Biological and functional analysis of SFRP1, a novel regulator on early process of lymphopoiesis

• Project/Area Number: 21591241
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Osaka University
• Principal Investigator: YOKOTA Takafumi 大阪大学, 大学院・医学系研究科, 助教 (60403200)
• Co-Investigator(Kenkyū-buntansha): ORITANI Kenji 大阪大学, 大学院・医学系研究科, 准教授 (70324762) ; KANAKURA Yuzuru 大阪大学, 大学院・医学系研究科, 教授 (20177489)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 血液免疫学 / リンパ球前駆細胞 / 造血幹細胞 / Wntシグナル / sFRP / SFRP1 / SFRP5

Research Abstract

We reported that early lymphopoiesis is suspended during pregnant period and high-estrogen condition. By means of intensive analyses on the influence of estrogen on the hematopoietic microenvironment, we have found that soluble Frizzled-related protein 1(SFRP1) acts as a mediator of estrogen action. In the present study, we have constructed transgenic mouse models of SFRP1 or other SFRP-related molecules to evaluate their biological significance in vivo. We have observed severe leukocytopenia, particularly decrease of B-lymphocytes, in SFRP over-expressing mice.

Research Products

• [Journal Article] The endothelial antigen ESAM monitors hematopoietic stem cell status between quiescence and self-renewal (2012)
  • Author(s): Sudo T, Yokota T(13人中2番目), Oritani K(13人中3番目), Kanakura Y(13人中最後), et al
  • Journal Title: J. Immunol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Identification of functional domains and novel binding partners of STIM proteins (2011)
  • Author(s): Saitoh N, Oritani K, Saito K, Yokota T, Ichii M, Sudo T, Fujita N, Nakajima K, Okada M, Kanakura Y
  • Journal Title: J Cell Biochem Volume: 112 Issue: 1 Pages: 147-156
  • DOI: 10.1002/jcb.22910
  • Peer Reviewed
• [Journal Article] Identification of functional domains and novel binding partners of STIM proteins. (2011)
  • Author(s): Saitoh N, Oritani K, Saito K, Yokota T, Ichii M, et al.
  • Journal Title: J.Cell Biochem. Volume: 112 Pages: 147-156
  • Peer Reviewed
• [Journal Article] Kincade PW. Stromal cell-free conditions favorable for human B lymphopoiesis in culture (2010)
  • Author(s): Ichii M, Oritani K, Yokota T, Schultz DC, Holter JL, Kanakura Y
  • Journal Title: J. Immunol. Methods Volume: 359 Issue: 9 Pages: 47-55
  • DOI: 10.1371/journal.pone.0012954
  • Peer Reviewed
• [Journal Article] Kincade PW. The density of CD10 corresponds to commitment and progression in the human B lymphoid lineage (2010)
  • Author(s): Ichii M, Oritani K, Yokota T, Zhang Q, Garrett KP, Kanakura Y
  • Journal Title: PLoS One Volume: 5 Issue: 1-2 Pages: 47-55
  • DOI: 10.1016/j.jim.2010.06.002
  • Peer Reviewed
• [Journal Article] Stromal cell-free conditions favorable for human B lymphopoiesis in culture. (2010)
  • Author(s): Ichii M, Oritani K, Yokota T, et al
  • Journal Title: J.Immunol.Methods. Volume: 359 Pages: 47-55
  • Peer Reviewed
• [Journal Article] The density of CD10 corresponds to commitment and progression in the human B lymphoid lineage. (2010)
  • Author(s): Ichii M, Oritani K, Yokota T, at al
  • Journal Title: PLoS One Volume: 5
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): 横田貴史, 織谷健司, 金倉譲
  • Journal Title: Annual Review 2010血液(中外医学社) Pages: 12-21
• [Journal Article] The endothelial antigen ESAM marks primitive hematopoietic progenitors throughout life in mice (2009)
  • Author(s): Yokota T, Oritani K, Butz S, Kokame K, Kincade PW, Miyata T, Vestweber D, Kanakura Y.
  • Journal Title: Blood Volume: 113 Issue: 13 Pages: 2914-2923
  • DOI: 10.1182/blood-2008-07-167106
  • Peer Reviewed
• [Journal Article] The endothelial antigen ESAM marks primitive hematopoietic progenitors throughout life in mice (2009)
  • Author(s): Yokota T, et al.
  • Journal Title: BLOOD 113 Pages: 2914-2923
  • Peer Reviewed
• [Journal Article] 新たな造血幹細胞マーカーESAM (2009)
  • Author(s): 横田貴史, 織谷健司, 金倉譲
  • Journal Title: 血液・腫瘍科 59 Pages: 209-216
  • NAID: 40019064377
• [Presentation] Satb1 Promotes Hematopoietic Stem Cell Differentiation Toward the Lymphoid Lineages (2011)
  • Author(s): Satoh Y, Yokota T, Kondo M, Kincade PW, Kouro T, Iida R, Kokame K, Miyata T, Sudo T, Tanaka H, Matsumura I, Oritani K, Kohwi-Shigematsu T, Kanakura Y.
  • Organizer: The American Society of Hematology 53rd Annual meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2011-12-12
• [Presentation] Satb1 Promotes Hematopoietic Stem Cell Differentiation Toward the Lymphoid Lineages (2011)
  • Author(s): Yusuke Satoh, Takafumi Yokota, et al
  • Organizer: The American Society of Hematology 53rd Annual meeting
  • Place of Presentation: San Diego Convention Center, San Diego, USA
  • Year and Date: 2011-12-12
• [Presentation] The endothelial antigen ESAM monitors reversible conversion of hematopoietic stem cells between dormancy and self-renewal (2011)
  • Author(s): Sudo T, Yokota T, Sugiyama T, Ishida T, Satoh Y, Oritani K, Nagasawa T, Kanakura Y.
  • Organizer: The American Society of Hematology 53rd Annual meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2011-12-11
• [Presentation] The endothelial antigen ESAM monitors reversible conversion of hematopoietic stem cells between dormancy and self-renewal (2011)
  • Author(s): Takao Sudo, Takafumi Yokota, et al
  • Organizer: The American Society of Hematology 53rd Annual meeting
  • Place of Presentation: San Diego Convention Center, San Diego, USA
  • Year and Date: 2011-12-11
• [Presentation] Satb1 directs hematopoietic stem cell differentiation toward the lymphoid lineages (2011)
  • Author(s): Yokota T, Satoh Y, Kondo M, Kincade PW, Kouro T, Iida R, Sudo T, Tanaka H, Matsumura I, Oritani K, Kohwi-Shigematsu T, Kanakura Y.
  • Organizer: 第40回日本免疫学会総会
  • Place of Presentation: 幕張
  • Year and Date: 2011-11-29
• [Presentation] Satb1 directs hematopoietic stem cell differentiation toward the lymphoid lineages (2011)
  • Author(s): Takafumi Yokota, Yusuke Satoh, et al
  • Organizer: 第40回日本免疫学会総会
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2011-11-29
• [Presentation] SATB1 Induces Early Lymphocyte Differentiation From Primitive Hematopoietic Progenitors (2011)
  • Author(s): Satoh Y, Yokota T, Kondo M, Kokame K, Miyata T, Sudo T, Tanaka H, Matsumura I, Oritani K, Kanakura Y.
  • Organizer: 第73回日本血液学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-15
• [Presentation] SATB1 Induces Early Lymphocyte Differentiation From Primitive Hematopoietic Progenitors (2011)
  • Author(s): Yusuke Satoh, Takafumi Yokota, et al
  • Organizer: 第73回日本血液学会総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-10-15
• [Presentation] A Chromatin Modifier SATB1 Promotes Lymphocyte Production From Primitive Hematopoietic Stem/ Progenitor Cells (2010)
  • Author(s): Satoh Y, Yokota T, Tanaka H, Kokame K, Miyata T, Matsumura I, Oritani K, Kanakura Y.
  • Organizer: The American Society of Hematology 52nd Annual meeting
  • Place of Presentation: Orlando, USA.
  • Year and Date: 2010-12-06
• [Presentation] A Chromatin Modifier SATB1 Promotes Lymphocyte Production From Primitive Hematopoietic Stem/Progenitor Cells. (2010)
  • Author(s): Satoh Y., Yokota T., Tanaka H., et al.
  • Organizer: The American Society of Hematology 52nd Annual meeting
  • Place of Presentation: Orange County Convention Center, Orlando, USA
  • Year and Date: 2010-12-06
• [Presentation] Deletion of core fucosylation on pre-BCR down-regulates its functions (2010)
  • Author(s): Li W, Wang H, Liu Q, Ishihara K, Yokota T, Gu J, Taniguchi N, Kondo A.
  • Organizer: The 14th International Congress of Immunology
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2010-08-25
• [Presentation] Deletion of core fucosylation on pre-BCR down-regulates its functions. (2010)
  • Author(s): Li W., Wang H., Liu Q., Ishihara K., Yokota T., et al.
  • Organizer: The 14th International Congress of Immunology
  • Place of Presentation: Kobe International Exhibition Hall & Portopia Hotel, Kobe, Japan
  • Year and Date: 2010-08-25
• [Presentation] The endothelial antigen ESAM marks hematopoietic stem/ progenitor cells throughout ontogeny in mice (2009)
  • Author(s): Yokota T, Oritani K, Sudo T, Butz S, Kokame K, Kincade PW, Miyata T, Vestweber D, Kanakura Y.
  • Organizer: 第71回日本血液学会総会
  • Place of Presentation: 京都
  • Year and Date: 2009-10-24
• [Presentation] The endothelial antigen ESAM marks hematopoietic stem/progenitor cells throughout ontogeny in mice (2009)
  • Author(s): Yokota T, et al.
  • Organizer: 第71回日本血液学会総会
  • Place of Presentation: 京都 国際会議場
  • Year and Date: 2009-10-23
• [Book] Advances in Hematopoietic Stem Cell Research (2012)
  • Author(s): Takafumi Yokota, Kenji Oritani, Stefan Butz, Stephan Ewers, Dietmar Vestweber, Yuzuru Kanakura
  • Publisher: InTech
  • URL: http://www.intechweb.org
• [Book] Advances in Hematopoietic Stem Cell Research (2012)
  • Author(s): Takafumi Yokota, et al
  • Publisher: InTech-Open Access Company
• [Book] Annual Review血液2010 (2010)
  • Author(s): 横田貴史、織谷健司、金倉譲
  • Publisher: 中外医学社
  • URL: https://www.chugaiigaku.jp