Generation of platelets from human subcutaneous adipose tissues in a culture system

• Project/Area Number: 21591250
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Keio University
• Principal Investigator: MATSUBARA Yumiko 慶應義塾大学, 医学部, 特任講師 (70365427)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 輸血学 / 血小板 / 巨核球 / 脂肪組織 / 造血幹細胞 / 遺伝子ターゲティング / 血小板輸血 / 分化誘導 / トランスクリプトーム解析

Research Abstract

A large amount of platelets were generated from subcutaneous adipose tissues and preadipocytes in a highly-reproducible culture system using established culture media to differentiate of hematopoietic stem cells into platelets. Platelet functions were examined in peripheral platelets and cultured platelets. As compared with hematopoietic stem cell-derived platelets and platelets after 6-7hrs of blood drawing, adipose tissue-derived platelets showed higher functions in platelet activation and thrombus formation.

Research Products

• [Journal Article] Culture of megakaryocytes and platelets from subcutaneous adipose tissue and a preadipocyte cell line (2012)
  • Author(s): Matsubara Y, Murata M, Ikeda Y
  • Journal Title: Methods Mol Biol Volume: 788 Pages: 249-58
  • Peer Reviewed
• [Journal Article] Effect of chronic kidney disease on platelet reactivity to dual-antiplatelet therapy in patients treated with drug-eluting stents (2011)
  • Author(s): Arai T, Kawamura A, Matsubara Y, Yokoyama K, Ikeda Y, Fukuda K, Murata M
  • Journal Title: Heart Vessels
  • Peer Reviewed
• [Journal Article] GSK-3beta negatively regulates megakaryocyte differentiation and platelet production from human bone marrow cells in vitro (2011)
  • Author(s): Ono M, Matsubara Y, Shibano Y, Ikeda Y, Murata M
  • Journal Title: Platelets Volume: 22 Pages: 196-203
• [Journal Article] Low Responsiveness to Antiplatelet Drugs (2011)
  • Author(s): Matsubara Y
  • Journal Title: J Jpn Coll Angiol Volume: 51 Pages: 309-314
  • NAID: 10029767464
• [Journal Article] GSK-3β negatively regulates megakaryocyte differentiation and platelet production from primary human bone marrow cells in vitro (2011)
  • Author(s): Ono M, Matsubara Y, Shibano T, Ikeda Y, Murata M
  • Journal Title: Platelets Volume: 22 Issue: 3 Pages: 196-203
  • DOI: 10.3109/09537104.2010.541959
  • Peer Reviewed
• [Journal Article] GSK-3beta negatively regulates megakaryocyte differentiation and platelet production from human bone marrow cells in vitro. (2011)
  • Author(s): Ono Mayumi, Yumiko Matsubara.Yoshiro Shibano, Yasuo Ikeda, Mitsuru Murata
  • Journal Title: Platelets Volume: 22 Pages: 196-203
  • Peer Reviewed
• [Journal Article] Generation of megakaryocytes and platelets from preadipocyte cell line 3T3-L1, but not the parent cell line 3T3, in vitro (2010)
  • Author(s): Matsubara Y, Suzuki H, Ikeda Y, Murata M
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 402 Pages: 796-800
  • Peer Reviewed
• [Journal Article] 巨核球・血小板のin vitro分化誘導法 (2010)
  • Author(s): 松原由美子
  • Journal Title: 日本血栓止血学会誌 Volume: 21(5) Pages: 509-513
  • NAID: 10027675094
• [Journal Article] Generation of megakaryocytes and platelets from preadipocyte cell line 3T3-L1, but not the parent cell line 3T3, in vitro. (2010)
  • Author(s): Yumiko Matsubara, Hidenori Suzuki, Yasuo Ikeda, Mitsuru Murata
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 402 Pages: 796-800
  • Peer Reviewed
• [Journal Article] Culture of Megakaryocytes and Platelets from Subcutateous Adipose Tissue and Preadipocyte Cell Line (2010)
  • Author(s): Matsubara Y, Murata M, Ikeda Y
  • Journal Title: Methods and Protocols (in press)
• [Journal Article] Generation of megakaryocytes and platelets from human subcutaneous adipose tissues (2009)
  • Author(s): Matsubara Y, Saito E, Suzuki H, Watanabe N, Murata M, Ikeda Y
  • Journal Title: Biochem Biophys Res Commun Volume: 378 Pages: 716-720
  • Peer Reviewed
• [Journal Article] 総説血小板産生機構 (2009)
  • Author(s): 松原由美子
  • Journal Title: 最新医学・新しい診断と治療のABC Volume: 63 Pages: 9-16
• [Journal Article] 総論血小板・凝固線溶系検査の解釈 (2009)
  • Author(s): 松原由美子
  • Journal Title: 日本内科学会誌 Volume: 98 Pages: 19-24
• [Journal Article] Generation of megakaryocytes and platelets from human subcutaneous adipose tissues (2009)
  • Author(s): Matsubara Y, Saito E, Suzuki H, Watanabe N, Murata M, Ikeda Y
  • Journal Title: Biochem Biophys Res Commun 378 Pages: 716-720
  • Peer Reviewed
• [Journal Article] 血小板産生機構 (2009)
  • Author(s): 松原由美子
  • Journal Title: 最新医学・新しい診断と治療のABC 63 Pages: 9-16
• [Journal Article] 血小板・凝固線溶系検査の解釈 (2009)
  • Author(s): 松原由美子
  • Journal Title: 日本内科学会誌 98 Pages: 19-24
  • NAID: 10025192885
• [Journal Article] (2009)
  • Author(s): Matsubara Y, Murata M, Ikeda Y
  • Journal Title: Telomeres : Function, Shortening and Lengtheing(Nova Science Publishers, Inc) Pages: 403-413
• [Presentation] Induction of Megakaryocytes from Fibroblasts by p45NF-E2/Maf (2011)
  • Author(s): Wang Y, Ono Y, Ikeda Y, Okamoto S, Murata M, Poncz M, Matsubara Y
  • Organizer: 53^<rd> The American Society of Hematology
  • Place of Presentation: San Diego(USA)
  • Year and Date: 2011-12-13
• [Presentation] Induction of Megakaryocytes from Fibroblasts by p45NF-E2/Maf (2011)
  • Author(s): Yuhuan Wang, Yukako Ono, Yasuo Ikeda, Shinichiro Okamoto, Mitsuru Murata, Mortimer Poncz, Yumiko Matsubara
  • Organizer: 53^<rd> The American Society of Hematology
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2011-12-13
• [Presentation] Induction of Megakaryocytes from Fibroblasts by p45NF-E2/Maf (2011)
  • Author(s): Ono Y, Matsubara Y, Iida K, Suzuki H, Ikeda Y, Okamoto S, Murata M
  • Organizer: 第73回日本血液学会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2011-10-15
• [Presentation] Differentiation of OP9 Bone Marrow Stroma Cells into Megakaryocytes and Platelets via a p45NF-E2-mediated Mechanism (2011)
  • Author(s): Matsubara Y, Ono Y, Suzuki H, Arai F, Suda T, Murata M, Ikeda Y
  • Organizer: XXIII The International Society on Thrombosis and Haemostasis
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2011-07-25
• [Presentation] Generation of Megakaryocytes from Mouse Subcutaneous Adipose Tissues in vitro (2010)
  • Author(s): Sonoda A, Matsubara Y, Suzuki H, Ikeda Y, Matsuo K, Murata M
  • Organizer: 第72回日本血液学会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2010-09-24
• [Presentation] Generation of Megakaryocytes from Mouse Subcutaneous Adipose Tissues in vitro. (2010)
  • Author(s): Akira Sonoda, Yumiko Matsubara, Hidenori Suzuki, Yasuo Ikeda, Koichi Matsuo, Mitsuru Murata
  • Organizer: 第72回日本血液学会
  • Place of Presentation: 横浜
  • Year and Date: 2010-09-24
• [Presentation] GSK-3 negatively regulates thrombopoiesis in an in vitro normal human CD34-positive cell-derived differentiation system (2009)
  • Author(s): Ono M, Matsubara Y, Shibano T, Ikeda Y, Mitsuru Murata
  • Organizer: XXII The International Society on Thrombosis and Haemostasis
  • Place of Presentation: Boston(USA)
  • Year and Date: 2009-07-14
• [Presentation] GSK-3 negatively regulates thrombopoiesis in an in vitro normal human CD34-positive cell-derived differentiation system (2009)
  • Author(s): 小野眞由美, 松原由美子, 芝野俊郎, 池田康夫, 村田満
  • Organizer: XXII The International Society on Thrombosis and Haemostasis
  • Place of Presentation: Boston
  • Year and Date: 2009-07-14
• [Presentation] An inhibitory role of platelet CD109 in platelet function (2009)
  • Author(s): Matsumoto K, Matsubara Y, Hoshino H, Yokoyama K, Watanabe G, Shibano T, Suzuki N, Ikeda Y, Murata M
  • Organizer: XXII The International Society on Thrombosis and Haemostasis
  • Place of Presentation: Boston(USA)
  • Year and Date: 2009-07-13
• [Presentation] Effect of aspirin administration on GPIb alpha shedding : association of glycocalycin level with platelet sensitivity to aspirin (2009)
  • Author(s): Yamaji K, Matsubara Y, Hoshino H, Suzuki N, Ikeda Y, Murata M
  • Organizer: XXII The International Society on Thrombosis and Haemostasis
  • Place of Presentation: Boston(USA)
  • Year and Date: 2009-07-13
• [Presentation] Could D-dimer in the type 2 diabetic patients be a marker of the macrovascular complications (2009)
  • Author(s): Yokoyama K, Tsukada Y, Matsubara Y, Kawai Y, Oikawa Y, Shimada A, Itoh H, Murata M, Ikeda Y
  • Organizer: XXII The International Society on Thrombosis and Haemostasis
  • Place of Presentation: Boston(USA)
  • Year and Date: 2009-07-13
• [Presentation] An inhibitory role of platelet CD 109 in platelet function (2009)
  • Author(s): Matsumoto K, Matsubara Y, Hoshino H, Yokoyama K, Watanabe G, Shibano T, Suzuki N, Ikeda Y, Murata M
  • Organizer: XXII The International Society on Thrombosis and Haemostasis
  • Place of Presentation: Boston
  • Year and Date: 2009-07-13
• [Presentation] Effect of aspirin administration on GPIb alpha shedding : association of glycocalycin level with platelet sensitivity to aspirin (2009)
  • Author(s): 山地賢三郎, 松原由美子, 星野晴彦, 鈴木則宏, 池田康夫, 村田満
  • Organizer: XXII The International Society on Thrombosis and Haemostasis
  • Place of Presentation: Boston
  • Year and Date: 2009-07-13
• [Presentation] Could D-dimer in the type 2 diabetic patients be a marker of the macrovascular complications (2009)
  • Author(s): Yokoyama K, Tsukada Y, Matsubara Y, Kawai Y, Oikawa Y, Shimada A, Itoh H, Murata M, Ikeda Y
  • Organizer: XXII The International Society on Thrombosis and Haemostasis
  • Place of Presentation: Boston
  • Year and Date: 2009-07-13
• [Presentation] 抗血小板剤の臨床薬理 (2009)
  • Author(s): 松原由美子、村田満
  • Organizer: 弟10回日本検査血液学会
  • Place of Presentation: 甲府
  • Year and Date: 2009-07-05
• [Presentation] 血小板産生におけるGSK-3bの役割: in vitro分化誘導法による検討 (2009)
  • Author(s): 小野眞由美、松原由美子、芝野俊郎、池田康夫、村田満
  • Organizer: 第32回日本血栓止血学会
  • Place of Presentation: リーガロイヤルホテル(北九州)
  • Year and Date: 2009-06-06
• [Presentation] 血小板CD109の血小板機能調節における役割 (2009)
  • Author(s): 松本公宏、松原由美子、星野晴彦、横山健次、芝野俊郎、鈴木則宏、池田康夫、村田満
  • Organizer: 第32回日本血栓止血学会
  • Place of Presentation: リーガロイヤルホテル(北九州)
  • Year and Date: 2009-06-06
• [Presentation] 血小板産生におけるGSK-3βの役割:in vitro分化誘導法による検討 (2009)
  • Author(s): 小野眞由美、松原由美子、芝野俊郎、池田康夫、村田満
  • Organizer: 弟32回日本血栓止血学会
  • Place of Presentation: 北九州
  • Year and Date: 2009-06-06
• [Presentation] 血小板CD109の血小板機能調節における役割 (2009)
  • Author(s): 松本公宏、松原由美子、星野晴彦、横山健次、芝野俊郎、鈴木則宏、池田康夫、村田満
  • Organizer: 弟32回日本血栓止血学会
  • Place of Presentation: 北九州
  • Year and Date: 2009-06-06
• [Presentation] アスピリン服用によるGPIbαのsheddingと血小板機能抑制効果 (2009)
  • Author(s): 山地賢三郎、松原由美子、星野晴彦、鈴木則宏、池田康夫、村田満
  • Organizer: 弟32回日本血栓止血学会
  • Place of Presentation: 北九州
  • Year and Date: 2009-06-05
• [Presentation] ヒト皮下脂肪組織からのin vitro分化誘導による巨核球分化・血小板産生 (2009)
  • Author(s): 松原由美子
  • Organizer: 第32回日本血栓止血学会
  • Place of Presentation: リーガロイヤルホテル(北九州)
  • Year and Date: 2009-06-04
• [Presentation] ヒト皮下脂肪組織からのin vitro分化誘導による巨核球分化・血小板産生 (2009)
  • Author(s): 松原由美子
  • Organizer: 弟32回日本血栓止血学会
  • Place of Presentation: 北九州
  • Year and Date: 2009-06-04
• [Book] Platelets and Megakaryocytes, Methods and Protocols (2011)
  • Author(s): Yumiko Matsubara, Mitsuru Murata, Yasuo Ikeda
  • Total Pages: 500
  • Publisher: Springer(印刷中)