Characterization of human CD34-negative hematopoietic stem cells and their application for regenerative medicine
| Project/Area Number |
21591251
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SASAKI Yutaka 関西医科大学, 医学部, 講師 (80425066)
UEMURA Yasushi 関西医科大学, 医学部, 講師 (40364781)
MATSUOKA Yoshikazu 関西医科大学, 医学部, 助教 (70533420)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 造血幹細胞 / 臍帯血 / CD34抗原 / 骨髄内移植法 / 重症免疫不全マウス / 再生医療 / NOGマウス / SRC / 可塑性 / NOD / SCIDマウス |
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| Research Abstract |
We have identified human cord blood-derived very primitive CD34-negative hematopoietic stem cell(CD34-HSC) using the intra-bone marrow injection technique for the first time. In addition, we developed a high resolution purification method for CD34-HSC using 18 lineage-specific antibodies and succeeded to identify positive markers of CD34-HSC. Furthermore, we demonstrated that the CD34-HSC seemed to be more immature than the CD34+CD38-HSC. Based on these data, we propose a novel model of human HSC hierarchy.
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Report
(4 results)
Research Products
(91 results)
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[Journal Article] CXCL8 enhances the angiogenic activity of umbilical cord blood-derived outgrowth endothelial cells in vitro2011
Author(s)
Kimura T, Kohno H, Matsuoka Y, Murakami M, Nakatsuka R, Hase H, Yasuda K, Uemura Y, Sasaki Y, Fukuhara S, Sonoda Y
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Journal Title
Cell Biol Int
Volume: 35
Pages: 201-208
Related Report
Peer Reviewed
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[Journal Article] Development of a high resolution purification method for precise functional characterization of primitive human cord blood-derived CD34-negative SCID-repopulating cells2011
Author(s)
Ishii M, Matsuoka Y, Sasaki Y, Nakatsuka R, Takahashi M, Nakamoto T, Yasuda K, Matsui K, Asano H, Uemura Y, Fukuhara S, Sonoda Y
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Journal Title
Exp Hematol
Volume: 39
Pages: 203-213
Related Report
Peer Reviewed
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[Journal Article] In vivo dynamics of human cord blood-derived CD34-SCID-repopulating cells using intra-bone marrow injection2010
Author(s)
Kimura T, Matsuoka Y, Murakami M, Kimura T, Takahashi M, Nakamoto T, Yasuda K, Matsui K, Kobayashi K, Imai S, Asano H, Nakatsuka R, Uemura Y, Sasaki Y, Sonoda Y
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Journal Title
Leukemia
Volume: 24
Pages: 162-168
Related Report
Peer Reviewed
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[Presentation] Prospective isolation and functional characterization of human bone marrow-derived hematopoietic stem cell-supportive mesenchymal stromal cells2010
Author(s)
Matusoka Y, Sasaki Y, Takahashi M, Nakatsuka R, Uemura Y, Inoue M, Ogawa H, Takahashi T, Ishikawa J, Hino M, Sonoda Y
Organizer
The 52nd Annual Meeting of American Society of Hematology
Place of Presentation
Orland, U. S. A
Year and Date
2010-12-07
Related Report
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[Presentation] Prospective isolation and functional characterization of human bone marrow-derived MSCs2010
Author(s)
Matusoka Y, Sasaki Y, Takahashi M, Nakatsuka R, Inoue M, Ogawa H, Takahashi T, Ishikawa J, Hino M, Uemura Y, Sonoda Y
Organizer
第72回日本血液学会
Place of Presentation
横浜
Year and Date
2010-09-24
Related Report
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[Presentation] High resolution purification and characterization of human cord blood-derived CD34-negative SCID-repopulating cells with a very immature phenotype2009
Author(s)
Murakami M, Matusoka Y, Nakatsuka R, Takahashi M, Nakamoto T, Yasuda K, Matsui K, Uemura Y, Sasaki Y, Tsuji T, Fukuhara S, Sonoda Y
Organizer
The 51st Annual Meeting of American Society of Hematology
Place of Presentation
New Orleans, U. S. A
Year and Date
2009-12-07
Related Report
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