Regulation of differentiation and proliferation of granulocytic precursors by C/EBP transcription factor

• Project/Area Number: 21591246
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Kyoto University
• Principal Investigator: HIRAI Hideyo 京都大学, 医学研究科, 助教 (50315933)
• Research Collaborator: TENEN Daniel g National University of Singapore, Harvard Stem Cell Institute Cancer Science Institute
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 顆粒球 / 転写因子 / C/EBP / 分化 / 増殖 / 感染 / C / EBP

Research Abstract

We developed a novel flow cytometric method that successfully dissected mouse bone marrow cells undergoing granulopoiesis and found that C/EBPβis involved in the efficient amplification of early granulocyte precursors by regulating proliferation during candidemia-induced' emergency' granulopoiesis.

Research Products

• [Journal Article] Rapid automated detection of ABL kinase domain mutations in imatinib-resistant patients (2011)
  • Author(s): Tanaka, R., Kimura, S., Ashihara, E., Yoshimura, M., Takahashi, N., Wakita, H., Itoh, K., Nishiwaki, K., Suzuki, K., Nagao, R., Yao, H., Hayashi, Y., Satake, S., Hirai, H., Sawada, K-I., Ottmann, O. G., Melo, J. V.
  • Journal Title: Cancer Letter Volume: 312(2) Issue: 2 Pages: 228-34
  • DOI: 10.1016/j.canlet.2011.08.009
  • Peer Reviewed
• [Journal Article] Rakicidin A effectively induces apoptosis in hypoxia adapted Bcr-Abl positive leukemic cells (2011)
  • Author(s): Takeuchi M, Ashihara E, Maekawa T, et al
  • Journal Title: Cancer Sci Volume: 102 Issue: 3 Pages: 591-6
  • DOI: 10.1111/j.1349-7006.2010.01813.x
  • NAID: 10029291246
  • Peer Reviewed
• [Journal Article] Galection-9 ameliorates acute Graft-versus-host disease through the induction of T-cell apoptosis (2011)
  • Author(s): Sakai K, Ashihara E, Maekawa T, et al
  • Journal Title: Eur J Immunol Volume: 41 Issue: 1 Pages: 67-75
  • DOI: 10.1002/eji.200939931
  • Peer Reviewed
• [Journal Article] Growth inhibition of imatinib-resistant CML cells with the T315 mutation and hypoxia-adaptation by AV65 。Va novel Wnt/υ-catenin signaling inhibitor (2011)
  • Author(s): Nagao, R., Ashihara, E., Kimura, S., Strovel, J. W., Yao, H., Takeuchi, M., Tanaka, R., Hayashi, Y., Hirai, H., Padia, J., Strand, K.
  • Journal Title: Cancer Letter Volume: 312(1) Issue: 1 Pages: 91-100
  • DOI: 10.1016/j.canlet.2011.08.002
  • Peer Reviewed
• [Journal Article] Use of bicistronic vectors in combination with flow cytometry to screen for effective small interfering RNA target sequences (2010)
  • Author(s): Kamio N, Hirai H, Ashihara E, Tenen DG, Maekawa T, Imanishi J
  • Journal Title: Biochem Biophys Res Commun Volume: 393 Issue: 3 Pages: 498-503
  • DOI: 10.1016/j.bbrc.2010.02.033
  • NAID: 120002426875
  • Peer Reviewed
• [Journal Article] Identification of a myeloid committed progenitor as the cancer initiating cellin acute promyelocytic leukemia (2009)
  • Author(s): Guibal FC, Alberich-Jorda M, Hirai H, Ebralidze A, Levantini E, Di Ruscio A, Zhang P, Santana-Lemos BA, Neuberg D, Wagers AJ, Rego EM, Tenen DG
  • Journal Title: Blood Volume: 114(27) Issue: 27 Pages: 5415-5425
  • DOI: 10.1182/blood-2008-10-182071
  • Peer Reviewed
• [Journal Article] Toll-like receptors(TLRs) are expressed by myeloid leukaemia cell lines, but fail to trigger differentiation in response to the respective TLR ligands (2009)
  • Author(s): Okamoto M, Hirai H, Taniguchi K, Shimura K, Inaba T, Shimazaki C, Taniwaki M, Imanishi J
  • Journal Title: Br J Haematol Volume: 147(4) Issue: 4 Pages: 585-587
  • DOI: 10.1111/j.1365-2141.2009.07858.x
  • Peer Reviewed
• [Journal Article] C/EBPファミリー転写因子による好中球産生の制御機構 (2009)
  • Author(s): 平位秀世
  • Journal Title: 医学のあゆみTOPICS Volume: 23(12) Pages: 1199-1200
• [Journal Article] Identification of a myeloid committed progenitor as the cancer initiating cell in acute promyelocytic leukemia. (2009)
  • Author(s): Guibal FC, Alberich-Jorda M, Hirai H, Ebralidze A, Levantini E, DiRuscio A, Zhang P, Santana-Lemos BA, Neuberg D, Wagers AJ, Rego EM, Tenen DG.
  • Journal Title: Blood 114 Pages: 5415-5425
  • Peer Reviewed
• [Journal Article] Use of bicistronic vectors in combination with flow cytometry to screen foreffective small interfering RNA target sequences (2009)
  • Author(s): Naoka Kamio, Hideyo Hirail, Eishi Ashihara, Daniel G.Tenen, Taira Maekawa, Jiro Imanishi
  • Journal Title: Biochemical and Biophysical Research Communi cations 393 Pages: 498-503
  • NAID: 120002426875
  • Peer Reviewed
• [Journal Article] C/EBPファミリー転写因子による好中球産生の制御機構 (2009)
  • Author(s): 平位秀世
  • Journal Title: 医学のあゆみ 2, 31 Pages: 1199-1200
• [Presentation] BCR/ABL-Mediated Myeloid Expansion Is Promoted by C/EBPβ, a Regulator of Emergency Granulopoiesis (2011)
  • Author(s): Hayashi Y, et al
  • Organizer: 53rd ASH Annual Meeting and Exposition
  • Place of Presentation: San Diego, USA
  • Year and Date: 2011-12-12
• [Presentation] BCR/ABL-Mediated Myeloid Expansion Is Promoted by C/EBPβ, a Regulator of Emergency Granulopoiesis (2011)
  • Author(s): Hayashi Y, Hirai H, Yao H, Yoshioka S, Satake S, Kamio N, Miura Y, Ashihara E, Fujiyama Y, Tenen DG, Maekawa T
  • Organizer: 53rd ASH Annual Meeting and Exposition
  • Place of Presentation: San Diego, US
  • Year and Date: 2011-11-13
• [Presentation] Involvement of C/EBPβin BCR/ABL-dependent leukemogenesis (2011)
  • Author(s): Hayashi Y, Hirai H, Kamio N, Yao H, Satake S, Nagao R, Miura Y, Ashihara E, Maekawa T
  • Organizer: 第73回日本血液学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-15
• [Presentation] Involvement of C/EBPβ in BCR/ABL-dependent leukemogenesis (2011)
  • Author(s): Hayashi Y, et al
  • Organizer: 第73回日本血液学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-15
• [Presentation] Candidemia-Induced emergency granulopoiesis consists of successive dual waves triggered by the shift from C/EBP alpha to C/EBP beta dependency (2010)
  • Author(s): Satake S, Hirai H, Shime N, Nagao R, Tanaka R, Yao H, Hayashi Y, Ashihara E, Inaba T, Fujita N, Imanishi J, Maekawa T
  • Organizer: The American Society of Hematology 52st Annual Meeting and Exposition(ASH)
  • Place of Presentation: Orange County Convention Center, Orlando, FL, USA
  • Year and Date: 2010-12-06
• [Presentation] Candidemia-Induced emergency granulopoiesis consists of successive dual waves triggered by the shift from C/EBPα to C/EBP β dependency (2010)
  • Author(s): Satake S, et al.
  • Organizer: The American Society of Hematology 52st Annual Meeting and Exposition
  • Place of Presentation: オーランド・米国
  • Year and Date: 2010-12-06
• [Presentation] Involvement of CREBs in the regulation of C/EBPβduring "emergency" granulopoiesis (2010)
  • Author(s): Hirai H, Kamio N, Matsusue A, Ogino S, Kimura N, Satake S, Tanaka R, Nagao R, YaoH, Hayashi Y, Takeuchi M, Ashihara E, Huang G, Tenen DG, Imanishi J, Maekawa T
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2010-09-24
• [Presentation] 骨髄系細胞の分化と機能制御 (2010)
  • Author(s): 佐竹早紀子、平位秀世、志馬伸朗、長尾里奈、田中瑠璃子、八尾尚幸、林嘉宏、武内美紀、芦原英司、稲葉亨、藤田直久、今西二郎、前川平
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2010-09-24
• [Presentation] A flow cytometry-based method for screening of effective small interfering RNA target sequences (2010)
  • Author(s): Kamio N, Hirai H, Satake S, Tanaka R, Nagao R, Yao H, Hayashi Y, Takeuchi M, Ashihara E, Maekawa T
  • Organizer: 第16回日本遺伝子治療学会年次学術集会
  • Place of Presentation: 栃木
  • Year and Date: 2010-07-02
• [Presentation] CREB Is Involved in the Regulation of C/EBPβ; During'emergency' Granulopoiesis (2009)
  • Author(s): Hirai H, Kamio N, Matsusue A, Ogino S, Kimura N, Satake S, Ashihara E, Maekawa T, Tenen DG, Imanishi J
  • Organizer: The American Society of Hematology 51st Annual Meeting and Exposition
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2009-12-05
• [Presentation] C/EBP family転写因子による顆粒球造血の制御 (2009)
  • Author(s): 平位秀世
  • Organizer: 第15回血液科学セミナー
  • Place of Presentation: 東京
  • Year and Date: 2009-11-22
• [Presentation] C/EBP family転写因子による顆粒球造血の制御. (2009)
  • Author(s): 平位秀世
  • Organizer: 第15回 血液科学セミナー
  • Place of Presentation: 東京
  • Year and Date: 2009-11-22
• [Presentation] Involvement of CREB in the regulation of "emergency" granulopoiesis through up regulation of C/EBPβ (2009)
  • Author(s): Hirai H
  • Organizer: Kyoto University Global COE "Center for Frontier Medicine" International Symposium/Retreat
  • Place of Presentation: Hyogo, Japan
  • Year and Date: 2009-11-07
• [Presentation] フローサイトメーターを用いた敗血症における顆粒球造血過程の検討 (2009)
  • Author(s): 藤木早紀子、平位秀世、志馬伸明、稲葉亨、藤田直久、今西二郎
  • Organizer: 第83回日本感染症学会総会
  • Place of Presentation: 東京
• [Presentation] CREB Is Involved in the Regulation of C/EBP & beta ; During 'emergency' Granulopoiesis (2009)
  • Author(s): Hirai H, Kamio N, Matsusue A, Ogino S, Kimura N, Satake S, Ashihara E, Maekawa T, Tenen DG, Imanishi J
  • Organizer: The American Society of Hematology 51st A nnual Meeting and Exposition
  • Place of Presentation: New Orleans, USA
• [Remarks] 平位秀世:好中球造血の二つのモードと疾患.京都がん研究会メールマガジン65(9月号)
• [Remarks] 京都大学医学部附属病院・輸血細胞治療部ホームページ
  • URL: http://dtm.kuhp.kyoto-u.ac.jp/research.html
• [Remarks] 京都大学医学部附属病院輸血細胞治療部ホームページ
  • URL: http://dtm.kuhp.kyoto-u.ac.jp/
• [Remarks]
  • URL: http://www.kuhp.kyoto-u.ac.jp/~dtm/
• [Remarks]
  • URL: http://www.kuhp.kyoto-u.ac.jp/~dtm/