Generation of bone marrow failure model mice by autoantibodies inducing cytokine production
| Project/Area Number |
21591237
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAO Shinji 金沢大学, 医学系, 教授 (70217660)
KONDO Yukio 金沢大学, 医学系, 助教 (10322116)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ノックアウトマウス / ポリクローナル抗体 / モエシン / 自己抗体 / 骨髄不全 / ハイブリドーマ |
|---|
| Research Abstract |
Moesin KO mice grew normally. However, the body weight of moesin KO mice was lower than that of WT mice. Complete blood counts of moesin KO mice revealed leukocytopenia and macrocytic anemia ; platelet counts were comparable. Moesin KO mouse spleens were heavier than those of WT mice and follicle formation in spleens of moesin KO mice were obscure with increased CD3^+lymphocytes and reduced CD19^+lymphocytes as compared with WT mice. BM of moesin KO mice was normocellular and characterized by increased CD3^+lymphocytes and reduced CD19^+lymphocytes as seen in spleens of moesin KO mice. The number of lineage negative, Sca-1^+, and c-Kit^+(LSK) mouse HSCs in moesin KO BM tended to be lower than that of WT mice. Moesin KO spleen/BM cells capable of producing anti-moesin antibodies were infused into BM of WT B6 male mice. Transplanted mice showed leukocytopenia and increase of TNFαand IFNγin plasma compared to WT B6 male mice transplanted with WT spleen/BM cells, but anemia and thrombocytopenia were not observed.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Aplastic anemia successfully treated with rituximab : the possible role of aplastic anemia-associated autoantibodies as a marker for response2011
Author(s)
Takamatsu, H., Yagasaki, H., Takahashi, Y., Hama, A., Saikawa, Y., Yachie, A., Koizumi, S., Kojima, S. & Nakao, S
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Journal Title
Eur J Haematol
Volume: 86
Pages: 541-545
NAID
Related Report
Peer Reviewed
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