbor Asia Conference, Translational Approaches to Cancer
    • Place of Presentation
      Victoria, Suzhou, Jiangsu Province, China
    • Related Report
      2011 Final Research Report
  • [Presentation] 大型リシークエンスアレイによる白血病の遺伝子変異スクリーニング2010

    • Author(s)
      山下義博
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪国際会議場(大阪府)
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Array-based genomic resequencing of acute myeloid leukemia.2010

    • Author(s)
      山下義博
    • Organizer
      第16回日本遺伝子治療学会学術総会
    • Place of Presentation
      栃木県総合文化センター(栃木県)
    • Year and Date
      2010-07-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] Array-based genomic resequencing of acute myeloid leukemia2010

    • Author(s)
      Yamashita, Y., et al.
    • Organizer
      Keystone Symposia 2009-2010, Z1 Cancer Meeting
    • Place of Presentation
      カナダブリティッシュコロンビア州ビクトリア
    • Year and Date
      2010-03-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] Array-based genomic resequencing of acute myeloid leukemia2010

    • Author(s)
      山下義博, 間野博行
    • Organizer
      第16回日本遺伝子治療学会学術総会
    • Place of Presentation
      栃木
    • Related Report
      2011 Final Research Report
  • [Presentation] 大型リシークエンスアレイによる白血病の遺伝子変異スクリーニング2010

    • Author(s)
      山下義博, 曽田学, 上野敏秀, 崔永林, 間野博行
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪
    • Related Report
      2011 Final Research Report
  • [Presentation] Array-based genomic resequencing of acute myeloid leukemia2010

    • Author(s)
      Yamashita, Y., Soda, M., Ueno, T., Mano, H.
    • Organizer
      Keystone Symposia, New Paradigms in Cancer Therapeutics, Victoria, British Columbia
    • Place of Presentation
      Canada
    • Related Report
      2011 Final Research Report
  • [Presentation] A large-scale resequencing project of -5600 human genes in the genome of acute myeloid leukemia2009

    • Author(s)
      Yamashita, Y., et al.
    • Organizer
      米国癌学会学術総会(AACR)
    • Place of Presentation
      米国コロラド州デンバー
    • Year and Date
      2009-04-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] A large-scale resequencing project of~5600 human genes in the genome of acute myeloid leukemia2009

    • Author(s)
      Yamashita, Y., Soda, M., Mano, H.
    • Organizer
      AACR 100th Annual Meeting 2009
    • Place of Presentation
      Denver, Colorado, U. S. A.
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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