Attempts to answer the crucial qeustion if the anti-tumor cellular immune system can eradicate leukemia stem cells-an analysis using humanized mouse system-
| Project/Area Number |
21591245
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Ehime University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 細胞傷害性Tリンパ球(CTL) / がん抗原特異的T細胞受容体(TCR)遺伝子 / 遺伝子改変Tリンパ球 / 白血病性幹細胞 / 細胞免疫療法 / 同種造血幹細胞移植術 / T細胞受容体(TCR)遺伝子 / 同種造血幹細胞移植 / T細胞受容体遺伝子(TCR)遺伝子 / 遺伝子導入 / 養子免疫療法 / 造血幹細胞移植 |
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| Research Abstract |
Aiming at cure of leukemia, we are developing a novel cellular immunotherapy using genetically modified T lymphocytes to express leukemia antigen specific T-cell receptor(TCR) which can recognize leukemia stem cells(LSCs). In this study, we have demonstrated the clinical feasibility of two therapeutic options targeting Wilms' tumor gene product 1(WT1) and Aurora kinase A(AURKA), and the usefulness of a novel TCR gene expression vector(siTCR vector) which simultaneously encodes silencers for endogenous TCRs. Currently, this on-going study using a xenografted NOG mouse model are accumulating positive lines of evidence that adoptively transferred LSC-specific CTLs can prohibit leukemia progression in vivo.
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Report
(4 results)
Research Products
(136 results)
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[Journal Article] Aurora kinase A-specific T-cell receptor gene transfer redirects T-lymphocytes to display effective anti-leukemia reactivity2012
Author(s)
Nagai K, Ochi T, Fujiwara H, An J, Shirakata T, Mineno J, Kuzushima K, Shiku H, Melenhorst J, Gostic E, Price D. A., Ishii E, Yasukawa M
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Journal Title
Blood.
Volume: 119(2)
Pages: 368-76
Related Report
Peer Reviewed
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[Journal Article] Gemcitabine enhances Wilms' tumor gene WT1 expression and sensitizes human pancreatic cancer cells with WT1-specific T-cell-mediated antitumor immune response2011
Author(s)
Takahara A, Koido S, Ito M, Nagasaki E, Sagawa Y, Iwamoto T, Komita H, Ochi T, Fujiwara H, Yasukawa M, Mineno J, Shiku H, Nishida S, Sugiyama H, Tajiri H, Homma S
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Journal Title
Cancer Immunol Immunother.
Volume: 60(9)
Pages: 1289-97
Related Report
Peer Reviewed
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[Journal Article] Activation of T-cell receptor signaling in peripheral T-cell lymphoma cells plays an important role in the development of lymphoma-associated hemophagocytosis2011
Author(s)
An J, Fujiwara H, Suemori K, Niiya T, Azuma T, Tanimoto K, Ochi T, Akatsuka Y, Mineno J, Ozawa H, Ishikawa F, Kuzushima K, Yasukawa M
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Journal Title
Int J Hematol.
Volume: 93(2)
Pages: 176-185
NAID
Related Report
Peer Reviewed
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[Journal Article] Generation of functional human T-cell subsets with HLA-restricted immune responses in HLA class I expressing NOD/SCID/IL2r gamma(null) humanized mice2010
Author(s)
Shultz LD, Saito Y, Najima Y, Tanaka S, Ochi T, Tomizawa M, Doi T, Sone A, Suzuki N, Fujiwara H, Yasukawa M, Ishikawa F
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Journal Title
Proc Natl Acad Sci USA.
Volume: 107(29)
Pages: 13022-7
Related Report
Peer Reviewed
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