| Project/Area Number |
21591251
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SASAKI Yutaka 関西医科大学, 医学部, 講師 (80425066)
UEMURA Yasushi 関西医科大学, 医学部, 講師 (40364781)
MATSUOKA Yoshikazu 関西医科大学, 医学部, 助教 (70533420)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 造血幹細胞 / 臍帯血 / CD34抗原 / 骨髄内移植法 / 重症免疫不全マウス / 再生医療 / NOGマウス / SRC / 可塑性 / NOD / SCIDマウス |
|---|
| Research Abstract |
We have identified human cord blood-derived very primitive CD34-negative hematopoietic stem cell(CD34-HSC) using the intra-bone marrow injection technique for the first time. In addition, we developed a high resolution purification method for CD34-HSC using 18 lineage-specific antibodies and succeeded to identify positive markers of CD34-HSC. Furthermore, we demonstrated that the CD34-HSC seemed to be more immature than the CD34+CD38-HSC. Based on these data, we propose a novel model of human HSC hierarchy.
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